Transcript Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42 A Log2(Normalised signal) 8 y = 1.0047x - 9.5772 R = 0.9675 2-2 -4 -6 -8 Log2[RNA.

Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-42
A
12
Log2(Normalised signal)
10
8
y = 1.0047x - 9.5772
6
2
R = 0.9675
4
2
0
-2
0
5
10
15
20
25
-4
-6
-8
Log2[RNA copies/ng total RNA]
B
40
38
36
34
y = -1.0205x + 42.617
Ct
32
2
R = 0.9988
30
28
26
24
22
20
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-81
A
10
8
Log2(Normalised signal)
6
y = 1.0389x - 10.155
R2 = 0.9671
4
2
0
-2
0
5
10
15
20
25
-4
-6
-8
-10
Log2 [RNA copies/ng total RNA]
B
42
40
38
36
34
Ct
32
y = -0.9946x + 40.515
30
2
R = 0.9992
28
26
24
22
20
18
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-84
A
16
14
Log2(Normalised signal)
12
10
y = 0.9772x - 5.8678
R2 = 0.9639
8
6
4
2
0
-2 0
5
10
15
20
25
-4
-6
Log2 [RNA copies/ng total RNA]
B
40
38
36
Ct
34
32
y = -0.9693x + 41.49
30
R = 0.9986
2
28
26
24
22
20
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-95
A
12
Log2(Normalised signal)
10
8
y = 0.957x - 8.8564
R2 = 0.9712
6
4
2
0
-2
10
5
0
20
15
25
-4
-6
-8
Log2[RNA copies/ng total RNA]
B
42
40
38
36
y = -1.0022x + 44.939
Ct
34
2
R = 0.9992
32
30
28
26
24
22
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-99
A
14
12
Log2(Normalised signal)
10
y = 1.0095x - 6.6213
R2 = 0.9626
8
6
4
2
0
-2 0
5
10
20
15
25
-4
-6
-8
Log2 [RNA copies/ng total RNA]
B
40
38
36
34
Ct
32
y = -1.0052x + 41.88
30
2
R = 0.9984
28
26
24
22
20
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-113
A
14
12
Log2(Normalised signal)
10
y = 1.0248x - 6.6933
R2 = 0.9993
8
6
4
2
0
-2
0
5
10
15
20
25
-4
-6
Log2 [RNA copies/ng total RNA]
B
40
38
36
34
Ct
32
y = -1.02x + 42.406
30
2
R = 0.9974
28
26
24
22
20
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25
Additional File 2: Linear range of ERCC detection (A)
microarray (B) RT-qPCR platforms: ERCC-171
A
12
10
Log2(Normalised signal)
8
y = 1.0696x - 10.718
R2 = 0.9993
6
4
2
0
-2 0
5
10
15
20
25
-4
-6
-8
-10
Log2[RNA copies/ng total RNA]
B
44
42
40
38
36
y = -0.9714x + 44.299
Ct
34
2
R = 0.9981
32
30
28
26
24
22
0
5
10
15
20
LogPCR Efficiency[RNA copies/ng total RNA]
25