Transcript IMMUNOPHENOTYPING LEUKEMIAS AND LYMPHOMAS RPCI

IMMUNOPHENOTYPING
LEUKEMIAS AND
LYMPHOMAS
RPCI
LFC
CD45 Gating Strategy
NORMAL BONE MARROW
A
0
256
SSC ->
/6/05133061
512
768
LEUKEMIC (TALL) BONE MARROW
B
1024
10 0
101
HLADr ->
/6/05133061
granulocytes
10 2
10 3
C
10 4
0
256
SSC ->
/7/06064121
512
768
D
1024
10 0
10 1
HLADr ->
/7/06064121
erythrocytes and
platelets
monocytes and B-cells
10 2
10 3
10 4
CD45 EXPRESSION BY ACUTE B-LINEAGE LEUKEMIA
CD45
A
HLADr
CD45
B
HLADr
CD45
C
HLADr
B LINEAGE ACUTE LEUKEMIA
C
D
1
4
C
D
1
4
C
D
4
5
CD45
HLADr
4
10
4
10
4
10
1
HLADr
2
5
CD45
3
10
1
FL1 -->
10
10
10 2
2
1
10
1
4
10
R5
C
D
4
5
7
4
10
1
10
2
HLADr
FL3 -->
10
3
10
4
6
3
10
10
C
D
1
4
FL1 -->
2
1
10
10
3
3
2
FL2 -->
10
FL2 -->
C
D
1
4
10
3
10
R1
8
10
1
10
2
10
FL3 -->
HLADr
3
10
4
4
10
10
1
10
2
10
3
10
4
1
10
1
CD5
10
2
10
3
10
10
4
1
10
10
CD5
3
2
FL1 -->
10
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
2
3
10
1
10
2
FL3 -->
CD10
4
6
R5
C
D
5
7
4
10
3
10
3
10
3
10
C
D
1
9
4
10
10
4
10
4
10
4
10
FL2 -->
3
2
5
R1
C
D
1
9
10
CD10
2
1
1
CD10
1
10
10
1
10
10
2
10
C
D
5
2
3
3
10
10
4
4
10
3
2
10
C
D
1
9
1
C
D
1
9
10
B LINEAGE ACUTE LEUKEMIA
10
3
10
4
8
10
1
10
2
FL3 -->
CD10
10
3
10
4
10
2
10
3
10
4
10
10
1
1
10
2
10
3
10
4
10
10
1
10
2
CD5
10
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
10
FL1 -->
3
10
1
10
2
FL3 -->
CD19
4
6
R5
C
D
5
7
4
10
3
10
3
10
3
10
C
D
3
4
4
3
10
4
10
4
10
4
10
FL2 -->
3
2
5
R1
C
D
3
4
10
CD19
2
10
1
CD19
1
2
2
10
10
CD5
1
10
3
10
4
10
4
10
3
1
2
2
10
1
10
C
D
5
10
C
D
3
4
10
C
D
3
4
1
3
10
4
10
B LINEAGE ACUTE LEUKEMIA
10
3
10
4
8
10
1
10
2
FL3 -->
CD19
10
3
10
4
10
1
10
2
10
3
10
10
4
10
1
CD20
10
2
10
3
10
2
10
1
CD22
10
10
FL1 -->
3
10
3
R5
10
1
10
2
FL3 -->
CD22
10
4
3
10
4
6
C
D
2
0
7
4
10
3
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
C
D
1
1
c
4
10
2
4
10
3
10
3
10
3
10
CD22
4
10
4
10
FL2 -->
C
D
1
1
c
2
1
5
R1
10
2
10
2
1
10
4
CD22
1
10
10
3
10
4
1
2
10
C
D
2
0
10
10
2
10
C
D
1
1
c
1
C
D
1
1
c
1
3
3
10
10
4
4
10
10
B LINEAGE ACUTE LEUKEMIA
8
10
1
10
2
FL3 -->
10
CD22
3
10
4
4
4
4
10
10
10
B LINEAGE ACUTE LEUKEMIA
5
2
10
1
10
2
10
1
2
10
1
C
D
3
3
10
3
10
3
10
3
C
D
1
3
10
C
D
1
3
10
1
7
3
10
1
10
2
10
3
10
10
4
CD33
1
10
2
10
3
10
10
4
CD19
1
10
10
CD33
3
2
FL1 -->
10
10
10 2
6
R5
C
D
3
3
7
4
10
1
10
2
FL3 -->
CD19
4
3
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
FL2 -->
C
D
1
3
4
1
10
4
10
3
10
3
10
R1
3
10
3
5
2
C
D
1
3
10
2
CD19
4
10
4
10
1
10
8
1
10
3
10
4
8
10
1
10
2
FL3 -->
CD19
10
3
10
4
C
D
1
9
C
D
2
0
CD10
CD22
C
D
4
5
C
D
3
4
CD19
HLADr
T LINEAGE ACUTE LEUKEMIA
C
D
1
4
C
D
4
5
C
D
1
4
CD45
HLADr
4
10
4
10
4
10
1
HLADr
2
5
FL1 -->
CD45
10
3
10
1
10 2
10
1
2
4
10
C
D
4
5
10
C
D
1
4
7
4
10
1
10
2
FL3 -->
HLADr
10
3
10
4
6
3
10
1
FL1 -->
10
1
2
10
3
10
2
FL2 -->
10
FL2 -->
C
D
1
4
3
10
3
10
R1
R5
8
10
1
10
2
HLADr
FL3 -->
10
3
10
4
3
10
10
2
10
3
10
10
1
10
2
10
3
10
4
10
CD8
1
10
10
CD3
10
2
10
FL1-Height -->
10
1
FL1-Height -->
10
1
2
10
10
2
FL2-Height -->
10
FL2-Height -->
C
D
4
10
4
10
1
10
2
FL3-Height -->
CD8
10
3
10
4
10
R5
4
6
C
D
3
7
4
3
10
3
10
3
10
3
10
R1
3
CD8
FL3 -->
3
5
2
C
D
4
2
4
10
4
10
4
10
1
10
8
1
FL3 -->
CD3
2
FL1 -->
3
7
4
FL1 -->
1
10
10
1
4
1
2
3
10
2
10
C
D
3
1
10
2
10
C
D
4
1
C
D
4
10
5
10
2
3
10
R1
4
10
4
1
10
4
10
T LINEAGE ACUTE LEUKEMIA
8
10
1
10
2
FL3-Height -->
CD8
10
3
10
4
1
10
2
10
3
10
4
10
4
10
3
10
1
CD7
10
2
10
3
10
4
10
1
10
10
CD7
10
2
10
FL1 -->
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
10
3
R5
10
1
10
2
FL3 -->
CD2
4
10
3
10
4
6
C
D
7
7
4
10
3
10
3
10
3
10
FL2 -->
C
D
1
9
4
3
10
4
10
4
10
4
10
R1
3
2
5
2
C
D
1
9
10
FL3 -->
CD2
FL3 -->
1
2
1
CD2
FL1 -->
1
10
10
1
1
10
2
2
10
C
D
7
10
10
2
10
C
D
1
9
1
C
D
1
9
3
3
10
10
4
4
10
10
T LINEAGE ACUTE LEUKEMIA
8
10
1
10
2
CD2
FL3 -->
10
3
10
4
4
10
10
1
10
2
10
3
10
4
1
10
1
10
CD5
2
10
3
10
10
4
1
10
10
3
2
CD5
FL1 -->
10
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
C
D
1
9
10
R5
4
10
1
10
2
CD10
FL3 -->
4
10
3
10
4
6
C
D
5
7
4
2
3
3
10
3
10
3
10
3
10
4
10
4
10
4
10
FL2 -->
C
D
1
9
2
5
R1
10
10
CD10
2
1
1
CD10
1
10
10
1
10
10
2
10
C
D
5
2
3
3
10
10
4
4
10
3
2
10
C
D
1
9
1
C
D
1
9
10
T LINEAGE ACUTE LEUKEMIA
8
10
1
10
2
CD10
FL3 -->
10
3
10
4
1
10
2
10
3
10
4
10
10
1
1
10
2
10
3
10
4
10
10
1
10
2
CD5
10
10
1
FL1 -->
10
1
2
10
10
2
FL2 -->
10
10
FL1 -->
3
10
1
10
2
FL3 -->
CD19
4
6
R5
C
D
5
7
4
10
3
10
3
10
3
10
C
D
3
4
4
3
10
4
10
4
10
4
10
FL2 -->
3
2
5
R1
C
D
3
4
10
CD19
2
10
1
CD19
1
2
2
10
2
10
CD5
1
10
3
10
4
10
4
10
3
10
C
D
5
10
10
2
10
C
D
3
4
1
C
D
3
4
1
3
10
4
10
T LINEAGE ACUTE LEUKEMIA
10
3
10
4
8
10
1
10
2
FL3 -->
CD19
10
3
10
4
10
1
10
2
10
3
10
10
4
10
1
CD45
10
2
10
3
10
2
10
1
CD45
10
10
FL1-Height -->
3
10
3
R5
10
1
10
2
FL3-Height -->
HLADr
4
10
3
10
4
6
C
D
4
5
7
4
10
3
10
1
FL1-Height -->
10
1
2
10
10
2
FL2-Height -->
10
C
D
1
4
4
10
2
4
10
3
10
3
10
3
10
HLADr
4
10
4
10
FL2-Height -->
C
D
1
4
2
1
5
R1
10
2
10
2
1
10
4
HLADr
1
10
10
3
10
4
1
2
10
C
D
4
5
10
10
2
10
C
D
1
4
1
C
D
1
4
1
3
3
10
10
4
4
10
10
ACUTE MYELOID LEUKEMIA
8
10
1
10
2
10
HLADr
FL3-Height -->
3
10
4
10 2
10 3
10 4
10
10 3
CD11b
FL1-Height -->
10 3
10 4
10
2
CD33
6
R5
C
D
1
1
b
1
10
10 2
7
10 1
10 2
CD33
FL3-Height -->
10 3
10 4
10 4
2
1
10
10
FL1-Height -->
2
1
10
10
C
D
1
3
4
10 1
10 3
3
10
3
2
FL2-Height -->
10
3
10 2
4
10
CD33
10 1
5
10
3
10
FL2-Height -->
C
D
1
3
10
10 4
2
R1
1
10
10 2
4
1
2
10 1
10
4
10
CD11b
3
10
4
10
4
10
3
10 1
10
10
2
10
C
D
1
3
1
C
D
1
3
C
D
1
1
b
1
3
10
4
10
ACUTE MYELOID LEUKEMIA
8
10 1
10 2
CD33
FL3-Height -->
10 3
10 4
4
10
1
4
10
4
10
ACUTE MYELOID LEUKEMIA
2
5
10
2
10
1
1
C
D
1
5
4
10
1
10
2
10
3
10
7
10
4
1
10
2
10
3
10
4
CD15
10
1
10
10
CD15
3
2
FL1-Height -->
10
10
1
FL1-Height -->
10
1
2
10
10
2
FL2-Height -->
10
2
R5
10
1
10
2
FL3-Height -->
CD56
10
3
10
4
6
C
D
1
5
7
4
10
3
10
3
10
3
10
10
3
4
10
4
10
4
10
FL2-Height -->
C
D
3
4
4
1
10
5
R1
3
10
2
CD56
2
C
D
3
4
1
FL3 -->
CD56
1
10
8
FL3 -->
FL1 -->
6
3
10
10
C
D
3
4
FL1 -->
2
1
10
10
3
3
2
FL2 -->
10
FL2 -->
C
D
3
4
10
3
10
R1
R5
8
10
1
10
2
FL3-Height -->
CD56
10
3
10
4
4
10 2
10 3
10 4
10
10 3
10 4
10 1
5
2
10
1
CD7
10 4
10
4
10 3
R5
10 2
FL3-Height -->
CD2
10 3
10 4
6
C
D
7
7
10 1
10 4
3
10
1
C
D
1
3
FL1-Height -->
10
1
2
10
10
2
FL2-Height -->
10
3
3
10
3
10
FL2-Height -->
C
D
1
3
10 3
4
10
4
10
4
10
R1
10 2
CD2
2
FL1-Height -->
2
1
10 2
CD2
1
10 2
10
10
2
10 1
CD7
10 1
10
3
10
4
10
4
10
3
10 1
C
D
7
10
10
2
10
C
D
1
3
1
C
D
1
3
1
3
10
4
10
ACUTE MYELOID LEUKEMIA
8
10 1
10 2
FL3-Height -->
CD2
10 3
10 4
10
1
10
2
10
3
10
4
10
4
10
3
2
10
C
D
3
8
10
10
2
10
C
D
3
4
1
C
D
3
4
1
3
10
4
10
ACUTE MYELOID LEUKEMIA
8
10
1
10
2
10
3
10
4
10
3
10
4
10
1
10
10
CD38
10
2
10
FL1-Height -->
3
10
1
FL1-Height -->
10
1
2
10
10
2
FL2-Height -->
10
10
2
10 4
R5
C
D
3
8
7
4
10
1
10
2
FL3-Height -->
HLADr
10
3
10
4
6
3
10
3
10
3
10
FL2-Height -->
C
D
3
4
4
1
10
3
5
R1
3
10
2
HLADr
2
C
D
3
4
1
4
10
1
10
2
HLADr
4
10
4
10
CD38
10
1
8
10
1
10
2
FL3-Height -->
HLADr
10
3
10 4
4
10
1
2
A
10
3
C
D
1
9
4
B
5
6
C
D
2
0
10
2
10
FL2-Hei ght -->
1
R1
10
2
10
R5
FL1-Hei ght -->
4
101
102
103
7
104
D
3
1
10
2
10
3
2
10
4
101
4
10
Fluorescence Two Height -->
3
4
103
Fluorescence
One Height -->
CD5
104
E
5
6
2
10
4
10
L
A
M
B
D
A
10
7
8
103
R1
Fluorescence Three Height -->
Fluorescence Two Height -->
104
2
10
10
Fluorescence One Height -->
F
1
2
1
CD20
104
10
10
102
103
3
1
101
102
KAPPA
R5
R1
10
102
3
104
FL1-Hei ght -->
3
C
D
2
2
10
1
R1
CD22
2
10
10
FL3-Hei ght -->
1
1
2
8
10
CD5
C
D
1
9
10
10
FL1-Hei ght -->
10
3
1
10
3
C2
1
FL2-Hei ght -->
1
10
4
10
L
A
M
B
D
A
3
4
3
101
102
4
10
3
Fluorescence One Height -->
KAPPA
Typical B-cell Lymphoma
104
4
4
10
A
10
C
D
1
9FL2-Height -->
1
B
C
D
1
9
3
1
10
10
R1
3
102
103
104
101
FL3-Height -->
10
C
D
1
9
4
10
C
D
1
9
3
10
2
10
102
F
104
R1
101
10
C
D
1
9
3
103
4
2
Fluorescence Two Height -->
Fluorescence Three Height -->
CD2
101
1 02
103
CD7
103
104
101
102
103
104
CD19 -->
CD19
G
4
10
H
C
D
1
3CD13 -->
3
3
10
10
2
2
10
10
1
1
10
10
Fluorescence Two Height -->
4
Fluorescence One Height -->
1 02
CD10
10
10
101
10
FL3-Height -->
2
1
Fluorescence Two Height -->
1
10
1
104
10
10
2
4
103
3
1
10
10
CD7
E
3
1
Fluorescence One Height -->
CD2
4
102
D
C
D
1
3CD13 -->
10
Fluorescence Two Height -->
101
10
2
10
1
C
10
2
10
4
3
10
2
10
C
D
1
9FL2-Height -->
3
10
4
2
1 04
101
102
103
1 04
1 01
Fluorescence Three Height -->
CD10
T, CD10, and Myeloid Antigen Coexpression
in B-cell lymphoma
102
CD19 -->
CD19
1 03
104
4
10
C
D
4
B
4
A2
1
10
4
10
3
3
3
10
10
10
C
D
4
2
10
L AMB D A -->
C
D
7
2
10
2
10
K A PPA -->
L AMB D A -->
R1
1
1
1
10
10
10
3
4
10
1
10
2
10
3
6
C
5
R5
7
10
4
10
1
K A PPA -->
10
2
10
3
4
10
8
1
10
2
10
3
10
C D 19 CD2
-->
CCD8
D 19 -->
CD3
10
Lymphoma
4
10
C
D
4
D
2
1
4
C
D
4
3
10
4
E
10
10
C
D
7
3
10
10
2
2
10
10
10
KAPPA -->
LAM BDA -->
R1
1
1
1
10
10
10
3
4
101
102
KAPPA -->
CD3
10
3
104
6
F
3
2
LAM BDA -->
5
R5
7
101
102
CD19
-->
CD8
103
10
4
8
101
10
2
103
CD19
-->
CD2
Re activ e Lymp h No de
Typical T-cell Lymphoma or Reactive Lymph Node:
104
4
A
L
a
m
b
d
a
B
L
a
m
b
d
a
Kappa
C
D
1
3
K
a
p
p
a
CD19
D
CD19
E
C
D
1
3
CD33
C
C
D
3
3
CD19
Problem Case
CD19
F