Transcript NFSM-Chickpea Mechanical Harvesting Project

Research Progress - Year 2 (2014-15)
Project
Developing chickpea cultivars suited
to mechanical harvesting and tolerant
to herbicides
Support by
Department of Agriculture & Cooperation,
Ministry of Agriculture, Government of India
Under
National Food Security Mission (NFSM)
Presented by
Pooran Gaur
on behalf of Project Team
Project partners
Breeding: Pooran Gaur, S Srinivasan
IIPR, Kanpur
Partners
Breeding: SK Chaturvedi, Murleedhar Aski
Agronomy: Ummed Singh
IARI, New Delhi
Breeding: Shailesh Tripathi, C Bharadwaj
Agronomy: TK Das
PAU, Ludhiana
Breeding: Sarvjeet Singh, Inderjit Singh
Agronomy: Guriqbal Singh
Engineering: Ritu Dogra, Baldev Dogra,
RAK College of
Agriculture, Sehore
(under RVSKVV)
Breeding: Mohammad Yasin
Agronomy: MD Vyas
UAS-Dharwad
Breeding: A Vijaykumar
Agronomy: M Ganajaxi
RARS-Nandyal
(under ANGRAU)
Breeding: Veera Jayalakshmi
Agronomy: P Muniratnam,
Key objectives
Obj 1: Develop chickpea cultivars suited to mechanical
harvesting.
Obj 2: Modify/adjust combine harvesters to customize for
chickpea harvesting
Obj. 3: Develop chickpea cultivars tolerant to herbicides
Harvesting
Objective 1: Develop chickpea cultivars
suited to mechanical harvesting
Activity 1.1: Evaluate machine harvestable chickpea breeding
lines at farmers’ fields for assessing yield performance and
suitability to combine harvesting.
Activity 1.2: Evaluate machine harvestable chickpea breeding
lines for grain yield in multilocation trials.
Activity 1.3: Evaluate machine harvestable chickpea breeding
lines for grain yield in station trials
Activity 1.4: Targeted breeding for development of cultivars
suited to mechanical harvesting.
Activity 1.5: Develop suitable agronomy for machine
harvestable chickpea varieties.
Activity 1.1: Evaluate machine harvestable chickpea
breeding lines at farmers’ fields
(1) Demonstrations on machine harvestable desi chickpea breeding
line NBeG 47 on farmers’ fields in AP
Year
2013-14
2014-15
No. of demos
JG 11
(Yield kg/ha)
NBeG 47
(Yield kg/ha)
21
12
1616
1575
1731
1608
• NBeG 47 gave yield similar to or higher
than JG 11 and was amenable to machine
harvesting.
• Proposal for release of NBeG 47 submitted
to State Variety Release Committee.
JG 11
NBeG 47
JAKI 9218
(2) Demonstrations on machine harvestable chickpea breeding line
GBM 2 on farmers’ fields in Karnataka during 2014-15
No. of Check
demos variety
Yield of check
(kg/ha)
Yield of GBM
2 (kg/ha)
8
JG 11
1183
1168
2
JAKI 9218
1569
1594
2
Annigeri-1
1235
1421
1
BGD 103
1260
1408
• GBM 2 gave 12-15% higher yield than
Annigeri-1 and BGD 103 and matched yield
levels of popular cultivars JG 11 and JAKI
9218.
• Proposal for release of GBM 2 recommended
by the University level committee and
submitted to State Variety Release
Committee.
Activity 1.2: Evaluate machine harvestable chickpea
breeding lines for grain yield in multilocation trials
Initial Varietal Trial (IVT) – Mechanical Harvesting introduced in
AICRP trials from 2014-15
• 12 Breeding lines contributed by NFSM project partners
Center
IIPR-Kanpur
IARI-New Delhi
PAU-Ludhiana
RARS-Nandyal
UAS-Dharwad
RAKCA-Sehore
Entries contributed
IPC 2008-83, IPC 2011-28, IPC 03205
BG 3061, BG 3062
GL 12003, GL 96836
NBeG 780, NBeG 03108
DBGV 03112
RVSSG 08102, RVSSG 08103
Mechanical Harvesting Trial-1 (MHT-1)
Entries selected from MHT-1 and MHT-2 of 2013-14 were evaluated
under MHT-1 during 2014-15
MHT-1A (Northern India)
No. of entries: 28 + one common check HC 5 + one local check
Locations (3): Kanpur, New Delhi and Ludhiana
MHT-1B (Central and southern India)
No. of entries: 22 + 4 checks (JG 11 and JAKI 9218 for yield; GBM 2 and
NBeG 47 for plant height)
Locations (4): Sehore, Patancheru, Nandyal, Bijapur
For both the trials:
Experimental design: RCBD with 3 reps
Plot size: 4 row of 4m
MHT-1B: Promising lines identified based on 1st pod height and
grain yield……….(1/2)
(1) Patancheru
(2) Nandyal
1st pod height
Yield
(kgha-1)
Entry
ICCV 08101
32.8
2346.3
ICCV 13606
34.6
2309.1
ICCV 08102
30.7
2246.2
ICCV 05103
33.1
2241.8
ICCV 11516
32.2
2172.6
ICCV 13647
32.9
2169.2
ICCV 13601
41.6
2094.8
NBeG 47
34.3
GBM 2
Best check
Entry
1st pod height
Yield
(kgha-1)
ICCV 05103
37.8
3526.0
ICCV 03108
33.5
3513.5
2275.4
NBeG 47
36.7
2972.1
29.4
2100.2
GBM 2
31.1
2684.4
23.1
2393
(JG 11)
23.3
3588
(JG 11)
LSD 5%
323
299
CV %
8.2
6.1
MHT-1B: Promising lines identified based on 1st pod height and
grain yield……….(2/2)
(4) Sehore
(3) Bijapur
1st Pod height
Yield
(kgha-1)
Entry
1st Pod height
Yield
(kgha-1)
ICCV 08102
27.9
2248.7
ICCV 07112
30.1
2958.2
ICCV 03205
26.5
2058.7
ICCV 08102
36.7
2449.9
ICCV 03104
26.9
1959.1
ICCV 07104
33.9
2388.8
ICCV 05103
29.1
1717.1
ICCV 13601
48.3
2305.5
ICCV 08101
27.8
1557.0
ICCV 13647
39.9
2091.6
ICCV 05103
30.2
2033.0
ICCV 13606
30.1
1822.1
ICCV 11516
36.7
1758.3
ICCV 13605
43.7
1427.7
Entry
NBeG 47
30.6
1572.0
NBeG 47
33.9
1330.2
GBM 2
26.5
1558.1
GBM 2
29.7
950.0
Best check
16.4
1778 (JG 11)
21.7
1583 (JG 11)
LSD 5%
215
189
CV %
8.0
5.9
MHT-1A: Promising lines selected based on 1st pod height and grain yield
(1) Kanpur
(2) New Delhi
st
Entry
1 pod
height
(cm)
Yield
Entry
(kgha-1)
(3) Ludhiana
1st
pod
height
(cm)
Yield
Entry
(kgha-1)
1st pod
height
(cm)
Yield
(kgha-1)
IPC 2012-164
39.3
1520
ICCV 11514
28.8
1567.4 ICCV 96839
53.0
2249.9
IPC 2012-253
33.7
1441
ICCV 13621
29.3
1499.5 ICCV 13645
51.3
2041.6
ICCV 13617
32.7
1311
ICCV 13628
31.4
1492.4 ICCV 11516
48.3
1819.4
ICCV 08101
31.0
1191
Pusa 547
26.0
1482.4 ICCV 13621
52.3
1638.8
ICCV 13645
25.8
1465.2
ICCV 96839
27.0
1449.3
ICCV 13619
27.3
1405.1
ICCV 13623
31.1
1385.0
ICCV 08101
29.6
1379.8
ICCV 11601
29.4
1357.5
25.7
1556
(HC-5)
42.0
1833
(PBG-5)
Best check
36.3
1038
(IPC 92-3)
LSD 5%
-
196
184
CV %
-
8.8
8.8
Mechanical Harvesting Trial-2 (MHT-2)
Entries selected from station trials (MHT-3) of 2013-14 were
evaluated under MHT-2 during 2014-15
No. of entries: 40 + 4 checks (JG 11, JAKI 9218, HC 5 and
local check)
Locations: All 7 locations
Experimental design: RCBD with 3 reps
Plot size: 1 row of 4m
MHT-2B: Promising lines identified based on 1st pod height and
grain yield……….(1/2)
Patancheru
Nandyal
1st pod height
(cm)
Yield
(kgha-1)
1st pod
height (cm)
Yield
(kgha-1)
Entry
ICCV 13108
32.2
3117.9
ICCV 03105
43.7
4409.6
ICCV 13107
31.4
2955.7
N BeG 780
47.3
4262.0
ICCV 97127
31.4
2666.8
ICCV 13635
42.8
4008.3
N BeG 780
38.9
2457.5
ICCV 13640
50.7
2442.8
ICEL - 5
41.3
2411.1
ICCV 13114
33.2
2372.0
ICEL - 2
34.6
2237.3
GL 13016
34.4
2072.7
ICCV 13631
36.4
1909.0
NBeG 47
39.9
4425.3
26.1
2089
(JG 11)
34.5 cm
2928
(JG 11)
Entry
Best check
LSD 5%
371
336
CV %
9.7
6.5
MHT-2B: Promising lines identified based on 1st pod height and
grain yield……….(2/2)
Sehore
Bijapur
1st Pod height
(cm)
Yield
(kg/ha)
1st Pod height
(cm)
Entry
Yield
(kg/ha)
ICEL - 5
28.1
1419.5
ICCV 13631
43.7
2477.2
GL 13044
24.7
1396.4
ICCV 13611
40.9
2272.1
ICCV 13114
26.4
1380.8
GL 13016
53.2
2247.7
ICEL - 4
22.3
1355.8
ICCV 13634
40.2
2243.6
ICCV 11515
24.4
1329.4
ICCV 13616
31.0
2213.8
ICEL - 1
21.8
1314.3
GL 12021
43.8
2124.9
ICCV 13625
28.4
1294.4
ICCV 13108
33.6
2108.2
ICCV 01301
22.2
1281.1
ICCV 13608
45.2
1847.1
ICCV 13630
24.3
1187.7
ICCV 13633
44.9
1833.3
ICCV 03105
24.4
1175.2
ICCV 13604
42.9
1722.2
HC-5
29.7
1233.6
HC 5
33.3
1683.3
17.8
987.7
(JG 11 )
25.0
1127.7
(JG 11)
Entry
Best check
LSD 5%
171
201
CV %
9.3
7.2
MHT-2 in Northern India
Kanpur
• Trial severely affected by winter rains.
• None of the entries out yielded the best check DCP 92-3 (2060 kg/ha), which
is released for high input conditions. The check HC 5 yielded 1425 kg/ha.
• Promising entries included IPC 2011-28 (2060 kg/ha), IPC 2012-49 (2083
kg/ha), IPC 2011-85 (2163 kg/ha), IPC 2012-31 (2167 kg/ha), and IPC 2010142 (2222 kg/ha)
New Delhi
• Trial severely affected by winter rains and quality data could not be collected
due to lodging.
• None of the entries could surpass local check HC 5
Ludhiana
• Trial severely affected by winter rains and ascochyta blight . Eleven
genotypes severely affected and did not produce seed. The most promising
genotypes were GL 12021 (2.3 t/ha), GL 13016 (2.2 t/ha) , ICCV 13604 (2.1
t/ha).
Activity 1.3: Evaluate machine harvestable chickpea
breeding lines for grain yield in station trials
Breeding lines evaluated in MHT-3 (station trial) at different locations
Location
ICRISATPatancheru
IIPR-Kanpur
No. of
entries
144
Remarks
40 promising lines identified.
27 + 3 checks IPC 2011-28 (2060 kg/ha), IPC 2012-49 (2083 kg/ha), IPC
(HC 5, IPC 92- 2011-85 (2163 kg/ha), IPC 2012-31 (2167 kg/ha) and IPC
3, JG 16)
2010-142 (2222 kg/ha) outyielded the check HC 5 (1425
kg/ha).
IARI-New Delhi
PAU-Ludhiana
30
24
RAKCA-Sehore
76 (Set-1)
34
(Set-II)
UAS-Dharwad
12
3 lines gave 10% more yield than the best check (HC 5)
The genotypes GL14009, GL14010, GL14011, GL14012, GL14049,
GL14050, GL14053, GLI4057 were found promising.
35 promising entries identified
Six promising entries (ICCV 920412, ICCV 96836 x PBG 5, ICC 37 x K
1189, JG 11 x ICCV 05529, HPG 17 x ICCV 96029, Himchana 1 x ICCV
96029) were identified.
Two genotypes (UASD ST2015-05 and ST2015-07) were found
promising
Activity 1.4: Targeted breeding for development of
cultivars suited to mechanical harvesting
Generation
New crosses
F1
F2
F3
F4
F5
ICRISAT,
Patancheru
13
26
28
14
-
IIPR,
IARI, New
PAU,
Kanpur
Delhi
Ludhiana
4
4
6
2
-
4
4
2
3
3
5
7
14
1
3
3
3
RAKCA,
Sehore
RARS,
Nandyal
UAS,
Dharwad
Total
9
13
8
6
2
-
2
3
4
-
3
4
79
30
-
42
68
100
72
22
8
Activity 1.5: Develop suitable agronomy for
machine harvestable chickpea varieties
Experiment: Agronomic evaluation of machine harvestable
genotypes under variable crop geometry (row and plant
spacing)
Treatments (8)
Row spacing: 30 cm, 22.5 cm
Plant spacing: 10 cm, 7.5 cm
Genotypes: Two (GBM 2 and NBeG 47 in central and southern India; HC 5
and one other variety in Northern India)
Experimental design: Split pot with 4 replications
Locations (6): Kanpur, New Delhi, Ludhiana, Sehore, Nandyal,
Dharwad
Results
from row
and plant
spacing
trials –
Central and
southern
India
Results from row and plant spacing trials – Northern India
Note: Trial affected by Ascochyta blight at PAU-Ludhiana
Objective 2: Modify/adjust combine harvesters to
customize for chickpea harvesting
Activity 2.1: Evaluate the available commercial harvesting
machines and make required modifications/ adjustments to
customize for chickpea harvesting.
Harvesting by two Combine harvester
(A) Harvesting with CLAAS combine harvester
(B) Harvesting with STANDARD S-390 combine with two cutter bar width 3 m
and 4.2 m.
Parameters
Details
Crop Varieties
HC-5, PBG-5, NBeG-47
Harvested Area
0.9
Cylinder speed, m/s
PS1 (13.54) & PS2 (15.12)
Forward speed, km/h F1(1.2)
Brief specifications of the STANDARD S-390 combine harvester
S.No.
Particular
Specification
1
Combine harvester
STANDARD S-390
2
Power source, hp rpm
50 to 55 (Tractor) 2000-2500
3
Cutter bar width, cm
3.0 & 4.2
4
Threshing system
Tangential Flow Type
5
Cylinder width,cm
55
6
Rotor Dia, cm
45
7
Cleaning system
Force air cleaning fan with shutter control
8
Fan speed, rpm
1200-1500
9
Grain tank capacity, lit
1500
10
Unloading system
Universal joint type
11
Overall Dimensions
Weight, kg
4620 kg
Length, mm
6990
Width, mm
2965
Length, mm
3660
Track width, mm
2190
Wheel base, mm
3166
Ground clearance, mm
355
Settings of STANDARD S-390 combine harvester for
chickpea harvesting
S. No.
Particulars
1
Reel position w. r. t. Cutter Height, mm
Forward, mm
bar
200
170
RPM,
10
2
Cutter bar height from ground, cm
0, 5 & 10
3
Cylinder speed, m/s (rpm)
13.54 &15.12
4
Concave clearance, mm
38.1 & 69.85
5
Type of threshing Drum,
Spike tooth
6
Machine forward speed, km/h
7
Opening space of sieve, mm
1.2
Upper
20-50
Lower
5-10
Field Operation of Combine Harvester on Chickpea
(a)
(b)
(c)
Harvesting of chickpea (a) Claas Tangential Axial Flow (b) STANDARD S 390 (3 m
cutter bar) and (c) STANDARD S 390 (4.2 m cutter bar)
Salient findings
Parameters
HC 5
PBG 5
NBeG 47
Shattering
loss (%)
1.5 to 4.1
2.4 to 3.9
3.3 to 3.8
Threshing
efficiency (%)
98.3 to 99.2
98.2 to 99.1
98.2 to 99.5
Grain damage 2.7 to 4.9
(%)
2.4 to 6.2
3.9 to 7.2
Sieve loss (%) 0.8 to 1.1
0.8 to 1.0
0.7 to 0.8
Separation
loss
0.4 to 0.7
0.5 to 0.6
0.2 to 0.3
Cleaning
efficiency
88.7 to 89.4
86.5 to 90.5
88.3 to 90.9
5.3% in manual
harvesting
Assessment of the efficiency of combine harvesting of
NBeG 47 and JG 11 at Nandyal
NBeG 47
JG 11
Performance of combine harvesters at Nandyal
Variety
Parameter
JG-11
Threshing Efficiency, %
89.53
Broken,%
2.77
Shoe Loss,%
1.02
Rake Loss, %
1.41
Cleaning Efficiency, %
92.89
Threshing Efficiency, %
91.11
Broken,%
3.15
Shoe Loss,%
1.59
Rake Loss, %
1.02
Cleaning Efficiency, %
90.14
NBeG-47
Observation
Objective 3: Develop chickpea cultivars
tolerant to herbicides
Activity 3.1: Multilocation evaluation of putative herbicide
tolerant lines selected in previous year.
Activity 3.2: Screen a new set of genotypes (germplasm/
cultivars/breeding lines) for herbicide tolerance in station
trials
Activity 3.3: Develop and screen mutagenic populations
(M2/M3/TILLING) for herbicide tolerance
Activity 3.4: Targeted breeding for developing herbicide
tolerant breeding lines
Activity 3.5: Agronomic trials on weed management by
herbicides
Activity 3.1: Multilocation evaluation of putative herbicide
tolerant lines selected in previous year.
Herbicide Tolerance Screening - 1 (HTS-1)
Entries: 21 genotypes selected from HTS-1 and HTS-2 trials
of last year were evaluated in HTS-1 during 2014-15.
Treatments: Unsprayed (control) and sprayed with
Imazethapyr (Pursuit: BASF)
Experimental Design: RCBD with 4 replications.
Plot size: 4 rows of 4m
Locations: All seven locations
HTS-1: Top genotypes selected based on yield under
sprayed condition……..(1/2)
Patancheru
Entry
Nandyal
Yield
(kg ha-1)
Entry
Bijapur
Yield
(kg ha-1)
Yield
(kg ha-1)
Entry
ICCV 10
1644
ICCIL 01031
4002
ICCIL 01026
1817
ICCV 08109
1561
ICCIL 01034
3826
ICCV 09106
1777
ICCV 09106
1551
ICCV 08102
3351
ICCIL 04021
1749
ICCIL 04021
1548
ICCV 10114
3296
ICCV 08109
1742
ICCIL 04004
1506
ICCV 08109
3251
ICCIL 01034
1736
ICCV 97105
1505
ICCIL 04016
3122
ICCV 10
1709
ICCIL 01034
1339
ICCV 10
3010
ICCV 04516
1625
ICCIL 04007
1303
ICCIL 01026
2972
ICCIL 04004
1607
ICCIL 01026
1277
ICCIL 04004
2863
ICCV 97105
1600
ICC 9567
1056
ICCV 09106
2832
ICCIL 01031
1594
Common at 6 locations
Common at 5 locations
Common at 4 locations
HTS-1: Top genotypes selected based on yield under
sprayed condition……..(2/2)
Sehore
Entry
New Delhi
Yield (kg ha-1) Entry
Ludhiana
Visual score* Entry
Visual score*
ICCV 08102
1572
ICCIL 04021
1.7
ICCIL 01031
2.0
ICCIL 04021
1361
ICCIL 04004
1.9
ICCV 04307
2.1
ICCIL 04016
1254
ICCV 10
2.1
ICCV 08102
2.2
ICCV 09106
1247
ICCIL 04016
2.1
ICCIL 01026
2.2
ICCIL 01034
1197
ICCV 96836
2.8
ICCIL 01034
2.3
ICCIL 01026
1175
ICCIL 04007
2.8
ICCIL 04021
2.3
ICCV 97105
1148
ICCIL 01026
2.8
ICCV 08109
2.5
ICCIL 04007
1129
ICCV 08109
2.9
ICCV 09106
2.7
ICCV 10
1126
ICCV 09106
3.2
ICCV 05530
2.8
ICCIL 01031
1059
ICC 9567
3.2
ICCV 96836
2.8
*Herbicide tolerance score on 1 to 5 score
(1= highly tolerant, 5 highly sensitive)
Common at 6 locations
Common at 5 locations
Common at 4 locations
Herbicide Tolerance Screening - 2 (HTS - 2)
Entries selected from station trials (HTS-3) of 2013-14 were
evaluated under HTS-2 during 2014-15
No. of entries: 100
Herbicide: Imazethapyr (Pursuit: BASF)
Locations: All seven project locations
Experimental design: Alpha Lattice with 4 reps
Plot size: 1 row of 4m
HTS-2: Top genotypes selected based on yield under
sprayed condition……..(1/2)
Patancheru
Entry
Nandyal
Yield
(kg ha-1)
Entry
Bijapur
Yield
(kg ha-1)
Entry
Yield
(kg ha-1)
ICCV 11101
1836
ICCV 11116
2556
ST-3-D-2
2204
ICCV 97007
1772
ICCV 11101
2472
ICCV 93122
1969
ICCV 95013
1764
ICCV 93054
2341
ST-3-D-16
1956
ST-3-D-5
1759
RVG 201
2153
ICCV 97025
1928
N BeG 510
1718
GNG 1958
2074
Pusa 5028
1903
ICCV 95008
1705
NBeG 453
2071
ICCV 91020
1896
ICCV 97056
1676
ST-3-D-1
2032
ICCV 93055
1880
ICCV 11109
1660
ICCV 97025
2011
ICCV 97110
1871
ST-3-D-2
1652
JSC 38
1976
ST-3-D-10
1857
ICCV 11303
1640
ST-3-D-2
1958
ICCV 95008
1827
LSD
238
188
203
CV%
13
7.1
269
Common at least at 2 locations
HTS-2: Top genotypes selected based on yield under
sprayed condition……..(2/2)
Sehore
Entry
Ludhiana
Yield
Entry
(kgha-1)
Visual
score*
ICCV 97049
1570
N BeG 471
2.1
RVG 201
1493
N BeG 119
2.3
ICCV 96005
1397
N BeG 510
2.3
JSC 38
1370
PBG 5
2.3
ICCV 95012
1368
GL 12003
2.3
BGD 128
1354
GL 29098
2.4
RVG 202
1344
BG 2085
2.4
N BeG 458
1302
ICCV 97315
2.4
ICCV 95005
1268
GL 10061
2.5
ICCV 96003
1222
GL 22044
2.5
LSD 5%
140
CV%
8.7
Kanpur
• Trial severely affected by winter
rains.
• GL1006, GL29098, ICCV 93055,
ICCV 97019, ICCV 97017, GL
22044, GLW 12042, GLW 12041,
NBeG 453 appeared promising.
New Delhi
• Trial severely affected by winter
rains and quality data could not be
collected due to lodging
Common at least at 2 locations
*Herbicide tolerance score on 1 to 5 score
(1= highly tolerant, 5 highly sensitive)
Activity 3.2: Screen a new set of genotypes germplasm/cultivars/
breeding lines) for herbicide tolerance in station trials
HTS-3: Each centre screened available cultivars/breeding
lines/germplasm for tolerance to herbicide imazethapyr (Pursuit)
HTS-3: Northern India
Location
IIPR-Kanpur
IARI-New Delhi
PAU-Ludhiana
No. of Crop condition and findings
entries
28
Crop affected by rains; 3 promising lines (IPC
2010-81, IPC 2008-59, IPC 2014-86) identified
34
Crop affected by rain; 6 promising lines (ICC
1205, JAKI 9218, ICC 14402, ICC 12928, ICC 1710
and ICC 637) identified.
Also screened for herbicides Metrabuzin
498
(Sencor) and Carfentrazone (Affinity);
Crop affected by ascochyta blight; 26 promising
lines selected.
HTS-3: Central and Southern India
Location
No. of
entries
152
ICRISATPatancheru
RAKCA-Sehore 200 (Set-I)
50 (Set-II)
RARS-Nandyal 27
ARS-Bijapur
219
Crop conditions and findings
48 promising lines identified.
Over 50 genotypes found promising.
18 promising lines identified.
Top yielding entries viz., N BeG 179 (2616
kg/ha), N BeG 439 (2599 kg/ha), N BeG 511
(2499 kg/ha), and NBeG 402 (2482 kg/ha)
28 promising entries identified
Activity 3.3: Develop and screen mutagenic
populations (M2/M3/TILLING) for herbicide tolerance
(A) New mutagenic populations developed
Location
M1/M2 grown during 2014-15
ICRISAT-Patancheru
M2 of ICCV 10
M2 of DCP 92-3, Shubhra
M1 of Pusa 372
M2 of GPF2
IIPR-Kanpur
IARI-New Delhi
PAU-Ludhiana
RAKCA-Sehore
RARS-Nandyal
UAS-Dharwad
M1 of RVG 202, BGD 112, RVSKG 102 and ICC
4812; M2 of JG 6 and RVSJKG 102
M1 of JAKI 9218
M1 of BGD 103 and BG 1105
(B) Herbicide tolerance screening of mutagenic populations
Location
ICRISATPatancheru
IIPR-Kanpur
IARI-New
Delhi
PAULudhiana
RAKCASehore
RARSNandyal
UASDharwad
Populations screened
M4 of KAK 2, JG 11 and
ICCV 95333
M2 of Shubhra and DCP
92-3
M4 of KAK 2, JG 11 and
ICCV 95333; TILLING
population of ICC 4958
M4 of KAK 2, JG 11 and
ICCV 95333
M2 of JG 6 and RVSJKG
102; M4 of JG 11, KAK 2)
M2 of JAKI 9218; M4 of
KAK 2, JG 11 and ICCV
95333
M4 of JG 11, KAK 2, ICCV
95333
Selection of tolerant plants
Putative tolerant plants selected.
Crop damaged by rains. Plants that
produced pods were bulk harvested.
Putative tolerant plants selected.
Crop damaged by ascochyta blight
Putative tolerant plants selected.
Plants selected: JAKI 9218 44; KAK 2 78,
JG 11 63, ICCV 95333 46.
Plants selected: KAK 2 - 222, ICCV 95333
- 234 & JG 11 - 258
Spray of herbicide
(Pursuit) on M4
population at ICRISAT
M4 population two
weeks after herbicide
spray
Activity 3.4: Targeted breeding for developing herbicide
tolerant breeding lines
Generation
ICRISAT
IIPR
IARI
PAU
RAKCA
RARSNandyal
UASDharwad
Total
New
crosses
6
3
4
7
-
-
2
22
F1
-
3
4
-
-
2
6
15
F2
58
2
-
7
-
-
-
67
F3
-
3
-
-
-
-
-
3
F4
-
7
-
-
-
-
-
7
F5
-
-
-
-
-
-
-
-
Development of mapping populations for herbicide tolerance: Two RIL
populations are being developed for mapping QTL/genes for tolerance to
herbicide imazethapyr (ICC 1710 x ICC 14077) and metribuzin (ICC 1205 x ICC
14077). The RILs were advanced to F4.
Activity 3.5: Agronomic trials on weed management
by herbicides
Genotypes:
ICCV 09311/NBeG 47 (Southern India)
ICCV 10 (Central India)
ICCV 08312 (Northern India)
Treatments:
Imazethapyr @ 50 g/ha and 75 g/ha
Carfentrazone-ethyl @ 20 g/ha and 30 g/ha
Hand weeding
Weedy check
Locations:
RARS-Nandyal (Southern India)
RAKCA-Sehore (Central India)
PAU-Ludhiana, IIPR-Kanpur (Northern India)
Design:
RBD with 4-replications
Results of agronomic trials on weed management
Treatment
PAU
(weed
control %)
IIPR
Yield (kg/ha)
RAKCA
Yield (kg/ha)
RARSNandyal
Yield
(kg/ha)
Imazethapyr
@ 50 g/ha
60.0
1203
2386
2054
Imazethapyr
@ 75 g/ha
66.2
1123
2046
2158
Carfentrazone-ethyl
@ 20 g/ha
63.7
1267
2365
2541
Carfentrazone-ethyl
@ 30 g/ha
67.5
1342
2602
2416
Hand weeding
100.0
1816
2760
2707
Weedy check
-
919
1697
1995
Project management, monitoring and evaluation
Review and Planning Meeting
29 Aug 2014, RARI, Durgapura, Jaipur
Monitoring of field trials
Location
Date
Monitored by
ICRISAT, Patancheru 06/01/2015 Dr. N.P. Singh, Dr. S.K. Chaturvedi, Dr. Sarvjeet Singh, Dr. V.
Jayalakshmi, Dr. Vijay Kumar
ICRISAT, Patancheru 04/04/2015 Dr. G.P. Dixit (PC, Chickpea) and Dr. S.K. Chaturvedi
IARI, New Delhi
03/03/2015 Drs Sarvjeet Singh, Guriqbal Singh and others
IARI, New Delhi
10/04/2015 Dr. Rajeev Varshney and Dr. P.M. Gaur
IIPR, Kanpur
27/02/2015 Dr. U.P. Singh, Member Research Advisory Committee of IIPR
IIPR, Kanpur
02/03/2015 Dr. Sarvjeet Singh and Dr. Guriqbal Singh
IIPR, Kanpur
08/03/2015 Dr. M.C. Diwakar (Director Sugarcane Development)
IIPR, Kanpur
11/04/2015 Dr. Rajeev Varshney, Dr. P.M. Gaur and Dr. M. Yasin
PAU, Ludhiana
06/02/2015 Dr. Sailesh Tripathi
PAU, Ludhiana
12/03/2015 Dr SK Chaturvedi, Dr Mohammad Yasin
PAU, Ludhiana
24/03/2015 Dr SK Yadav, DAC, Govt. of India
RAKCA, Sehore
06/12/2014 Dr. P.M. Gaur
RAKCA, Sehore
07/02/2015 Dr. N.P. Singh
RAKCA, Sehore
20/02/2015 Dr. A.K. Singh (VC, RVSKVV) and ICARDA Scientists
RAKCA, Sehore
07/03/2015 Dr. H.S. Yadav (DRS, RVSKVV, Gwalior)
RAKCA, Sehore
17/03/2015 Dr. A.K. Tiwari (Director Pulses, Bhopal)
RAKCA, Sehore
22/03/2015 Dr Sanjeev Gupta (PC, MULLARP)
RARS, Nandyal
16/01/2015 Dr. S. Srinivasan and Dr. Baldev Dogra
RARS-Bijapur
Dr Ashutosh Sarker from ICARDA
Pictures of field trials/field visits
ICRISAT-Patancheru
IIPR-Kanpur
Visit of Drs DP Malik, SJ Singh, Rajeev Varshney, PM Gaur and M Yasin at IIPRKanpur
IARI-New Delhi
PAU-Ludhiana
RAKCA-Sehore
RARS-Nandyal
ARS-Bijapur
Acknowledgements
Department of Agriculture & Cooperation,
Ministry of Agriculture, Government of India
Thank you!