Transcript RNA중합효소가 특이적으로 결합하는 부위 시그마 소단위체 역할은

Bubble length : 12-14 bp
RNA-DNA hybrid : 8-9 bp
The overall reaction rate is
- 40 nucleotides/second at 37oC
Whereas
Rate of DNA replication is 800 bp / sec.
Core enzyme binds indiscriminately to any DNA.
Sigma factor reduces the affinity for sequence –
independent binding,
confers specificity for promoters
A closed binary complex is
converted to an open form
and
then into a ternary complex
Enhancer
Promoter : RNA중합효소가
특이적으로 결합하는 부위
시그마 소단위체 역할은
CPC - OPC로 전환
전사개시에 필수
Core enzyme
Holoenzyme