Transcript * * * Hz

A
B
*
10
Hz
*
5
20 mV
0,25 s
0
C
ctrl
AA
T3
AA+T3
D
-100
1,0
-50
mV
fractional
activation
*
0,5
*
* *
mV
-100
-50
0
*
0
pA/pF
-5
CTRL
AA
T3
AA+T3
-10
*
Suppl . Figure 1
a Representative action potentials recorded from spontaneously-beating cardiomyocytes
obtained from control cells and from cells treated with either AA, T3 or both. b Box plot
showing the distribution of firing rates recorded from cells either in control (CTR; 2.3 ± 0.4 Hz
n=10) or treated (AA, 2.4 ± 0.3 Hz; n=18; T3, 4.7 ± 0.6 Hz; n=8; AA+T3, 5.8 ± 0.9 Hz; n=9).
Black squares indicate the mean values (*p<0, 05 vs control and AA, 1-way Anova and Fisher
test for Mean comparison). c Plots of mean activation curves for If current (Vhalf, inverse slope
factor (s)) measured in control cells (filled circle; CTR -72.6 ± 2.8 mV, s 8.2±0.7 mV n=11) or
in cells treated with AA (empty rhombus; AA -71.3 ±2.1 mV, s 7.7 ± 0.5 mV n=16;), T3 (filled
rhombus; -64.8 ± 1.6 mV, s 6.4 ± 0.7 mV n=16) or AA+T3 (black and white rhombus; AA+T3 64.7 ± 2.1 mV, s 6.6 ± 0.6 mV n=15). Lines are the best fitting by the Boltzmann equation. d
mean current density-voltage relation under the various conditions (*p<0, 05 vs control and
AA, 1-way Anova and Fisher test for Mean comparison).
Suppl Figure 1
Suppl. Table 1: Primers sequence for qRT-PCR.
Gene
HAND1
NKX2.5-promoter
HAND1-promoter
CASZ1-promoter
Forward/reverse (5’ - 3’)
CTGAACTCAAAAAGACGGATGGT
GCGCCCTTTAATCCTCTTCTC
CCCTGCGTTTAGACTCAGCAT
TGCTCCTCGTTAGCCTGAAAA
GTTGATGAGGCTCTGTCTGTTTTC
CCTGTACTGAACCTACCACCCTTT
GAGAGGAAGGACCAGGTGTTAGG
TAGCAGGGCCTCTTAGCTGTCT
Suppl Table 1