24oC OD 18hrs A 1.1 1.0 0.9 OD (600nm) 0.8 0.7 0.6 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.5 0.4 0.3 0.2 0.1 0.0 Time (hours) 28oC OD 18hrs B 1.2 1.1 1.0 OD (600nm) 0.9 0.8 0.7 0.6 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.5 0.4 0.3 0.2 0.1 0.0 Time (hours) 37oC OD 18hrs C 0.9 0.8 OD (600nm) 0.7 0.6 0.5 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.4 0.3 0.2 0.1 0.0 Time (hours) Supplementary figure 2: Growth curves from the temporal.
Download ReportTranscript 24oC OD 18hrs A 1.1 1.0 0.9 OD (600nm) 0.8 0.7 0.6 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.5 0.4 0.3 0.2 0.1 0.0 Time (hours) 28oC OD 18hrs B 1.2 1.1 1.0 OD (600nm) 0.9 0.8 0.7 0.6 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.5 0.4 0.3 0.2 0.1 0.0 Time (hours) 37oC OD 18hrs C 0.9 0.8 OD (600nm) 0.7 0.6 0.5 1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3 0.4 0.3 0.2 0.1 0.0 Time (hours) Supplementary figure 2: Growth curves from the temporal.
24oC OD 18hrs
A
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
0
1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3
2 4 6 8 10 Time (hours) 28oC OD 18hrs 12 14 16 18
B
1.2
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
0
1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3
2 4 6 8 10 Time (hours) 37oC OD 18hrs 12 14 16 18
C
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
0
1668lux1 1668lux2 1668lux3 1572lux1 1572lux2 1572lux3
2 4 6 8 10 Time (hours) 12 14 16 18
Supplementary figure 2
: Growth curves from the temporal expression of Ifp and invasin assay. OD readings at 600 nm were taken at 30 minute intervals within the Anthos Lucy1 combined photometer and luminometer. Cultures were incubated at (A) 24°C (B) 28°C and (C) 37°C. Three biological replicates are shown for each strain, and each replicate was tested in triplicate.