Transcript Thailand-Pitsanulok

Improvement of
Brown Plant Hopper Resistant Rice
in Lower Northern Region of Thailand
Using Mass Aided Selection
Group 5
Objectives
To improve the brown plant hopper
resistant traits of rice, Chinat 1, in the
lower northern of Thailand
Materials and methods
The recipient is Chinat 1
Pedigree
IR13146-158-1/ IR15314-43-2-3-3
X BKN6995-16-1-1-2
IR11738 X IR2061-628-1-6-4-3
bph4
Babawee X IR4432-53
Materials and methods
The donors:
Chr 6
Chr 12
•Abhaya (Ab X KDML) BC4/F6 (540-2-1-8)
•bph4 on Chr. 6*
Bph3
bph4*
•Bph10 on Chr. 12
•Rathu Heenati (Rh X KDML) BC2-F3
•Bph3 on Chr. 6
Bph10
Microsat. Marker tried for Bph 3
Bph3
bph4
Bph10
589
510
101
586
314
179
587
50
277
190
136
260
511
225
313
170
253
309
217
270
235
First set
Second set
Third set
Microsat. Marker tried for Bph 3
Bph3
Chr 6
rm170
589
Bph3
586
587
190
511
170
rm190
rm586
bph4
Heterozygous evident
of
Bph3 SSR flanking
markers
for
CHN1 and Rathu
Microsat. Marker tried for bph 4
Chr 6
bph4
510
rm225
Bph3
rm314
314
50
136
225
253
217
bph4
rm217
rm253
rm50
rm136
Heterozygo
us evident
of
bph4 SSR
flanking
markers
for
CHN1*
X
Abhaya
Microsat. Marker tried for Bph10
Bph10
Chr 12
101
179
rm179
277
rm277
260
313
309
270
235
rm260
Bph10
rm309
rm235
Heterozygous evident
of
Bph10 SSR flanking
markers
for
CHN1 X Abhaya
Bc introgressed line
Scheme
Bc introgressed line
April,2004
Augast,2004
December, 2004
End of program
April, 2005
Positions of genes of interest
Expectation
The elite improved Chinat 1 variety carries many
brown plant hopper resistant genes which provide
higher protection level to brown plant hopper
better and more effective than the original one