Transcript Cleaning and disinfection of large and small areas

Cleaning and disinfection of large and small areas
basan initiates cooperation study for the purpose of proving
microbiological process reliability
The innovative design of the new EasyMop GMP® systembox makes it possible both to
perform the preparation of several mop covers in the system, and also to leave the
equipment in the cleanroom for several days. The advantages are plain to see: greater
operator safety, efficiency and economic viability in the cleaning and disinfection
process. However, isn't there a danger in this, the danger of systemimmanent harmful
microbiological material? basan – the cleanroom division of VWR International GmbH
addressed this issue in the cooperation study.
The foundation for efficient cleaning and disinfection is easy-to-handle, safe and efficient
equipment. Intelligent design, as offered by the new EasyMop GMP® systembox, leads to a
minimisation of human sources of error in the wiping process, making this process safe,
efficient and economically viable. The system is also space-saving and can be kept in the
monitored area for a longer period – an aspect particularly relevant to smaller production
sites, of which there are many in the dispensing chemists' sector.
A particular advantage of the innovative EasyMop GMP® systembox is the opportunity to
perform the preparation for several mop covers in the closed system and also the opportunity
to leave the whole system in the clean room. This avoids important sources of error – the
requirement is that this process does not in itself present the relevant location with a
microbiological burden and also does not cause the disinfectant's effectiveness to be
diminished.
In order to examine these issues, basan, the cleanroom division of VWR International GmbH,
initiated a cooperation study with the companies Diversey and Pfennig Reinigungstechnik
GmbH. The aim in this was to prove that no risk of an additional contamination emerges with
the new EasyMop GMP® systembox. The intention was also to show that lengthening the
standing time does not influence the disinfecting effect of the solution used, and that the
active-substance content remains sufficiently high to be able to prevent micro-organic growth
in the solution.
Doing the groundwork for the study:
10 sterile mop covers were placed into the EasyMop© GMP box including the MopJump, in
accordance with instructions issued, and soaked with 4 litres of sterile disinfectant
(ClearKlens Tego 2000 RTU). From Tuesday to Monday (extending the standing time to 7
days), two mop covers respectively were removed and wrung out. In this wrung-out water,
the amphotere tenside content was precisely calculated and the total germ quantity was
determined by microbiological means. Using a funnel, each of the mop covers was wrung out
individually into a re-sealable glass. The respective concentration level of the disinfectant
was calculated by using this wrung-out water. Another millilitre of wrung-out water was
distributed out onto a single-use petri dish with CASO agar and incubated for 48 hours.
The results:
The starting content of the ready-to-use disinfectant solution equalled 1.01 per cent. For both
mop covers, the amphotere tenside content in the solution after 10 minutes (Day 1) still
registered a value of 0.86 per cent, i.e. part of the active substance is absorbed by the fibres.
During the study, the active substance decomposed on an even basis. After the 7th day
(Tuesday to Monday), the readings recorded 0.77 per cent and 0.78 per cent respectively.
The outcome from the assessment of the nutrient media, spanning the study's whole period,
was that no cultures had grown on the nutrient media with the wrung-out water that had been
placed on the Petri dish.
Conclusion:
The study proves convincingly that the EasyMop GMP® systembox is a safe system, not
entailing any system-immanent risks for microbiological contaminations. The active
substance's loss of effectiveness over the proposed standing time of five days is within the
customary range. If it remains where it is for a longer period, this does not lead to a greater
loss. Thus, in large and small cleanrooms, the intelligent design offers the user possibilities
for cleaning and disinfection in a way that is simple, efficient, safe, space-saving and, as a
result, economically viable.
Prospect:
Each production location requires an individual concept for safe cleaning and disinfection.
This concept must bear in mind the people conducting this process, analyse the situation in
terms of the spaces to be worked with, and also keep economic viability front of mind as a
factor. The use of flexible, certified systems, suitable both for small and for large areas, with
an individual selection of highly-effective cleaning agents and disinfectants, and with welltrained staff, matches the current and future requirements generated by cleaning and
disinfecting in controlled areas.
VWR International GmbH basan - the cleanroom division of VWR • Distribution Center Kelsterbach • Donaustrasse 1 •
65451 Kelsterbach, Germany • Tel.: + 49 6107/9008-500 • Fax: + 49 6107/9008-809 • [email protected]
www.basan.com
© 2014 VWR International GmbH basan - the cleanroom division of VWR