The Green World’s Gift: Photosynthesis Objectives: 1. Explain why organisms need a supply of energy. 2.
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The Green World’s Gift: Photosynthesis Objectives: 1. Explain why organisms need a supply of energy. 2. Explain how energy is stored in/or released from ATP. 3. Explain the role of chlorophyll in photosynthesis. 4. Explain how the light dependent and the Calvin Cycle are related. 5. Identify the reactants and the products of photosynthesis. 6. Discuss the importance of photosynthesis to life on Earth!! Photosynthesis and Energy 1. Try to name something you eat that isn’t from a plant or from an animal that ate a plant. 2. All food comes from plants. Molecules of our bodies are made from food we eat, but plants make their own food from sunlight. Food made by plants and eaten by animals is used for: a) Creating macromolecules from monomers like glucose and amino acids. Making all the organic molecules found in living things! b) More importantly, food is used in cells generate cellular energy, ATP. Therefore: Plants are the nearly universal source of energy for all living things. ATP is the most important temporary energy storage molecule: 1. Biological work takes energy 2. Energy locked up in molecules is potential energy, however it is not available for work. 3. The potential energy from food breakdown is used to drive the synthesis of ATP (like recharging a battery). 4. This high energy ATP has stored energy that can be used by cells to do a variety of actions. 5. ATP energy can be released at any time by breaking off the third phosphate Plants take energy-poor reactants (water and carbon dioxide) and use solar energy to drive the uphill reaction of trapping those reactants in complex, ordered bonds of glucose. Photosynthesis • The goal of photosynthesis :_________________ ________________________________________ • Photosynthesis is necessary because glucose is needed for ___________________. • The energy required to synthesize (make) glucose comes from ___________________________. which does not have mass (weight); • The materials needed to synthesize glucose come from ______and _______. Photosynthesis Equation: •6CO2 + 6H2O + light energy C6H12O6 + 6O2 CO2 is from ________ H2O is from ________ A. Nature of Light 1. Energetic rays have different wavelengths in a spectrum from gamma rays to radio waves, only a portion of which is visible light: PLANT PIGMENTS: • When we see a green plant: ______________- light is absorbed while _______________light is reflected. 3. Why is a black car is hotter on a sunny day than a white car? 4. Chlorophyll is a pigment molecule in plants (in chloroplasts), that absorbs light. What color(s) do you think chlorophyll absorbs?? 5. Plants have other pigments too– each with their own specific wavelength of absorption. The other pigments increase the wavelengths, (colors), of light that the leaf can use for photosynthesis! Plant Pigments • Chlorophyll is a pigment molecule in plants (in chloroplasts), that absorbs light • Plants have other pigments too– each with their own specific wavelength of absorption. The other pigments increase the wavelengths, (colors), of light that the leaf can use for photosynthesis! Photosynthesis is driven by only part of the visible spectrum (blue and red); plant pigments in the green plant reflects green and absorbs blue and red. Note: The higher the peak, the stronger the absorption of that wavelength of light. Tour of a leaf, where plants absorb light: Blade Leaf section, epidermis, stomata, mesophyll Chloroplasts, inner and outer membranes, Grana and stroma Thylakoid membrane, and compartment Stomata… are pores in the leaf surface that allow gases in or out. What gases pass in and out of stomata? (Singular = stomate) Leaf cross section Photosynthesis mostly occurs in the mesophyll cells. A. Photosynthesis occurs in two essential phases. 1. Light-dependent Reactions =“photo” of photosynthesis. a) Power of sunlight excites electrons in pigment molecules. b) Excited electrons are carried down transport chain to provide energy for the second phase . c) Pigment electrons are replaced by electrons stripped from water, making O2 gas. ((This is where the water is used and the oxygen is produced!)) d) Photosystems are the working units that absorb solar energy. 1. Clusters of hundreds of pigment molecules serve as antenna to absorb solar energy. 2. Reaction center of aggregate contains pair of chlorophyll molecules with electrons that absorb the energy and jump to electron carrier molecules: e) Energy transfer is possible using redox reactions. 1. One substance loses electrons (oxidized) while another gains electrons (reduced). = ‘Redox’ 2. Electrons move down the energy hill, losing energy as they go (Analogy: the passing of a hot potato warming each hand as it drops, giving off some heat as it goes. The last person to get the potato gets some heat and food as well.) …until the final recipient of the electrons, in this case is + NADP which becomes NADPH + Follow the pathway of the Light Reactions: 1. Photosystem II absorbs solar energy. 2. Electron jumps to the primary electron acceptor. 3. Chlorophyll is left without an electron, but it is replaced by the splitting of water into H+ ions, electrons and O2. 4. Ejected electron falls back down the energy hill through a series of electron transfer molecules called an ‘electron transport chain, until it reaches Photosystem I (another reaction center also receiving solar energy that excites its electrons). **In the process some ATP is made** 5. Again, energized electrons from Photosystem I are transferred back down another energy hill (electron transport chain), until they are received by NADP+, an electron carrier that ferries electrons to the second stage, the light-independent stage of photosynthesis. B. Importance of the light-dependent phase 1. Oxygen formation O2 2. Energized electrons being transferred, (not just giving off heat or fluorescing), and ferried by NADPH for stage 2. NADPH 3. Formation of ATP, which is also used to power the second stage, the light-independent reactions, stage 2. ATP ATP NADPH O2 B. Light-independent Reactions-Synthesis Phase = The Calvin Cycle a) ATP and NADPH are not good permanent storage molecules but are good carriers. b) Plants covert the energy in ATP and NADPH into the bonds in a glucose molecule. Electrons from carriers are brought together with CO2 and H2O to make this glucose. Simplified: 1. Carbon Fixation = the process of taking CO2 from the atmosphere and creating an organic compound from it. 2. Enzyme called rubisco is responsible for this process: it brings together CO2 and a five carbon sugar. More Detail: Importance of Light Independent Reactions: **Carbon is fixed into organic compounds!! The glucose can be converted into the other organic substances in the plant AND it supplies animals with food. A. Glitch in the system—Photorespiration 1. Rusbisco often combines O2 instead of CO2 with RuBP, unproductively. 2. Occurs one O2 for every three CO2. 3. Undercuts food production in crops that use C3 cycle. 4. Especially problematic in hot weather because of evaporation of water. Plant closes stomata in leaves to prevent evaporation, but as water is kept in, CO2 is kept out. As the light-dependent reactions continue, O2 builds up, combining with RuBP unproductively. 1. Grasses, corn, sugarcane, and sorghum 2. Use a different enzyme located in bundle-sheath cells: Figure 8.10 3. Costs ATP to shuttle CO2 to bundle-sheath cells; in sunny climates this is not an issue, because with abundant sunlight, ATP is plentiful. 4. In northern climates, C4 plants are not as well adapted. Video C-4 Pathway • file:///D:/bc_campbell_biology_7/0,7052,31 17218-,00.html CAM Plants—another adaptation that saves water in hot climates (Section 8.8) A. Cactus, pineapple, mint, and orchid B. Close stomata during the day, open at night C. Start C4 metabolism at night by fixing CO2 but wait for day to use abundant ATP to finish. D. Comparison of three strategies: Figure 8.12 Summary of Photosynthesis in most kinds of plants Review The Process in a leaf: Review the Process in a Chloroplast Some autotrophic bacteria can survive without light Instead they use the energy in inorganic compounds like Hydrogen sulfide or ammonia to make organic compounds Such organisms are called Chemosynthetic autotrophs Today these organisms are found in extreme environments like deep sea vents and sulfur springs How do organisms use the food made by photosynthesis??. 3. Oxygen needed for respiration is produced as a byproduct of photosynthesis. The End