Transcript Development of Amylomaize Hybrids using GEM Germplasm Mark Campbell, Andrew Green, Joseph Bell (Truman State University) Jay-lin Jane, Hongxin Jiang (Iowa State University) Yusheng WU (South Dakota State University) Chicago ASTA.

Development of
Amylomaize
Hybrids using
GEM Germplasm
Mark Campbell, Andrew Green,
Joseph Bell (Truman State
University)
Jay-lin Jane, Hongxin Jiang
(Iowa State University)
Yusheng WU (South Dakota
State University)
Chicago ASTA meeting–
December 5th 2007
Applications of high-amylose starch…..
– Gum candies
– Adhesives
– Biodegradable plastics
– Food coatings
Resistant Starcha nutraceutical
• Cold potatoes
• Green bananas
• Novel industrial
synthetic sources
• High amylose corn
Normal
starch
Resistant
starch
Resistant Starcha nutraceutical
• Cold potatoes
• Green bananas
• Novel industrial
synthetic sources
• High amylose corn
Normal
starch
Resistant
starch
Resistant starch feeds
bifidobacterium in large intestine
Butyrate is good for us!!!!!
-decreases colon cancer cell formation
-decreases cholesterol
-lowers blood pressure
-weight control
ae – amylose extender
How to Develop Amylomaize
VII Class Hybrids from
Scratch:
First step –
Find
‘modifier
genes’
Plant
introductions
GEM breeding
crosses
Visually selected
ae types kernels
Screened many ae-type F2 in the lab
GUAT209:S13//OH43ae/H99ae
GEMS-0067
is now freely
available!!
Next……Hybrid Development
From GEMS-0067
both SS and NSS
parents must be made
SS inbreds (S1)
NSS S1 inbreds (S1)
SS inbreds
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
UR11003:S0302-937-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
(CHIS740:S1411a-783-2-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
(CUBA164:S1511b-325-1-B///GUAT209:S13//OH43ae/H99ae 1-2-1 ) - 1
(UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-2-2) - 1
(CUBA164:S2012-966-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(AR16026:S1704-153-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(2152-03_DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
NSS inbreds
DKXL370:N11a20-234-2-B-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
DXKL370:N11a20-322-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
DKXL370:N11a20-199-2-B-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
CH05015:N1502-86-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
AR03056:N09-191-1-B-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
DK212T:N11a12-191-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
BARBGP2:N08a18-332-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
FS8B(T):N11a-87-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
CH05015:N1204-57-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
UR13085:N0215-14-1-B-B-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
DREP150:N2011d-624-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
FS8B(T):N11a-110-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
(DKXL370:N11a20-199-2-B-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
FS8B(T):N11a-322-1-B-B////DKXL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
(CH05015:N15-3-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(DKXL370:N11a20-36-2-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(UR13085:N0215-14-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
(CH05015:N12-183-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
(SCR01:N1310-358-1-B-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
• Yield trial – 2007
– 83 GEM (SS) x GEM(NSS)
entries
– 2 proprietary hybrids
obtained from a company
whose name must not be
spoken
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
10
(AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
42
(DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
50
(AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
15
(DXKL370:N11a20-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
3
(DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
23
(CH05015:N1502-86-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
21
(BARBGP2:N08a18-332-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
43
(DXKL370:N11a20-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
8
(CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
61
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
11
(DK212T:N11a12-191-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
51
(FS8B(T):N11a-87-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
40
(AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
52
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
1
(AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
80
((2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((CH05015:N15-3-1-B-B///GM67//O/Hae 1-2-1) - 1)
39
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-937-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
25
(AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
12
(AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
20
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
77
((2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((SCR01:N1310-358-1-B-B///GM67//O/Hae 6-2-1-1) - 1)
9
(CH05015:N1502-86-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
62
(AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
46
(CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
29
(FS8B(T):N11a-87-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
27
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
28
(DXKL370:N11a20-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
57
(CH05015:N1502-86-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
6
(FS8B(T):N11a-110-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
24
(DK212T:N11a12-191-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
47
(CH05015:N1502-86-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
Yield
TWTa
14
Average Performance over MO and IA locations
MOIST
Entry
Pedigree
(bu/ac)
111.8
111.7
108.6
106.7
106.5
106.3
103.5
102.3
101.9
101.8
101.2
100.2
100.1
99.6
99.5
99.0
98.3
98.2
98.1
97.6
96.7
96.3
95.6
95.3
94.6
94.0
93.5
93.4
92.8
92.4
92.4
91.4
27.2
25.9
28.0
27.1
25.3
27.3
26.4
24.9
26.8
25.7
26.9
29.2
27.3
28.6
26.3
28.8
25.6
28.8
26.3
27.8
28.0
25.3
25.0
27.7
25.0
28.3
29.3
26.8
26.4
26.6
28.6
29.0
52.4
47.9
49.9
49.4
50.9
48.0
49.9
49.7
47.0
48.0
52.2
46.5
48.1
50.4
50.8
48.9
49.6
48.9
49.2
48.1
48.4
50.9
48.8
49.4
50.8
49.2
50.7
51.5
47.4
48.3
47.2
48.5
59
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
60
(AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
63
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
48
(AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
58
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
70
(DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
45
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
49
(DK212T:N11a12-191-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
53
(AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
16
(FS8B(T):N11a-87-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
37
(DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
36
(CH05015:N1502-86-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
41
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
72
Check 1
84
((CUBA164:S2012-966-1-B///GM67//O/Hae1-2-1) - 1 )x((CH05015:N15-3-1-B-B///GM67//O/Hae 6-2-1-1) - 1)
2
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
67
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
30
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
34
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
68
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
65
(BARBGP2:N08a18-332-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
35
(AR03056:N09-24-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
78
((CHIS740:S1411a-783-2-B-B /// GM67//O/Hae 1-2-1 )x((DKXL370:N11a20-199-2-B-B-B /// GM67//O/Hae 1-2-1)
22
(CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
19
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
71
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
73
((CUBA164:S1511b-325-1-B///GM67//O/Hae 1-2-1 ) - 1 )x((CH05015:N15-3-1-B-B///GM67//O/Hae 6-2-2-1) - 1)
64
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
85
Check 2
32
(CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
69
(FS8B(T):N11a-110-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
26
(DREP150:N2011d-624-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
54
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
4
(DXKL370:N11a20-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
18
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
44
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
17
(FS8B(T):N11a-110-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GM67//O/Hae)
91.3
91.2
91.2
89.6
89.3
89.3
89.1
88.7
88.4
88.0
87.9
87.7
87.4
87.1
86.9
86.8
86.2
86.1
85.4
85.4
85.3
85.2
84.9
84.3
84.3
84.3
83.8
83.5
83.5
83.3
82.9
82.8
82.7
82.5
82.4
82.2
80.6
27.3
27.7
27.5
26.9
27.0
27.6
29.1
27.9
28.2
26.8
28.8
27.6
24.1
25.0
24.7
26.5
24.9
27.9
28.6
25.8
26.2
28.1
27.3
28.2
28.9
27.5
24.3
25.4
25.6
27.7
23.7
28.3
26.5
25.7
27.2
26.7
25.2
49.8
47.7
50.1
48.6
46.6
49.7
47.2
47.6
49.0
48.0
48.3
48.9
51.0
49.2
49.8
47.2
48.8
52.8
50.4
51.0
50.7
48.2
51.5
47.2
49.8
48.4
49.6
49.3
49.9
50.7
50.0
51.2
50.4
48.8
48.3
49.2
47.6
80.5
80.1
79.7
78.2
78.0
77.9
77.9
77.5
76.9
75.4
74.3
74.3
71.2
70.0
68.9
52.9
25.2
26.0
47.8
48.7
48.9
49.6
48.2
49.5
47.1
47.2
48.1
48.1
50.0
46.9
47.5
45.7
48.7
48.2
mean of entries across location and replication
88.9
26.8
49.0
LSD
27.8
3.57
3.3
83
((2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GM67//O/Hae1-2-1) - 1 )x((DKXL370:N11a20-36-2-B///GM67//O/Hae7-2-2-1) - 1)
31
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
82
((FS8A(S):S09-362-1-B///GM67//O/Hae 2-2-2) - 1 )x((UR13085:N0215-14-1-B///GM67//O/Hae7-2-2-1) - 1)
56
(BARBGP2:N08a18-332-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
81
((CH05015:N12-183-1-B-B///GM67//O/Hae 6-2-1-1) - 1 )x((CUBA164:S2012-966-1-B///GM67//O/Hae 6-2-1-1) - 1)
38
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-937-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
79
((CH05015:N12-183-1-B-B///GM67//O/Hae 6-2-1-1) - 1 )x((UR10001:S1813-257-1///GM67//O/Hae 6-2-1-1) - 1)
74
((AR16026:S1704-153-1-B///GM67//O/Hae 6-2-1-1) - 1 )x((CH05015:N15-3-1-B-B///GM67//O/Hae 6-2-1-1) - 1)
55
(UR13085:N0215-14-1-B-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
13
(AR03056:N09-191-1-B-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
66
(CH05015:N1204-57-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
33
(DK212T:N11a12-191-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
5
(FS8B(T):N11a-87-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
7
(FS8B(T):N11a-322-1-B-B////DKXL370:N11a20-31-1-B-B-SIB///GM67//O/Hae )x(CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GM67//O/Hae)
76
((2152-03_DK888_S11_F2S4_9205-Blk24/00-sib///GM67//O/Hae7-2-2-1) - 1 )x((CH05015:N15-3-1-B-B///GM67//O/Hae 6-2-1-1) - 1)
75
((CUBA164:S2012-966-1-B///GM67//O/Hae7-2-2-1) - 1 )x((SCR01:N1310-358-1-B-B///GM67//O/Hae 6-2-1-1) - 1)
Yield
(ave.)
Yield
(Max)
Moist
(ave)
Missouri
68.3
95.4
26.2
Iowa
109.5
148.9
27.3
28.3
26.2
26.9
25.1
25.2
26.1
24.7
27.8
26.3
24.6
28.1
25.4
26.2
26.0
Five entries were found on IA and MO list of top 15
entries by yield.
IA
MO
14
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-BB-SIB///GEMS0067//O/H ae x CUBA164:S2012-444-1-B-Bsib////AR16035:S02-615-1-B-B///GEMS0067//O/H ae)
138.7
85.0
42
(DKXL370:N11a20-234-2-B-B-B////DKXL370:N11a20-31-1-BB-SIB///GEMS0067//O/H ae x CHIS775:S1911b-120-1-B-BB////AR16035:S02-615-1-B-B///GEMS0067//O/H ae)
137.6
79.6
50
(AR03056:N09-250-1-B-B-B////DKXL370:N11a20-31-1-B-BSIB///GEMS0067//O/H ae x CHIS775:S1911b-120-1-B-BB////AR16035:S02-615-1-B-B///GEMS0067//O/H ae)
134.8
78.6
10
(AR03056:N09-182-1-B-B-B////DKXL370:N11a20-31-1-B-BSIB///GEMS0067//O/H ae x CUBA164:S1511b-325-1-BB////AR16035:S02-615-1-B-B///GEMS0067//O/H ae)
123.8
95.4
61
(DKXL370:N11a20-199-2-B-B-B////DKXL370:N11a20-31-1-BB-SIB///GEMS0067//O/H ae x DKB844:S1601-73-1-B-BB////AR16035:S02-615-1-B-B///GEMS0067//O/H ae)
125.2
77.3
Averaged performance of SS lines by yield
SS code
SS inbred
n
Yield
(bu/ac)
MOIST
TWT
10
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
94.5
27.2
49.0
12
CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
13
93.4
27.0
49.2
2
91.7
25.3
49.4
1
(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1
11
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
13
91.6
26.6
47.8
14
DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
90.1
27.1
49.0
16
UR11003:S0302-937-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
2
88.1
27.0
49.2
13
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
86.5
28.1
49.6
15
UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
10
85.8
26.1
49.7
4
(CHIS740:S1411a-783-2-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
1
84.9
27.3
51.5
5
(CUBA164:S1511b-325-1-B///GUAT209:S13//OH43ae/H99ae 1-2-1 ) - 1
1
83.8
24.3
49.6
9
(UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
2
80.7
25.9
48.5
8
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-2-2) - 1
3
80.4
26.0
48.6
7
(CUBA164:S2012-966-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
2
80.4
26.0
48.6
6
(CUBA164:S2012-966-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
1
78.0
25.1
48.2
3
(AR16026:S1704-153-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
1
77.5
24.7
47.2
2
(2152-03_DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae7-2-2-1) -
1
68.9
26.0
48.7
Averaged performance of SS lines by yield
SS code
SS inbred
n
Yield
(bu/ac)
MOIST
TWT
10
CHIS775:S1911b-120-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
94.5
27.2
49.0
12
CUBA164:S2012-444-1-B-B-sib////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
13
93.4
27.0
49.2
2
91.7
25.3
49.4
1
(2011-01_SE32_S17_F2S4_9148-Blk22/00-sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1
11
CUBA164:S1511b-325-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
13
91.6
26.6
47.8
14
DKB844:S1601-73-1-B-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
90.1
27.1
49.0
16
UR11003:S0302-937-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
2
88.1
27.0
49.2
13
CUBA164:S2012-966-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
11
86.5
28.1
49.6
15
UR11003:S0302-1011-1-B-B////AR16035:S02-615-1-B-B///GUAT209:S13//OH43ae/H99ae
10
85.8
26.1
49.7
4
(CHIS740:S1411a-783-2-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
1
84.9
27.3
51.5
5
(CUBA164:S1511b-325-1-B///GUAT209:S13//OH43ae/H99ae 1-2-1 ) - 1
1
83.8
24.3
49.6
9
(UR10001:S1813-257-1///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
2
80.7
25.9
48.5
8
(FS8A(S):S09-362-1-B///GUAT209:S13//OH43ae/H99ae 2-2-2) - 1
3
80.4
26.0
48.6
7
(CUBA164:S2012-966-1-B///GUAT209:S13//OH43ae/H99ae7-2-2-1) - 1
2
80.4
26.0
48.6
6
(CUBA164:S2012-966-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
1
78.0
25.1
48.2
3
(AR16026:S1704-153-1-B///GUAT209:S13//OH43ae/H99ae 6-2-1-1) - 1
1
77.5
24.7
47.2
2
(2152-03_DK888_S11_F2S4_9205-Blk24/00-sib///GUAT209:S13//OH43ae/H99ae7-2-2-1) -
1
68.9
26.0
48.7
TWT
MOIST
n
Yield
(bu/ac)
SS code
SS inbred
CHIS775:S1911b-120-1-B-BB////AR16035:S02-615-1-B10 B///GUAT209:S13//OH43ae/H99ae
11
94.5
27.2
49.0
CUBA164:S2012-444-1-B-Bsib////AR16035:S02-615-1-B12 B///GUAT209:S13//OH43ae/H99ae
13
93.4
27.0
49.2
2
91.7
25.3
49.4
13
91.6
26.6
47.8
1
(2011-01_SE32_S17_F2S4_9148-Blk22/00sib///GUAT209:S13//OH43ae/H99ae1-2-1) - 1
CUBA164:S1511b-325-1-BB////AR16035:S02-615-1-B11 B///GUAT209:S13//OH43ae/H99ae
Averaged performance of SS lines by yield
NS code
NS line
n
Yield
(bu/ac)
MOIST
TWT
29
DKXL370:N11a20-234-2-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
2
107.4
27.7
48.9
31
DXKL370:N11a20-322-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
96.1
26.2
49.5
28
DKXL370:N11a20-199-2-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
6
94.4
26.6
50.7
26
CH05015:N1502-86-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
5
94.2
26.9
48.7
23
AR03056:N09-191-1-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
17
92.6
27.3
48.7
27
DK212T:N11a12-191-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
88.9
28.4
47.0
24
BARBGP2:N08a18-332-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
88.6
26.0
50.0
34
FS8B(T):N11a-87-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
88.3
26.9
48.2
25
CH05015:N1204-57-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
5
87.7
26.2
49.3
35
UR13085:N0215-14-1-B-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
6
87.4
27.5
48.5
30
DREP150:N2011d-624-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
86.7
28.2
49.7
32
FS8B(T):N11a-110-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
85.3
25.2
48.6
19
(DKXL370:N11a20-199-2-B-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
1
84.9
27.3
51.5
33
FS8B(T):N11a-322-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
9
84.0
26.9
49.6
Averaged performance of SS lines by yield
NS code
NS line
n
Yield
(bu/ac)
MOIST
TWT
29
DKXL370:N11a20-234-2-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
2
107.4
27.7
48.9
31
DXKL370:N11a20-322-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
96.1
26.2
49.5
28
DKXL370:N11a20-199-2-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
6
94.4
26.6
50.7
26
CH05015:N1502-86-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
5
94.2
26.9
48.7
23
AR03056:N09-191-1-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
17
92.6
27.3
48.7
27
DK212T:N11a12-191-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
88.9
28.4
47.0
24
BARBGP2:N08a18-332-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
88.6
26.0
50.0
34
FS8B(T):N11a-87-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
4
88.3
26.9
48.2
25
CH05015:N1204-57-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
5
87.7
26.2
49.3
35
UR13085:N0215-14-1-B-B-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
6
87.4
27.5
48.5
30
DREP150:N2011d-624-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
86.7
28.2
49.7
32
FS8B(T):N11a-110-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
3
85.3
25.2
48.6
19
(DKXL370:N11a20-199-2-B-B-B /// GUAT209:S13//OH43ae/H99ae 1-2-1
1
84.9
27.3
51.5
33
FS8B(T):N11a-322-1-B-B////DKSL370:N11A20-32-2-1-B-B///GUAT209:S13//OH43ae/H99ae
9
84.0
26.9
49.6
n
Yield
(bu/ac)
MOIST
29
DKXL370:N11a20-234-2-B-BB////DKXL370:N11A20-32-2-1-BB///GUAT209:S13//OH43ae/H99ae
2
107.4
27.7
31
DXKL370:N11a20-322-1-BB////DKXL370:N11A20-32-2-1-BB///GUAT209:S13//OH43ae/H99ae
4
96.1
26.2
28
DKXL370:N11a20-199-2-B-BB////DKXL370:N11A20-32-2-1-BB///GUAT209:S13//OH43ae/H99ae
6
94.4
26.6
26
CH05015:N1502-86-1-BB////DKXL370:N11A20-32-2-1-BB///GUAT209:S13//OH43ae/H99ae
5
94.2
26.9
NS
code
NS - lines
Studies on the inheritance and
gene action of high amylose
modifier genes
High amylose modifiers
often treated multiple
genes that influence a
quantitative trait.
V. Fergason, 1994
In: Specialty Corns’
Generation Means Analysis
• A triploid model
proposed by Bogyo et
al (1988) is being
employed
• GEMS-0067, H99ae
and the F1 were
selfed and intermated
to produce 9 distinct
generations.
70
South
Dakota
60
50
Missouri
H
H/F1
H/G
F1/H
F1
F1/G
G/H
G/F1
40
G
% amylose
80
generation
Source
DF
MS
F
Population
9
1014.10
49.14**
Location
1
448.90
21.61**
Year
1
131.10
6.35**
Pop × Loc
9
49.10
2.38**
Pop × Year
4
59.80
2.90**
Loc × Year
1
2.24
0.11
Pop × Loc × Year
4
30.60
1.48
Error
1240
20.63
**: significant at 1% probability level.
Amylose
based on
colorimetric
method
GEMS-0067
amylose
amylopectin
H99 ae
Amylose based on
gel permeation
chromatography
at ISU
Normal
starch
Corn with
ae allele
30
40
50
60
70
(oC)
80
90
100
QTL analysis of high
amylose modifier
genes
High amylose
modifier?
amylose-extender (ae) locus
sbe1a-Mu (W64A-BC4); ae self
sbe2a-Mu (W64A-BC4) self
ae
Obtained from Mark
Guiltinan, Penn State
using the Trait Utility
System for Corn (TUSC)
developed by Pioneer HiBred International, Inc.
• Distribution of
amylose values
among F2 ears
in the GEMS0067 x H99ae
mapping
populations
• Appears to be
more than one
gene involved
Question?