Adriana Maggi

DOCENTE DI BIOTECNOLOGIE FARMACOLOGICHE CORSO DI LAUREA SPECIALISTICA IN BIOTECNOLOGIE DEL FARMACO

AA 2011/2012 Lezione 7

LE BASI BIOLOGICHE DELL’ INVECCHIAMENTO Invecchiamento e ambiente Invecchiamento e genetica Regolazione endocrina dell’invecchiamento

Non-Programmed Passive Aging Theories

• • • • Aging is a passive result of an organism’s inability to better resist fundamental deteriorative processes.

Aging serves no purpose, is not an adaptation, is not programmed.

Compatible with traditional evolutionary mechanics theory. Mammals needing more time for development needed a longer life span and

therefore developed better maintenance and repair mechanisms that consequently delayed onset of age-related symptoms and diseases relative to shorter-lived mammals.

Poor fit to many other observations of humans, other mammals, and other organisms particularly those that die suddenly from apparent biological suicide following reproduction rather than from gradual deterioration (e.g. Octopus, salmon)

Programmed Active Aging Theories

• • • • Organisms are purposely designed and genetically programmed to age or otherwise limit life span because the deterioration and life span limitation serves an evolutionary purpose.

Aging is an adaptation, a purposeful design feature resulting from the evolution process.

Aging is the result of a potentially complex active aging mechanism or “life span management system.” The mechanism could sense external conditions in order to adapt life span to local or temporary conditions and could operate by manipulating the maintenance and repair functions.

Aging Theory Status

• • • “Main line” consensus of current gerontologists favors the passive theories. Earlier simple deterioration theories have little current scientific credibility in the biology community while still popular in the human-oriented (physician) community.

Some relatively recent discoveries appear to favor aging-by design theories.

Efforts to explain aging based on traditional mechanics and efforts to explain other discrepancies with alternative mechanics cannot be simultaneously valid. Eventually there will be a unified theory.

“Non-Aging” Species

• Some species have been identified that apparently do not age or have

negligible senescence. Older individuals do not appear to be weaker, less agile, less reproductive, more susceptible to disease, or otherwise less fit than younger animals. (Ages of some wild animals can be determined by annual marks in scales or bones similar to tree rings.)

• Some species with age of oldest recorded specimen:

Rougheye Rockfish 205 Years Lake Sturgeon152 Years

•

Aldabra Tortise152 Years

• Common U.S. Eastern Box Turtle is also long-lived (~100 years).

Non-aging species tend to defeat simple deterioration theories and suggest dramatically longer human life spans are possible.

QUALE APPROCCIO SEGUIRE PER LO STUDIO DELLE BASI MOLCOLARI DELL’INVECCHIAMENTO?

Aging Theories

Planned Obsolescence Theory Telomerase Theory of Aging The Neuroendocrine Theory The Free Radical Theory Mitochondrial Theory of Aging The Membrane Theory of Aging The Hayflick Limit Theory (The cell waste accumulation) Glycosylation Theory of Aging Immune system alterations

MALATTIE GENETICHE ESEMPI DI INVECCHIAMENTO PRECOCE

Progeria

and

Werner Syndrome

• • • Hutchinson-Guilford Progeria, a very rare human genetic disease, accelerates many symptoms of aging including atherosclerotic heart disease. Victims usually die by age 13.

Werner syndrome, another genetic disease, involves acceleration of most symptoms of aging including baldness, hair and skin conditions, heart disease, calcification of blood vessels, some cancers, cataracts, arthritis, diabetes, etc. Victims usually die by age 50.

These conditions suggest aging is centrally controlled such that a single genetic defect could result in proportionally accelerating all of the expressed symptoms. Central control suggests aging-by-design

Il gene LMNA che codifica per le laminine di tipo A è stato caratterizzato nel 1993 e mappato sul cromosoma 1q21.2-q21.3 (Lin and Worman 1993; Wydner et al. 1996). La prima malattia umana causata da mutazioni della laminina A identificata per mezzo di clonaggio posizionale era autosomica dominante e determinava la distrofia muscolare Emery-Dreifuss

Laminopatie

• • • • • • • Autosomal dominant (and rarely recessive) Emery Dreifuss muscular dystrophy Cardiomyopathy dilated 1A Limb-girdle muscular dystrophy type 1B Congenital muscular dystrophy “Heart-hand” syndrome Adipose Tissue Dunnigan-type familial partial lipodystrophy • • • • • • • • • • • Lipoatrophy with diabetes and other features of insulin resistance Atypical lipodystrophy syndromes Mandibuloacral dysplasia Peripheral Nerve Charcot-Marie-Tooth disease type 2B1 Progeria Phenotype Hutchinson-Gilford progeria syndrome Atypical Werner Syndrome Variant progeroid disorders Mandibuloacral Dysplasi

Funzioni delle laminine

Capell and Collins Nature Reviews Genetics 7 , 940–952 (December 2006) | doi:10.1038/nrg1906

Charcot Marie-Tooth Lipodistrofia familiare Displasia mandibulo sacrale progeria

Hutchinson-Gilford progeria syndrome

Una malattia autosomica dominante e sporadica e rara che determina invecchiamento precoce: in genere il paziente muore a 13 anni circa per patologie cardiache La base genetica per molti casi di questa patologia consiste nella mutazione della tripletta GGC della laminina A (LMNA) .

in GGT nel codone 608 Questo determina l’insorgenza di un sito di splicing criptico porta alla sintesi di una proteina con una delezione di 50 aa. La regione deleta ha in se la sequenza riconosciuta da enzimi proteolitici che fanno maturare la Laminina. In mancanza di questa parte della proteina, questa viene carbossifarnesilata e si accumula a livello endocellulare e soprattutto a causa della farnesilazione, nella membrana nucleare.

Invecchiamento e genetica Figure 1. Processing of lamin A in normal and HGPS cells Meshorer E., Gruenbaum Y. J. Cell Biol. 2008:181:9-13

.

La presenza di laminina mutata (

progerin

)altera le funzione della membrana nucleare, permeabilità e la trascrizione genica la sua

The is a mouse model prelamin A is metallopeptidase not of progeria where the mutated.

ZMPSTE24, Instead, the the specific protease that is required to remove the C-terminus of prelamin A, is missing.

INVECCHIAMENTO E PROGERIE

I malati di progerie non mostrano: deficit cognitivi elevati livelli ematici di colesterolo e LDL elevati livelli di prot C reattiva Che generalmente si accompagnano all’invecchiamento

SINDROME DI WERNER

I portatori di mutazioni che dterminano la sindrome di Werner manifestano molto precocemente: cataratta, arteriosclerosi, osteoporosi, diabete mellito di tipo II, tumori di origine mesenchimale.

Generalmente la causa di morte è infarto o tumori.

Sindrome di Werner Una patologia autosomica recessiva

La mutazione genica è a carico della DNA elicasi (cromosoma 8 braccio corto) che accorcia la lunghezza dei telomeri. La malattia si manifesta alla pubertà e i portatori della mutazione vivono fino circa 40 anni di età.

A. Topo I usually found in eukaryotes binds the 3’ end of the broken DNA strand, and removes (+) or (-) supercoils. As replicating DNA moves through the structure, the two parental strands (black) are separated by the helicase, while positive supercoiling is removed by the 3’ topoisomerase.

B. A machine able to separate the daughter molecules at the end of replication is formed by a helicase (red) removing the last turns of parental DNA and a type II topoisomerase (green) untangling the daughter duplexes.

C. Nucleosome disruption

.

The positive supercoiling produced by the translocating helicase H (red) destabilizes the nucleosome, while a topoisomerase T (5’ or 3’ Topo I, or eukaryotic topo II, green) efficiently relaxes the negative supercoiling, reforming the normal duplex behind the helicase.

MAPPA DELLE MUTAZIONI IN PAZIENTI CON MALATTIA DI WERNER

HRDC: Human Hematopoietic Cell Derived Rna Cyclase

Le proteine che interagiscono direttamente con la RecQ elicasi comprendono: Ku70/80, BLM, FEN1, TRF2, p53, polß, polδ, RAD51, RAD52, RAD54B, RPA, POT1, PCNA, PARP1, MRE11, e Cdc5L coinvolte in: Replicazione del DNA, Riparazione del DNA, Ricombinazione Metabolismo dei telomeri

Telomero = una regione di DNA ripetitivo al termine del cromosoma necessario per proteggere la parte terminale del cromosoma stesso nei confronti di enzimi degradativi o fusioni con cromosomi vicini. telos (τέλος) “fine“ merοs (μέρος, root: μερ-) "parte".

Con la replicazione dei cromosomi il terminale dei cromosomi si erode e la parte del telomero si accorcia. Un telomero in una cella giovane puo’ raggiungere le 15000 paia di basi (ogni divisione si raccorcia di 25-200 pb)

double stranded, a G-rich single strand forms a terminal 3' overhang Telomeric repeat-binding factor 1

Telomerase is a ribonucleoprotein polymerase that maintains telmere ends by addition of the telomere repeat TTAGGG

INVECCHIAMENTO E SINDROME DI WERNER

REGOLAZIONE ENDOCRINA DELL’INVECCHIAMENTO

Drosophila

melanogaster

STUDIARE VERMI E INSETTI PER CAPIRE L’UOMO

Coenorabditis

elegans

CICLO VITALE DI C.ELEGANS

L4 L3 adulto

Circa 3 giorni a 22°C

embrioni L1 L2

CICLO VITALE DI C.ELEGANS

adulto L4

MANCANZA DI ALIMENTI STADIO DAUER

embrioni L1

LARVA LARVA DAUER

Studio di processi biologici legati a una maggiore morbidità l’esempio dell’invecchiamento

DAF1 (IGF-R) DAF 7 ( TGF b ligand) DAF 4 (Type II TGF b R) AGE 1 (IP3-K) DAF 3, DAF 5 (SMAD prot) DAF 16* DAF 12 3-keto-cholestenoic acid metabolite DAUER SIR2 (deacetilasi attiva di DAF 16) DAF9 (cytochrome C )

GH Insulin/IGF-1 Insulin/IGF-1 IGF-1 Insulin DAF 2 receptor dFOXO TOR DAF 16/FOXO (adip. Tissue) TOR

LONGEVITY

IGF-1R 1R

LONGEVITY LONGEVITY

germline germline

Invecchiamento e ambiente

EVOLUTION:

LAND OF BIOLOGICAL EQUAL OPPORTUNITIES

“EFFECTOR”

SEXUAL REPRODUCTION NUTRIMENT

“REGULATORS”

AGE

EVOLUTION: LAND OF BIOLOGICAL EQUAL OPPORTUNITIES DEATH: A TOOL INDISPENSABLE TO ENSURE THE CONTINUATION OF THE SPECIE

•

FECUNDITY SHOULD BE DIRECTLY PROPORTIONAL TO NUTRIENT AVAILABILITY, but

•

HIGH NUTRIENT AVAILABILITY, FAVORING FECUNDITY, SHOULD SHORTEN THE LIFE SPAN SEXUAL REPRODUCTION

AGING

AS NECESSITY FOR THE CONTINUATION OF LIFE and AS A MEAN TO GIVE TO EACH INDIVIDUAL EQUAL POSSIBILITIES TO GIVE HIS GENETIC CONTRIBUTION TO THE NEXT GENERATION

Intrinsic program for aging aiming at increasing the fraility of the organism: a biological clock(telomers length, mitochondrial viability; DNA replication errors, loss of immune control and inflammation…) sex-dependent (male fecundity cannot be limited as well as in females) Extrinsic factors Fertility-driven nutrition adaptable environment