Institute of Microbiology Chinese Academy of Sciences •Microbial Resources •Applied & Industrial Microbiology •Pathogenic Microbiology Advisory Committee Academic Committee International Advisory Panel Academic Degree Committee National Advisory Panel Development Committee Center.

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Transcript Institute of Microbiology Chinese Academy of Sciences •Microbial Resources •Applied & Industrial Microbiology •Pathogenic Microbiology Advisory Committee Academic Committee International Advisory Panel Academic Degree Committee National Advisory Panel Development Committee Center.

Slide 1

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 2

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 3

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 4

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 5

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 6

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 7

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 8

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 9

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 10

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 11

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 12

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You


Slide 13

Institute of Microbiology
Chinese Academy of Sciences
•Microbial Resources
•Applied & Industrial Microbiology
•Pathogenic Microbiology

Advisory Committee
Academic Committee

International Advisory Panel

Academic Degree Committee

National Advisory Panel

Development Committee

Center for
Microbial Resources

Center for Agri. Biotech.
Co-op with Inst. Genetics &
Develop. Biol., CAS

State Key Lab of
Microbial Resources
CAS Key Lab of
Syst. Mycol. & Lichenol.

Center for Environ. Biotech.

Center Microb. Metab. Eng.

Center for Microbial
Genome Research

Center for
Molecular Immunology
Center for
Molecular Virology

Center for Energy &
Industrial Biotechnology

Co-op with Beijing
Genomics Inst., CAS

Culture Collect. Center
Microbial Analy. Center

High-throughput
Screening Platform

Tech. Support Platform
Information Center

National Microbial Engineering Center
Administrative System

Developmental Strategy
 Construct Modern

Biotechnology
Platforms
 Human Resources
 Co-op with Industry

 International Co-op

Broad International Co-operation

MultiGenome Access Technology
for Industrial Catalysts
(MGATech)
An EU - China Collaboration

Review of FP5
in IMCAS
European Commission Fifth Framework RTD Programme
Quality of Life and Management of Living Resources
Key Action 3: The Cell Factory
RTD Priority 3.3.4: Identification and Sustainable Use of
Metabolic and Genetic Diversity as a Source of New Valuable
Products

MultiGenome Access Technology for Industrial Catalysts
An EU – China Collaboration

European Commission Fifth Framework RTD Programme
Thematic Programme : Quality of Life and Management
of Living Resources
Key Action 3 : The Cell Factory

Hydrothermal outbreak, upper site small pool
Rehai thermal area
Overview

RTD Priority 3.3.4 : Identification and Sustainable Use of
Metabolic and Genetic Diversity as a
Source of New Valuable Products
Project Number: QLK3-CT-2002-01972

Novel genetic methods will be developed for accessing gene products derived from Chinese hot springs
and salt lakes to which we have unique access in a collaboration with the Chinese authorities. These
Chinese extreme environments are of outstanding significance as microbial ecosystems with unique
chemistries. The sites harbour largely unexplored microbial genetic resources that will be harnessed as
sources of new and valuable enzymes.
Gene libraries reflecting the microbial biodiversity at these exceptional sites will be employed for the
identification and production of valuable new enzymes for sustainable applications in biotechnological
processes and consumer products. This special collaboration, which is the first microbial biotechnology
collaboration between the EU and China, will provide substantial opportunities for the development of
European and Chinese biotechnology.

Pearl Spring – Rehai thermal area

Objectives






Sampling algal mat at large basin
Langpu geothermal area

Obtain material from a range of unique Chinese hypersaline and
thermal sites
Survey microbial biodiversity, and establish and archive a
collection of novel microbes
Develop methods for the efficient isolation and recovery of
microbial genes from these sites
Identify and produce high added value new biomolecules derived
from these sites and assess these new biomolecules for
commercial exploitation

Deliverables and Achievements
• Sample Acquisition: The mounting of expeditions and collection of samples/nucleic acids from
seldom accessed thermal and hypersaline sites. – The first expedition to the Yunnan Province
(Rehai area) was completed in March 2003 and the second expedition to Inner Mongolia will take
place in September 2003.
• Gene Acquisition: We will recover microbial genes from a variety of samples, some partially
processed on site . There has been good progress with the isolation of DNA from geothermal sites
visited during the first sampling expedition and we have begun to generate bacterial gene libraries
derived from these sites. The first gene library derived from “Higher Microbes” (i.e. fungi, algae
and protozoans) has been constructed from a hot spring algal mat.
• Organism Acquisition: We will generate and archive culture collections of microbes from these
sites. The first joint paper by Consortium members has been submitted for publishing approval.
• Enzyme Acquisition: We will detect and assess industrially-relevant enzymes from the genetic
resources derived from these expeditions and technologies.
The Consortium

Hydrothermal outbreak pool
Rehai thermal area

•Prof. Bill Grant
- University of Leicester, UK

•Prof. Antonio Ventosa
- University of Sevilla, Spain
•Prof. Don Cowan
- University of the Western Cape, South Africa
•Prof. Ma Yanhe
- Institute of Microbiology, Chinese Academy of Sciences
•Prof. Brian Jones
- Genencor International BV, The Netherlands

The Sampling Team

Contacts

Coordinator: Prof. Bill Grant,
EU Scientific Officer: Dr. Indridi Benediktsson
University of Leicester, UK
[email protected]
[email protected]

极端微生物实验室

Extremozyme genes
• DNA library
• Metagenomic
DNA Libraries
• cDNA Library
High thoughput screening
-Mannanase
-Amylase
-Xylanase
Lipase
Catalase
…….

Design of an Integrated Environmental
Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of
Agricultural Wastes in Paper Industry
An EU - China Collaboration

Review of FP4
in IMCAS

European Commission Fourth Framework RTD Programme
Thematic Programme:Basic Natural Resources
Project number:ERBIC18CT980284

Design of an Integrated Environmental Clean Industrial Fungal Process Integrating
Water Management for the Valorisation of Agricultural Wastes in Paper Industry

Objectives:
1.Water pollution prevention: Development of a fungal enzyme
process using mainly laccases for improving annual plant kraft pulp
bleachability.
2.Water pollution treatment and recycling: Design of a fungal
process for effluent detoxification and decolourization in order to
facilitate the subsequent biological treatment and water recycling.
European Commission Fourth Framework RTD Programme
Thematic Programme : Basic Natural Resources
Project Number: ERBIC18CT980284
Coordinator: Prof.Dr. Jean-Claude Sigoillot
Institut National De La recherche Agronomique
[email protected]
The Consortium: -DR.GUIGAN FANG
CHINESE ACADEMY OF FORESTRY
-DR.HEINZ JELLER
ANDRITZ AG, AUSTRIA
-DR.LIN GUO
INSTITUTE OF MICROBIOLOGY, CAS

Activities:
To achieve these objectives, the proposed project has
been constructed with an integrated multidisciplinary
approach combining complementary Chinese and
European industrial partners including small enterprises in
close association with academics originating from both
countries.

Ongoing International Cooperative Projects
•Design new chromegens

Oxoid-Fisher Limited,UK
•Biodiversity and conservation of important bio-resources
Royal Botanic Gardens, Kew, UK
•Biodiversity of mycorrhyzal bacteria in forests of China and UK
Oxford University,UK
•Acidophilic actinomycetes as a source of new natural products
University of Newcastle, UK

IMCAS look forward to establishing the
long-term cooperation with EU

An EU – China
Collaboration

Thank You