Transcript Document 7726585

EXPERIMENT:
Action of Amylase on Starch
Add 10 ml of distilled water to each tube.
A
B
C
D
E
F
G
Add 7 drops of iodine solution to each tube.
A
B
C
D
E
F
G
Add 1 ml of 1% soluble starch solution to tube A.
A
B
***Starch + iodine =
C
D
E
F
G
Place 10 ml 1% soluble starch solution in a 50 ml beaker and
add 2 ml of 2% amylase solution, stirring as you add.
Start timing after you finish adding the amylase.
amylase
starch
After 2 minutes, add 1 ml of the starch-amylase solution
to tube B.
A
B
C
D
E
F
G
After 2 more minutes (4 minutes from time zero), add 1 ml of
starch-amylase solution to tube C.
A
B
C
D
E
F
G
Repeat this process every 2 minutes for the rest of the tubes.
A
B
C
D
E
F
G
Observe the colors and record your observations.
A
B
C
D
E
F
G
Add remaining starch-amylase solution into test tube 1.
1
Add an equal amount of original 1% soluble starch solution
into test tube 2.
1
2
Add 2 ml of Benedict’s solution to each tube.
1
2
Heat in water bath for 3 to 5 minutes.
1
2
Observe color change . Orange = sugar.
1
2