Transcript Metabolic network architecture and carbon source determine

Supporting information
Metabolic network architecture and carbon source determine metabolite
production costs
Silvio Waschina, Glen D'Souza, Christian Kost, and Christoph Kaleta
Table S1. Strains used in this study.
Strain
Genotype
Phenotype
Reference
Escherichia coli
F-, ΔaraD-araB567,
WT
[1]
BW25113 ara-
ΔlacZ4787::rrnB-3, λ-, rph-1,
ΔrhaD-rhaB568, hsdR514
∆hisD
WT ara-, ∆hisD::kanR
AT
[2]
∆pheA
WT ara-, ∆pheA::kanR
AT
[2]
∆tyrA
WT ara-, ∆tyrA::kanR
AT
[2]
∆trpB
WT ara-, ∆trpB::kanR
AT
[2]
∆leuB
WT ara-, ∆leuB::kanR
AT
[2]
∆lysA
WT ara-, ∆lysA::kanR
AT
[2]
∆ilvA
WT ara-, ∆ilvA::kanR
AT
[2]
∆hisD
WT ara-, ∆hisD
AT, kanS
This study
∆pheA
WT ara-, ∆pheA
AT, kanS
This study
∆trpB
WT ara-, ∆trpB
AT, kanS
This study
∆leuB
WT ara-, ∆leuB
AT, kanS
This study
∆lysA
WT ara-, ∆lysA
AT, kanS
This study
∆ilvA
WT ara-, ∆ilvA
AT, kanS
This study
Abbreviations: ara- – inability to use arabinose as carbon source, WT – wild type, AT – auxotroph, kanS – kanamycin
sensitive.
References
1 Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner
BL & Mori H (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout
mutants: the Keio collection. Mol. Syst. Biol. 2, 2006.0008.
2 D’Souza G, Waschina S, Pande S, Bohl K, Kaleta C & Kost C (2014) Less is more: selective
advantages can explain the prevalent loss of biosynthetic genes in bacteria. Evolution 68,
2559–2570.