Document 7611904

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BBSI Research Simulation
Overexpression of Protein
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Transcriptional vs Translational Fusions
your gene
5’-GTGAGTTAGCTCACNNNNNNNNNNTANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNN
3’-CACTCAATCGAGTGNNNNNNNNNNATNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNTACNNNNNNNNNNNNNNNN
Operator
RNA Polymerase
Taken from lacZ regulatory region
Transcription
Translation
Taken from
source of gene
Transcriptional vs Translational Fusions
lacZ
Operator
RNA Polymerase
Transcription
Translation
Transcriptional Fusions: How do they help (SQ1)
your gene
5’-GTGAGTTAGCTCACNNNNNNNNNNTANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNN
3’-CACTCAATCGAGTGNNNNNNNNNNATNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNTACNNNNNNNNNNNNNNNN
Operator
RNA Polymerase
Transcription
Translation
Death
Transcriptional Fusions: How do they help (SQ1)
Repressor
your gene
5’-GTGAGTTAGCTCACNNNNNNNNNNTANNNTNNNNNNNNNNNNNNNNNNNNNNNNNNNNATGNNNNNNNNNNNNNNNN
3’-CACTCAATCGAGTGNNNNNNNNNNATNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNTACNNNNNNNNNNNNNNNN
Operator
RNA Polymerase
Transcription
LIFE
Translation
Death