Transcript The PHB case using Bacillus megaterium
Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium
Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona Instituto de Biotecnología y Agroindustria, Universidad Nacional de Colombia sede Manizales, Betreuer: Benjamen Stenger Ammar Abdulmughni
Overview
Introduction
Polyhydroxyakanoate (PHA): Structure, Biosynthesis Production
Aim of this subject
Results
Summary
Polyhydroxyakanoate (PHA)
PHAs are linear polyesters Many prokaryotes can synthesize and store PHAs in form of granules (up to 80 % of total bacterial weight) PHAs have similar mechanical properties to conventional plastics They are generally biodegradable
Polyhydroxyakanoate (PHA)
The biosynthesis of PHA : certain deficiency conditions and excess supply of carbon sources The most common/produced form of PHA is polyhydroxybutyrate, PHB Like polypropylene or polyethylene.
PHB
Biosynthesis of PHB
PHB Production
Production costs are heigh: PHB accumulate intercellularly: - Use of a solvent - thermal treatment of biomass, enzymatic digestion and washing with an surfactant a self-disruptive strain.
The lysis system of Bacillus amyloliquefaciens phage was inserted into shuttle vector The expression of a target gene is inhibited by glucose When the glucose concentration approached zero, self-disruption is induced
PHB Production
Production costs are heigh: carbon sources The carbon source could account for 25–45% of the total production costs
Cheaper carbon sources
PHB Production
Agroindustrial wastes are attractive candidates as substrates: low prices and heigh availability solving an environmental problem A variety of microorganisms are able to produce PHB from diverse agroindustrial wastes:
Methylobacterium rhodesianum
(Borman et al., 1999 )
Cupriavidus necator
( Cavalheiro et al., 2009)
E. coli CT1061
(Nikel et al., 2008)
Glycerol is carbon source
Crude glycerol is a co-product in the production of biodiesel Glycerol is an important industrial feedstock : - food, drugs, cosmetics, pharmaceuticals, textile and tobacco industries Glycerol can be used as carbon source in microbiological processes Polyhydroxyakanoate (PHA) production is an interesting biological transformation of glycerol.
PHB Production
Currently, PHB is produced at an industrial scale using Gram negative bacteria lipopolysaccharides (LPS) which co-purify with the PHAs and induce a strong immunogenic reaction
(Valappil et al., 2007) Bacillus megaterium
Aim
Techno-economic analysis of PHB production: - Glycerol transformation into PHB (using
Bacillus megaterium
) - Glycerol or Glucose as substrate - Productivity at different conditions
Results
Different initial concentration of glycerol
Biomass PHB Production The fermentation conditions: temperature 30 °C, air flow 12 l/min and uncontrolled pH
Results
Different temperatures of fermentation
Biomass PHB Production The fermentation conditions: initial glycerol concentration of 20 g/l, air flow 12 l/min, and uncontrolled pH
Results
PHB production using glucose or glycerol
Very Similar yields
Results
Batch cultivation: The fermentations to produce PHB were carried out for 42 h in a 3.7 l Lab Fermen
Results
Economic analysis: The current sale prices are between 3.1 and 4.4 USD/kg
Summary
PHAs are linear polyesters and can be synthesized and itnracellularly stored by many prokaryotes.
The most produced form of PHA is PHB PHAs have similar mechanical properties to conventional plastics Production costs are heigh PHB Production using
B. megaterium,
and glycerol as carbon source The results confirm the ability of B. megaterium to use glycerol as the only carbon source.
References
Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium, Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona, Bioresource Technology 133 (2013) 38–44.
Polyhydroxybutyrate synthesis on biodiesel wastewater using mixed microbial consortia, Zachary T. Dobroth a, Shengjun Hub, Erik R. Coats a, Armando G. McDonaldBioresource Technology 102 (2011) 3352–3359 Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV, Valappil SP, Misra SK, Boccaccini AR, Keshavarz T, Bucke C, Roy I., J Biotechnol. 2007 Nov 1;132(3):251-8 Construction of self-disruptive Bacillus megaterium in response to substrate exhaustion for polyhydroxybutyrate production. Hori K, Kaneko M,TanjiY, Xing XH, Unno H. Graduate School of Bioscience and Biotechnology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.