Transcript Eau d’coli N Kuldell for 20.020 Spring 2011 System level description: initial planning Input e.g. precursor Black box functions WGD and BGD Output e.g. smell WGD: Wintergreen Generating Device BGD: Banana Generating Device.

Eau d’coli
N Kuldell for 20.020
Spring 2011
System level description: initial planning
Input
e.g.
precursor
Black box functions
WGD
and
BGD
Output
e.g.
smell
WGD: Wintergreen Generating Device
BGD: Banana Generating Device
System level description: scent production
Input
e.g.
precursor
WGD
Black box functions
BGD
Output
e.g.
smell
WGD: Wintergreen Generating Device
BGD: Banana Generating Device
System level description: scent production
Input
e.g.
precursor
WGD
Black box functions
BGD
Output
e.g.
smell
WGD: Wintergreen Generating Device
BGD: Banana Generating Device
System level description: precursor production
Input
e.g. cell
growth
WGD
Black box functions
BGD
Output
e.g.
smell
saGD
and
iaGD
WGD: Wintergreen Generating Device
BGD: Banana Generating Device
saGD: salicylic acid Gen Dev
iaGD: isoamyl alcohol Gen Dev
System level description: precursor production
Input
e.g. cell
growth
saGD
iaGD
WGD
Black box functions
BGD
Output
e.g.
smell
WGD: Wintergreen Generating Device
BGD: Banana Generating Device
saGD: salicylic acid Gen Dev
iaGD: isoamyl alcohol Gen Dev
System level description: growth regulation
Input
e.g. cell
growth
saGD
WGD
Output
iaGD
BGD
“stationary”
cell
density
“log”
“lag”
time
e.g.
smell
System level description: growth regulation
Input
saGD
WGD
ON
e.g. log
growth
iaGD
BGD
OFF
“stationary”
cell
density
“log”
“lag”
time
wintergreen
smell
System level description: growth regulation
Input
saGD
WGD
OFF
e.g.
stationary
growth
iaGD
BGD
ON
“stationary”
cell
density
“log”
“lag”
time
banana
smell
System level description: growth regulation
Input
saGD
WGD
OFF
e.g.
stationary
growth
iaGD
BGD
ON
OsmY is a promoter that is only active during stationary phase
System level description: growth regulation
Input
saGD
WGD
OFF
e.g.
stationary
growth
iaGD
BGD
ON
OsmY
OsmY+ inverter
Stationary phase
Log phase
1
0
0
1
System level description: growth regulation
Input
saGD
WGD
OFF
e.g.
stationary
growth
iaGD
BGD
ON
System level description: Chassis modification
Input
saGD
WGD
OFF
e.g.
stationary
growth
iaGD
BGD
ON
eliminate
natural
indole
production
System level test: gas
chromatographs
E. coli
no methyl salicylate
[no peak at 9.1
minutes]
E. coli + precursor
(SA) + WGD
methyl salicylate
MW = 152
O
O
OH
System level test: uninformed
experimental subjects??