Reproduction of the Barnacle Balanus glandula Erin Breck Oregon State University Advisor Dr. Bruce Menge Partnership for Interdisciplinary Studies of Coastal Oceans (PISCO)
Download ReportTranscript Reproduction of the Barnacle Balanus glandula Erin Breck Oregon State University Advisor Dr. Bruce Menge Partnership for Interdisciplinary Studies of Coastal Oceans (PISCO)
Reproduction of the Barnacle Balanus glandula Erin Breck Oregon State University Advisor Dr. Bruce Menge Partnership for Interdisciplinary Studies of Coastal Oceans (PISCO) Outline Background Hypotheses Methods Results Life History Free swimming larvae, 100-300 um long Cyprid, 500 um length Adult, 5-10 mm diameter Significance Contribute knowledge of early life history Model for marine population dynamics Oregon Coast Study Sites 45o Fogarty Creek Newport Bob Creek 44o 43o Cape Blanco Hypotheses 1) The number of embryos is positively correlated with barnacle size. 2) Embryo size increases with barnacle size. 3) Barnacles at the southern site produce more embryos than those at the northern site. Methods Collect Embryos Prepare Samples Count Measure The number of embryos is positively correlated with barnacle size. Mean Embryos per Barnacle P-value < 0.0001 Number of embryos R2 = 0.558 6000 5000 4000 3000 2000 1000 0 0 0.05 0.1 0.15 0.2 Volume of Barnacle (cm3) 0.25 0.3 Embryo size is slightly correlated with barnacle size. Size of Embryos for a Barnacle P-value = 0.0126 Embryo Size (um) R2 = 0.4172 1 0.8 0.6 0.4 0.2 0 0 0.05 0.1 0.15 Volume of Barnacle (cm3) 0.2 0.25 Barnacles at the southern site produce more embryos than those at the northern site. Mean Embryos per Site Number of Embryos P- value = 0.0188 1600 1400 1200 1000 800 600 400 200 0 BC FC Site Summary 1) The number of embryos is positively correlated with barnacle size. 2) Embryo size is slightly correlated with barnacle size. 3) Barnacles at the southern site produce more embryos than those at the northern site. Implications 1) Evolutionary constraints 2) Documented a significant difference in reproduction between north and south Determine condition of barnacle larvae Measure Lipids Method: Stain with dye Use fluorescent microscope Photograph Computer Analysis Predicted Results: South versus north sites 50 um Next Steps More Counting Data Analysis Acknowledgements Howard Hughes Medical Institute Brian Breck Francis Chan Kaitlin Fraser Helen Harker Heather Leslie Dr. Jane Lubchenco Dr. Bruce Menge Ruth Milston Alana Nicovich Mae Noble Shannon Oda Dr. Barbara Taylor The Lubmengo crew