Apoptosis Necrosis and Apoptosis Apoptosis Morphological Morphological Necrosis Biochemical Apoptosis Biochemical DNA ladder Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin.
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Transcript Apoptosis Necrosis and Apoptosis Apoptosis Morphological Morphological Necrosis Biochemical Apoptosis Biochemical DNA ladder Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin.
Apoptosis
Necrosis and Apoptosis
Apoptosis Morphological
Morphological
Necrosis Biochemical
Apoptosis Biochemical
DNA
ladder
Agarose gel
electrophoresis of DNA
isolated from
promyelocytic leukemia
cells (HL-60) were either
untreated (Lane 1) or
treated with 0.5 mg/ml
actinomycin D (Lane 2).
DNA Markers (M) were
electrophoresed as a
base pair reference.
nucleosome
FACS DNA
Anexin V
Anexin V
Necrosis physical
Apoptosis physical
Physical significance
3. Detection of Apoptosis
• Morphological
• DNA electrophoresis
• DNA断裂的原位末端标记
TUNEL测定法,Terminal deoxynucleotidyl
transferase(TdT)-mediated dUTP end
labeling,末端脱氧核苷酸移换酶介导的
dUTP缺口末端标记测定法
• Flowmetery
3.Regulation of Apoptosis
⑴ Genes for proliferation
c-myc
ras
⑵ For survival
bcl-2
c-kit
⑶ proliferation inhibitors
p53
Rb
⑷ death promotors
Bax
ICE
4.p53 and apoptosis
2002 Press Release
Laureates
Ap-overview