Transcript Evaluation of Advion™, a New Indoxacarb-based Bait Developed for Control of the Red Imported Fire Ant (Solenopsis invicta Buren) LT Barry Furman, MSC, USN Navy.

Evaluation of Advion™, a New
Indoxacarb-based Bait Developed
for Control of the Red Imported
Fire Ant (Solenopsis invicta Buren)
LT Barry Furman, MSC, USN
Navy Entomology Center of
Excellence
Experimentation
• Broadcast treatments
1. Effectiveness at controlling S. invicta, Advion™ vs.
Amdro®
2. Effectiveness at controlling multiple ant species
Military Implications
• Infest >316 million acres, 14 states
- Florida west to California
- Texas north to North Carolina
• Existing/potential problem for DoD
installations across much of U.S.
Life Stages in a Mature Fire Ant Colony
Fertile
Queen
Queen
Larvae
Pupae
Workers (all sterile females)
minor major
Egg
Egg
Clutch
(10+Clutch
eggs)
Worker Life Stages
Reproductive Life Stages
Larvae
Female (future queen)
Pupae
Male
Indoxacarb
• Indoxacarb
- discovered in 1991 (E.I. DuPont de Nemours & Co.)
- insect must metabolize
toxic metabolite (JT333)= sodium channel blocker
● severe neurotoxic symptoms (paralysis), death
●
Indoxacarb
• RIFA control: bait
- Advion™
0.045% indoxacarb
● EPA approval: August 2004
●
Experiment 1
• Single field experiment
- June 4-July 23, 2004
• Private game ranch
- between Corpus Christi/
San Antonio, Texas
- ~12,000 acres
dove, quail, deer hunting
● cattle grazing
●
Experiment 1
• Treatments
- 0.045% Advion™ (1.5 lbs/acre)
- 0.73% Amdro® (1.5 lbs/acre)
- 0.0% Advion™ (1.7 kg/ha)
- control: untreated
• 3 replicates/treatment
- replicate = 1/3 acre plot w/>10 RIFA mounds
- >15 m between plots
• Mounds: flagged, activity graded
Experiment 1
• Day 0 (7:00-9:00 a.m.)
- treatments applied
• Days 1-7 (7:00-9:00 a.m.)
- readings once daily
• Days 21,49 (7:00-9:00 a.m.)
- final daily readings
Experiment 1 Results
• Mean cumulative daily colony mortality
- after 7 days
Advion™: 96%
● Amdro®: 43%
●
- after 21 days
● Advion™significantly
● Advion™:
99%
● Amdro®: 86%
- after 49 days
●
no change
greater
Treat 1- 0.0% Advion
100
Treat 2- 0.73% Amdro
90
Treat 3- 0.0% Advion followed by
0.045% Advion
Treat 4- 0.045% Advion
Mean Percent Colony Mortality +/- SD
80
70
60
50
40
30
20
10
0
0
1
2
3
4
5
6
7
21
49
Time (days)
Figure 1. A comparison of the mean percent RIFA colony mortality resulting from label-rate broadcast treatment with
0.045% Advion™, 0.73% Amdro®, and 0.0% Advion™ (contained no indoxacarb), and from pre-baiting broadcast treatment
consisting of treatment with 0.0% Advion™ (contained no indoxacarb) followed 1 h later by treatment with 0.045% Advion™.
Experiment 2
• Single field experiment
- June 4-July 23, 2004
• Private game ranch
• 1 treatment
- 0.045% Advion™ (1.5 lbs/acre)
- control: left untreated
• 10 replicates
- replicate= 1/10 acre plot
- >15 m between plots
Experiment 2
• Survey protocol
- 10 baited 8 ml vials per plot
●
5 w/tuna
● 5 w/60% sugar water
- dispersal
placed out @ 9:00 a.m.
● 2-3 m intervals
● picked up @ 10:00 a.m.
●
- species richness, abundance
Experiment 2
• Pre-treat
- survey
• Day 0
- treatment applied
• Days 1-7
- survey once daily
• Days 21,49
- final daily survey
Experiment 2 Results
• 4 species prevalent
-
S. invicta (RIFA)
M. pharaonis (pharaoh ant)
P. barbatus (harvester ant)
D. pyramicus (pyramid ant)
180
160
Mean Number of Ants +/- SD
140
120
S. invicta- control plots
S. invicta- treatment plots
100
80
60
40
20
0
0
1
2
3
4
5
6
7
21
49
Time (days)
Figure 2. Comparison of the mean number of Solenopsis invicta collected per plot prior to treatment, and in subsequent
days following treatment. The treated plots were treated with a label-rate broadcast treatment of 0.045% Advion™ and the
control plots were left untreated.
160
M. pharaonis- control plots
M. pharaonis- treatment plots
140
Mean Number of Ants +/- SD
120
100
80
60
40
20
0
0
1
2
3
4
5
6
7
21
49
Time (days)
Figure 3. Comparison of the mean number of Monomorium pharaonis collected per plot prior to treatment, and in
subsequent days following treatment. The treated plots were treated with a label-rate broadcast treatment of 0.045% Advion™
and the control plots were left untreated.
25
P. barbatus- control plots
P. barbatus- treatment plots
Mean Number of Ants +/- SD
20
15
10
5
0
0
1
2
3
4
5
6
7
21
49
Time (days)
Figure 4. Comparison of the mean number of Pogonomyrmex barbatus collected per plot prior to treatment, and in
subsequent days following treatment. The treated plots were treated with a label-rate broadcast treatment of
0.045% Advion™ and the control plots were left untreated.
60
D. pyramicus- control plots
D. pyramicus- treatment plots
Mean Number of Ants +/- SD
50
40
30
20
10
0
0
1
2
3
4
5
6
7
21
49
Time (days)
Figure 5. Comparison of the mean number of Dorymyrmex pyramicus collected per plot prior to treatment, and in
subsequent days following treatment. The treated plots were treated with a label-rate broadcast treatment of
0.045% Advion™ and the control plots were left untreated.
Experiment 2 Results
• Effectiveness of treatment
- after 1 d: 92-100% decrease, all species
- control of S. invicta and P. barbatus: at least 7 wk
- control of M. pharaonis and D. pyramicus: 3-7 wk
Overall Conclusions
• Advion™: fastest acting fire ant bait
available
• Extremely effective at controlling fire ants
• Effective at controlling multiple ant species
QUESTIONS??