Bacterial Transformation

A Towson University MDLL Lab

Meet Alba the GFP Bunny, and...

...the Spider Goat!

What do you think of animals that have been genetically engineered to possess genes from other organisms?

Transgenic Organisms

Foreign DNA added to an organism + + NSF video explores spider goat uses and how scientists developed them.

Bacteria as Transgenic Organisms

Benefits to Bacteria

• Bacteria are inexpensive to grow.

• Bacteria have simpler genome.

• Some bacteria “transform” easily.

• Universal Code means bacteria read eukaryotic DNA.

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How Does Transformation Work?

First, explore the concepts of – Culturing bacteria & aseptic techniques – Plasmids, vectors, and marker genes – Bacterial antibiotic resistance – Bacterial competence, transformation – Restriction enzymes and PCR Next, read your scenario.

Then explain the basics to your lab assistant, Brad!

Develop Your Lab Plan 1. What research question are you trying to answer?

2. What will go on each of your four plates and why?

3. What plates will serve as your controls? Why?

Lab Overview

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Click on the image to go to a PBS video overview of bacterial transformation.

Pre-Lab Technique Practice • Streak Plate Challenge – Remember your aseptic technique!

• Micropipette Challenge Golden Rules of Pipetting

1. Don’t rotate the volume adjuster beyond the upper or lower range of the pipette; this can damage it.

2. Don’t use a pipette without a tip on it. If this happens, liquid gets into the opening of the pipette and can damage the mechanism inside.

3. Don’t lay down the pipette that has a tip filled with liquid. If this happens, liquid can get inside the pipette and can damage it. 4. Use new pipette tips between samples to prevent

contamination. www.sks-science.com

Your Turn in the Lab!

• Carefully follow the protocol.

• Temperatures and times for each step are critically important!

• Wash hands and work bench thoroughly before and after lab work.

• Ask questions if you are confused.

• Clean up following your instructor’s directions.

Did your cells transform?

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= How do you know?