Transcript LC-MS: Ascorbic acid related compounds

No.TI785E
LC-MS: Ascorbic acid related compounds
uM
1
O
2 3
O
H
OH
1
C
HOCH2
4
O
O
dehydroascorbic acid
100
H
2
HOCH2
0.0
H
3
C
HOCH2
4
2
uM
O
O
OH
Scherzo SM-C18, 150 x 3 mm
A: 5 mM AcONH4
[M-H]- m/z 175.1
B: ACN / water / AcOH = 30 / 70 / 0.1
70-80 %B (0-10 min)
[M-H]- m/z 173.1
0.4 mL/min (10 MPa), 37°C
10 uL (100 nmoL/mL)
2.0
4.0
6.0
8.0
10.0 min Single Quad. MS: ESI (SIM,negative)
0
OH
O
HO
isoascorbic acid
3
1
100
4
O
OH
CH2OH
O HO
OH
HO
[M-H]m/z 337.1
50
[M-H]- m/z 175.1
[M+HCOO]- m/z 219.0
0
0.0
C
HO
L-ascorbic acid
[M-H]m/z 337.1
50
OH
2.5
5.0
7.5
10.0
Imtakt USA ([email protected])
North America
12.5
O
OH
H CH2OH
C
OH
O
O
L-ascorbic acid 2-glucoside
Scherzo SM-C18, 150 x 3 mm
A: ACN / HCOOH = 100 / 0.1
B: water / HCOOH = 100 / 0.3
0-50 %B (0-15 min)
0.4 mL/min (4 MPa), 37°C
10 uL (100 nmoL/mL)
15.0 min Single Quad. MS: ESI (SIM,negative)
Imtakt Corp./JAPAN ([email protected])
Other Countries
TI785E-OB10