Transcript practical dr.arif.ppt

DNA-VIRUSES
1-Poxviridae
2-Herpesviridae
3-Hepadnaviridae
4-Adenoviridae
Poxviridae
1-Brick-shaped
2-Complex structure
3-ds-DNA
Disease : smallpox
Herpesviridae
1-Spherical nucleocapsid
2-Loose envelope
3- ds-DNA
Diseasee :
1-Gingivostomatitis
2-Keratoconjuctivitis
3-Meningo-encephalitis
4-Genital herpes
Hepadnaviridae
1- Three forms of particles
-Spherical HBsAg
particles
-Tubules HBsAg
particles
- HBV-particles
2-ds-DNA enveloped -
Adenoviridae
1- spherical particles
2- Unenveloped
3-ds-DNA
Diseases
1- Pharyngoconjunctival
fever
2-Kerato-conjunctivitis
3- Gasteroenteritis
RNA-VIRUSES
1-Rhabdoviridae
2-Filoviridae
3-Orthomyxoviridae
4-Caliciviridae
5-Reoviridae
Rhabdoviridae
1- Bullet-shaped
2- Helical nucleocapsid
3- ss- RNA
Diseases
1- Acute encephalitis
Filoviridae
1- Filament in shape
2- ssRNA
Diseases
1- Hemorrhagic fevsr
Viruses:
Ebola virus
Marburg virus
Caliciviridae
1- Spherical particles
2- Cup-shaped depression
3- SS- RNA
Diseases
1- Viral diarrhea
2- Hepatitis E
Viruses
Caliciviruses
HEV
Orthomyxoviridae
1- Pleomorphic
2- Covered with spikes
3- Segmented SS-RNS
Dideases
1- Flue
2- URT- infection
3- LRT- infection
eg :
Influenza virus
Reoviridae
1- Double capsid
2- Wheel- structure
3- unenveloped
4-ds-RNA
Diseases :
1- Infantile diarrhea
Viruses
Rotaviruses
Egg inoculation routes
1-Chorio-allantoic membrane
2-Allantoic membrane
3-Amniotic cavity
4-Yolk sac
Chorio-allantoic membrane
HSV-1
Amniotic cavity
Allantoic membrane
Influenza viruses
Herpesviridae 1
Adenoviridae-2
Cytopathic effect ( CPE )
The cellular changes that occur in the infected
cell as a result of viral infection.
Types of CPE
1- Cytocidal effect ;
The infected cells are destroyed , detached
from the tissue culture tube and gaps are
formed .
Examples:
HSV-1&2
CPE
2- The infected cells fuse together to form
multinucleated giant cell ( syncytia
formation ) .
CPE
3- Intranuclear inclusion bodies:
The nucleus is enlarged and appear dark>
Examples:
All DNA viruses.
CPE
Owl eye :
Is intranuclear inclusion bodies. It is a
characteristic feature of CMV-infection.
CPE
4- Intracytoplasmic inclusion bodies:
Dark granules appear in the cytoplasm, as a
result of viral replication.
Examples : all RNA-viruses.
CMV-owels eye
CMV-OWEL EYE
:
Not-infected
:
Not-infected
EBV
:
Giant cell
HSV-1
Complement fixation test & hem
agglutination inhibition test
*Do not differentiate between IgM and IgG
antibodies.
*They measure the total amount of Ab.
*Two sera specimens are needed to each
patient( acute & convalescent ).
CFT & HAI
*Results are recorded in a table as follows:
Acute titer conval. Titer ratio(conval./acute)
Pt-1 40
40
40/40 = 1 immunity.
Pt-2 80
1280 1280/80= 16 recent
infection
Pt-3 -ve
-ve
---- not exposed
Interpretation of results
1- conval-titer / acute-titer 4 recent infect.
2- conval-titer / acute titer
4 immunity
3- negative
not exposed
negative
Reverse passive
hemagglutination
* For Ag detection.
*One blood sample is needed.
*Positive specimen appears as red carpet.
*Results are expressed as positive or negative.