HLA TYPING JS Dhaliwal INSTITUTE FOR MEDICAL RESEARCH WHAT IS HLA NOMENCLATURE TYPING TYPING STRATEGY.
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HLA TYPING JS Dhaliwal INSTITUTE FOR MEDICAL RESEARCH WHAT IS HLA NOMENCLATURE TYPING TYPING STRATEGY Major Histocompatability Complex Class I: 43 KDa b2 microglobulin 12 kDA Class II a chain 30-34 kDA b chain 26-29 kDA Class II Antigen NOMENCLATURE Nomenclature HLA-A*0201 A – gene locus 02 – specificity, serological 01 – allele number Nomenclature DRB1*040501 DRB1 – gene locus 04 – specificity, serological 05 – allele number 01 – Silent mutation Nomenclature HLA-DRB1*13010102 an allele which contains a mutation outside the coding region Nomenclature HLA-DRB1*13010102 Nomenclature HLA-B*2705/10 HLA-B*2705 or HLA-B*2710 Nomenclature HLA-B*27EG Nomenclature Nomenclature Nomenclature Nomenclature HLA-A*9201 HLA-B*9501 Nomenclature Nomenclature Nomenclature Nomenclature HLA-B*2705/10 HLA-B*2705 or HLA-B*2710 Common and Well documented TYPING TYPING METHODS When is high resolution typing required TYPING METHODS • Sequence Specific Primer • Sequence Specific Oligonucleotide • Sequencing PCR HLA B*2704 HLA B*2706 HLA B*2707 SSO SBT Typing strategy, other considerations • • • • • Class II first Class I Repeat Class I and Class II Names Family tree A24 B48 DR15 DQ6 A2 B51 DR14 DQ5 A11 B13 DR14 DQ5 A24 B48 DR15 DQ6 A11 B13 DR15 DQ6 A24 B48 DR15 DQ6 A11 B13 DR15 DQ6 A2 B51 DR14 DQ5 A11 B13 DR14 DQ5 A11 B13 DR15 DQ6 A11 B13 DR15 DQ6 A11 B13 DR15 DQ6 Other activities • Malaysian Marrow Donor Registry • Testing for Solid Organ Transplants – Cytotoxic Cross match – Panel Reactive Antibodies, Cl I & II – Flow Cross match – Identification of Ig type Contact • [email protected] • [email protected] • [email protected]