Diffusion and Osmosis Start Quit TABLE OF CONTENTS Diffusion Osmosis Living Cell Osmosis Quit Diffusion and Osmosis DIFFUSION Dialysis Equipment Equipment Set Up Results Quit Diffusion and Osmosis Diffusion (Dialysis Equipment) glass dish dialysis tubing glucose test strips closures pipet Quit Diffusion and Osmosis Initially,
Download ReportTranscript Diffusion and Osmosis Start Quit TABLE OF CONTENTS Diffusion Osmosis Living Cell Osmosis Quit Diffusion and Osmosis DIFFUSION Dialysis Equipment Equipment Set Up Results Quit Diffusion and Osmosis Diffusion (Dialysis Equipment) glass dish dialysis tubing glucose test strips closures pipet Quit Diffusion and Osmosis Initially,
Diffusion and Osmosis Start Quit TABLE OF CONTENTS Diffusion Osmosis Living Cell Osmosis Quit Diffusion and Osmosis DIFFUSION Dialysis Equipment Equipment Set Up Results Quit Diffusion and Osmosis Diffusion (Dialysis Equipment) glass dish dialysis tubing glucose test strips closures pipet Quit Diffusion and Osmosis Initially, the dialysis tubing is filled with a super saturated glucose + starch solution Starch Water dialysis tubing Glucose continue animation Quit Diffusion and Osmosis The filled dialysis tubing is placed in a dish with a solution of iodine and water Iodine Water Starch Water Glucose The dialysis tubing membrane is selectively permeable continue animation Quit Diffusion and Osmosis Diffusion (Equipment Set Up) DIALYSIS TUBING starch, glucose and water iodine and water Quit Diffusion and Osmosis In DIFFUSION, particles travel from an area of high concentration to an area of low concentration As particles migrate through a membrane in this way, movement is described to be traveling “down a concentration gradient” Quit Diffusion and Osmosis When particles migrate through a selectively permeable membrane some particles are able to pass through but not others Quit Diffusion and Osmosis There is a GLUCOSE gradient across the membrane Iodine Water NONE HIGH Starch Water Glucose GLUCOSE molecules fit through the membrane and travel “down a concentration gradient” to the outside of the dialysis tubing … testing indicates GLUCOSE now in dish Quit continue animation Diffusion and Osmosis There is a IODINE gradient across the membrane HIGH Iodine NONE Starch Water Glucose Water IODINE molecules fit through the membrane and travel “down a concentration gradient” to the inside of the dialysis tubing … testing indicates IODINE now in dialysis tubing as Starch turns PURPLE Quit continue animation Diffusion and Osmosis There is a STARCH gradient across the membrane NONE Iodine HIGH Starch Water Glucose Water However, STARCH molecules cannot fit through the dialysis tubing membrane … water in dish is not PURPLE Quit continue animation Diffusion and Osmosis There is a WATER gradient across the membrane Iodine Water HIGH LOW Starch Water Glucose WATER molecules fit through the membrane and travel “down a concentration gradient” to the inside of the dialysis tubing … dialysis tubing is much fuller than before Quit continue animation Diffusion and Osmosis Diffusion (Results) dialysis tubing in iodine and water Quit Diffusion and Osmosis OSMOSIS Osmometer Equipment Equipment Set Up Results Quit Diffusion and Osmosis OSMOSIS (Osmometer Equipment) cup animal disc membrane bell straw rubber band cover pipet Quit Diffusion and Osmosis ASSEMBLING THE OSMOMETER straw inserted into narrow end of bell cup with lid bell opened at both ends selectively permeable membrane stretched across wide end of bell continue animation Quit Diffusion and Osmosis FILLING THE OSMOMETER The position of the solution in the bell is marked The bell is filled with a super saturated lactose solution (lactose and water) The cup is filled The solution is dyed to make it visible in one half full of water the bell continue animation Quit Diffusion and Osmosis A super saturated solution has a relatively low water concentration because of a VERY HIGH solute concentration The osmometer solution has a large amount of lactose solute dissolved in a small amount of water Quit Diffusion and Osmosis The osmometer is assembled continue animation Quit Diffusion and Osmosis Osmosis (Equipment Set Up) fluid level super saturated lactose and water solution water Quit Diffusion and Osmosis Diffusion of water takes place through the membrane. Water is pulled from an area of high concentration (in the cup) to an area of low concentration (in the bell) OSMOMETER continue animation Quit Diffusion and Osmosis As water diffuses through the membrane OSMOMETER “down the gradient” from the cup into the bell the overall fluid level rises up the straw continue animation Quit Diffusion and Osmosis The membrane is selectively permeable OSMOMETER the lactose solute particles because of their larger size are prevented from migrating through the membrane and rise up the straw along with the water continue animation Quit Diffusion and Osmosis Osmosis (Results) fluid level osmometer solution rises up the straw water is pulled in through selectively permeable membrane Quit Diffusion and Osmosis LIVING CELL OSMOSIS Elodea Before NaCl Application 400x Elodea Before NaCl Application 1000x Elodea After NaCl Application 400x Elodea After NaCl Application 1000x Quit Diffusion and Osmosis Elodea Before NaCl Application 400x Quit Diffusion and Osmosis Elodea Before NaCl Application 1000x cell wall cell membrane chloroplasts continue animation Quit Diffusion and Osmosis The cell is initially in an isotonic solution Water within the cell keeps the cell membrane against the cell wall 100% water 100% water 100% water Chloroplasts are evenly spread throughout the cytoplasm continue animation Quit Diffusion and Osmosis Elodea 1000x The cell is placed in a hypertonic salt solution continue animation Quit Diffusion and Osmosis When the cell is placed in a hypertonic solution there is a salt gradient across the cell wall and membrane Water goes “down the gradient” from a high water concentration inside of the cell ... 90% water 100% water water 10% salt Hypertonic Solution water water water 90% water 10% salt Hypertonic Solution to a low water concentration outside of the cell continue animation Quit Diffusion and Osmosis Elodea After NaCl Application 400x continue animation Quit Diffusion and Osmosis The rigid cell wall is porous and remains intact ... but without water inside of the cell, the cell membrane is pulled from the cell wall water water Hypertonic Solution Hypertonic Solution water water Chloroplasts are draw in with the cell membrane and collect in the middle of the cell Quit continue animation Diffusion and Osmosis Elodea After NaCl Application 1000x cell membrane cell wall chloroplasts Water is pulled out of cell through the cell wall into hypertonic solution The cell membrane crenates pulling chloroplasts into a small bunch The cell membrane separates from the cell wall End Quit Diffusion and Osmosis