Figure S1: Microarray validation and concordance with Real Time results (A) Relative gene expression analysis 24h following aphid infestation. The graph.
Download ReportTranscript Figure S1: Microarray validation and concordance with Real Time results (A) Relative gene expression analysis 24h following aphid infestation. The graph.
Figure S1: Microarray validation and concordance with Real Time results (A) Relative gene expression analysis 24h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-Ct values were significantly different from the calibrator (p< 0.01; Student’s t-test). (B) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale (A) (B) 30 ** R² = 0,77 15 10 6 ** 4 2 ** ** ** * Fold change Microarray Relative Quantification (RQ) ** 20 20 15 10 5 - 0 I c II 1 1 0 F n2 /ER RLK Y 4 Inh Inh PR i K 2 m i N AP WR Gene 25 P4 apA L 0 5 10 15 20 Fold change Real-time PCR 25 Figure S2: Microarray validation and concordance with Real Time results (C) Relative gene expression analysis 48h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-Ct values were significantly different from the calibrator (p< 0.01; Student’s t-test). (D) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale. (D) (C) 50 ** 70 ** 40 30 20 10 ** 6 ** 4 ** 2 Fold change Microarray Relative Quantification (RQ) 60 R² = 0,95 50 40 30 20 10 - 0 I c 1 II 0 1 F n2 /ER RLK Y 4 Inh Inh PR i K 2 m Ni AP WR Gene 60 P4 apA L 0 20 40 60 Fold change Real-time PCR 80 Figure S3: Microarray validation and concordance with Real Time results (E) Relative gene expression analysis 96h following aphid infestation. The graph displays the relative quantity (RQ) of each target gene in infested plants relative to the calibrator control plants. Asterisks indicate that the 2-Ct values were significantly different from the calibrator (p<0.01; Student’s t-test). (F) The graph displays the concordance between microarray fold change and Real Time RQ values on a linear scale (F) 80 ** 35 ** 60 40 20 6 ** * 4 ** 2 0 Fold change Microarray Relative Quantification (RQ) (E) * I c II 1 1 0 F n2 /ER RLK Y 4 Inh Inh PR i K 2 Nim AP WR Gene P4 apA L 30 R² = 0,75 25 20 15 10 5 -5 (5) 15 35 55 Fold change Real-time PCR 75