Transcript Oral Presentation for Chem 201: Title of Lab Goes Here U.R. Name Lab Partner: T.H.

Oral Presentation for Chem 201:
Title of Lab Goes Here
U.R. Name
Lab Partner: T.H. Guy
Presentation date: 11/7/2015
11/7/2015
Slide 1
Introduction
 The INTRODUCTION introduces the talk
 Include in your Introduction
 The problem you addressed
 Your experimental approach to the problem
 The significance of your work
 Other ground rules:
 Bullet points are best

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
Don't use too many colors
Don't put too much material on one "slide"




Bullet points should be just that
Say full sentences; don't write them
Use more slides than less slides
Make sure that everything you present is legible.
Use 14-point type or bigger
Leave white space as a border around every slide!
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Slide 2
Details of the Experiment
 Because you can only put one topic/thought per slide, you will
need several slides to fill in critical details.
 The slides that come after the Introduction will be the equivalent
of the Experimental section

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
Provide any theory required that is critical to the calculations and
results you will obtain
Provide an overview of the instrumentation used and any details
about instrumentation that are critical to the data you obtained
Describe the experiments you have performed
Present the critical equations for your calculations of data
Present any other critical details
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Slide 3
Details – Cont.
 State logically (in stepwise fashion) the experimental details and
what you did
 Give big strokes—not tedious details. Examples:
 Prepared solutions of dimuglioglugtane (DMGG) at concentrations of
0.01, 0.1, and 1 M in CDCl3.
 Acquired 1H-NMR spectra

If the number of spectra or other acquisition parameters are important,
include as sub-points.
 Tell how you transformed the raw data into meaningful results
 Make sure you keep everyone focused on the purpose of the
experiment
 Discuss the importance of your findings after you present your
results.
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Slide 4
Other Points
 Cite sources of information in your presentation. Including:
 Articles used in your "overview" etc.
 All figures that you present that are not your own
 Sources of information
 Citations are best placed at the bottom of the slide
 Pictures are always better than words
 Present the pictures; say the words
 Data tables:


Avoid where possible. Use bar graphs, etc. instead of data tables
Keep the tables simple and small
Here is a good place to put any citations you need on any page.
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Slide 5
Example of Introduction Using Both Graphical Info and Bullet Points:
Sulfur Amino Acids (SAA)
 Methionine
O
S
HO
CH3
NH 2
Methionine (Met)
O
SH
HO
NH 2
Cysteine (Cys)

An essential amino acid
 Cysteine

Nonessential amino acid

Synthesis dependent methionine
 Homocysteine

Produced from methionine metabolism

Contributes S for cysteine synthesis
 Elevated homocysteine levels correlate
with cardiovascular disease risk
O
SH
HO
NH 2
Homocysteine
(Hcy)
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Slide 6
Example of an Experimental Procedure in Graphical Terms:
Method to Reduce and Modify
Protein-bound Homocysteine for Measurement by GCMS
DMH
O
Homocysteine Bound to Protein
via a Disulfide Bridge
H3C
O
S
S
H3C
SH
N
N
O
OH
O
SH
O
SH
1
NH2
H3C
OH +
H3C
NH2
N
S
N
S
Homocysteine
2
O
S
N
tBDMS
4-vinyl
pyridine
N
O
MTBSTFA
S
OtBDMS
NH
O
3
N
OH
NH2
S-4-ethyl pyridine bis-tBDMS homocysteine
From MacCoss et al., Anal. Chem. 1999
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Slide 7
Graphical Example of Results:
EI & NCI Mass Spectra of Leucine as the HFBPr Derivative
Abundance
282
69
7000
+
[M-COOPr]+
CF3+
HFB-NH2+ ?
6000
[M-COOPr]
240
EI
5000
4000
O
C3F7
[M-C4H8]+?
3000
313
O
2000
HN
C
O
C3H7
1000
55
0
60
114 131
80
100 120 140
253
214
169
266
160 180 200 220 240 260 280 300 320
m/z
[M-HF]-
Abundance
MW = 369
NCI
25000
?
178
20000
HFB = heptafluorobutyryl
Pr = n-propyl ester
349
15000
10000
5000
160
0
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198
243
281
[M-H]311
368
452
160 180 200 220 240 260 280 300 320 340 360 380 400 420 440 460
m/z
Slide 8
Presenting Results & Discussion
 Don't give ALL the data and ALL the results you have obtained
 Determine what you believe are the key results, and present
them
 Organize your talk around the Discussion

Figure out what results are required to support the points you want
to discuss. Those are the results that you need to present.
 Some data may need to be presented just to confirm that you
correctly collected the results that you think you collected
Examples:


Data that demonstrate the instrumentation being used is working
Data that demonstrate the experimental conditions are being met
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Slide 9
Graphical Example of an Almost Too Busy Table:
Amino Acid Composition of Select Protein Sources
Hen
Egg
Amino Acid
Muscle
Liver
Hen
Egg
Amino Acid
mole %
Muscle
Liver
mole %
Alanine
Ala
9.2
9.5
9.9
Arginine
Arg
4.8
5.0
4.3
Aspartate +
Asparagine
Asx
6.6
7.9
Cysteine
Cys
2.4
1.5
Methionine
Met
2.6
2.2
2.3
Phenylalanine
Phe
3.3
3.6
4.1
7.9
Proline
Pro
4.8
5.7
5.7
1.8
Serine
Ser
10.6
6.3
6.7
Threonine
Thr
5.3
0.5
5.2
Tryptophan
Trp
1.0
0.7
1.0
Glutamate +
Glutamine
Glx
10.2
13.0
10.5
Glycine
Gly
5.9
8.7
8.0
Tyrosine
Tyr
3.0
2.2
2.6
Histidine
His
2.0
2.4
2.2
Valine
Val
8.0
6.1
6.8
Isoleucine
Ile
6.4
4.7
5.0
Leucine
Leu
8.4
8.0
9.1
Lysine
Lys
5.5
7.6
6.7
11/7/2015
Slide 10
Graphical Example of the Table Data as an Almost Too Busy Bargraph:
Amino Acid Composition of Select Protein Sources
Amino Acid Content
(mole fraction)
14%
Hen Egg
Muscle
Liver
12%
10%
8%
6%
4%
2%
0%
p
Tr
is
H
ys
C
et
M
r
Ty
e
Ph
rg
A
o
Pr
r
Th
s
Ly
ly
G
Ile
sx
A
l
Va
u
Le
la
A
lx
G
r
Se
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high
in
egg
each
are
double
low
in
egg
not very abundant
Slide 11
Example of an Annotated, Scanned Figure:
ESI-MS Spectrum of Horse Apomyoglobin
+14
Apomyoglobin
MW=16,951.2
+16
+18
+12
+20
+10
+22
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m/z
Slide 12
Conclusions
 You must have one or more Conclusions slides
 The Conclusions
 Reiterate the key points of what you have accomplished
 State (for the last time) what is important about what you have done
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Slide 13
Additional Constraints
At the time of your oral presentation, you need to:
1. Distribute a 1-page abstract of your talk to the class, TA's and
instructor
2. Distribute copies of the slides that you will use to your TA's &
instructor

Printed no more than 2 per page
3. Give an annotated appendix of all figures, tables, data, etc. to
your TA

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This appendix is the same as you would prepare for a regular
written laboratory.
Slide 14
Final Points

Plan on speaking for 30 (25-35) min with 10 min at the end for questions

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Practice your talk before you actually give it!
Be on time and be ready to go

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Technical problems will cost you valuable speaking time
You can use for your presentation
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Slides displayed from a computer projection device
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Angell B203 has a PC w/ USB port (not a Mac)
Overheads
Blackboard (as supplemental)
You get up to 20 points (your presentation is worth 50 points) for
participating in asking questions and discussing the presentations
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
If you not ask questions or contribute to the discussion of the talks, you don’t
get any points
Generally one question per talk for 4-5 talks will get you the 20 points
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Slide 15