Molecular biology (Zoo-342)

Dr. Mikhlid Hamed Almutairi Associate Professor Medical Molecular genetics

Department of Zoology , College of Science King Saud University P.O. Box 2455, Riyadh 11451 Saudi Arabia Office number: 67 بأ , Ground floor Emails: [email protected]

[email protected]

يريطملا دماح نب دلخم : روتكدلا ةيبطلا دعاسم ذاتسأ ةيئيزجلا ةثارولا

دوع س زمرلا كلملا ةعماج ،ضايرلا ،مولعلا ، 2455 ةيلك ،ناويحلا ملع مسق مقر ديربلا 11451 قودنص يديربلا ةيدوعسلا ةيبرعلا ةكلمملا يضرلأا رودلا ، 67 بأ : بتكملا مقر [email protected]

: ينورتكللاا ديربلا [email protected]

Aims of the Molecular Biology course

         To study the properties of genetic material.

To understand the structure of DNA and its organization in chromatin.

To understand the structure of RNA and its types.

To understand the of DNA replication.

To understand the key features of gene.

To study the gene expression: Transcription in prokaryotic cells.

To study the gene expression: Transcription in eukaryotic cells.

To study the gene expression: Translation in prokaryotic cells.

To study the gene expression: Translation in eukaryotic cells.

12 13 14 15 5 6 7 8 9 10 11 Time table for weekly planning for Molecular Biology Lectures 1 2 3 4 Dates

16/4/1436 23/4/1436 30/4/1436 7/5/1436 14/5/1436 21/5/1436 28/5/1436 2/6/1436 13/6/1436 20/6/1436 27/6/1436 4/7/1436 11/7/1436 18/7/1436 25/7/1436

Syllabus view

Required properties of genetic material Structure of DNA and RNA DNA replication The eukaryotic chromosome and chromatin organization Centromeres and telomeres Changes in chromosome structure and number Exam 1 Half term break holidays Gene expression Prokaryotic transcription: The process Eukaryotic transcription Posttranscriptional modification in eukaryotes Prokaryotic translation Eukaryotic translation Exam 2

Assessment

 Lecture  2 lecture exams (25%)    Attendance, participation and homework (5%) Final exam (40%) 70% of total grade  Laboratory  30% of total grade

1) 2) 3)

Required properties of a genetic material:

It must contain information that control the synthesis of protein and function.

It must be able to self-replicate.

It must be located in the chromosomes, which had been found to carry traits from one generation to the next.

1) 2)

Types of genetic materials:

DNA: is the genetic material in most organisms, such as humans, animals, plants, viruses, and bacteria.

RNA: in some viruses, RNA serves as the genetic material.

1) 2) Types of nucleic acids DNA (deoxyribonuclic acid) RNA (ribonuclic acid)

The structure of nucleoside and nucleotide   Nucleoside= nitrogen base + sugar Nucleotide= nitrogen base + sugar + phosphte

The structure of the four nucleotides

A polymer of nucleotides

The Watson-Crick model

Hydrogen bonds

Polarity of the DNA strand