Transcript COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR.

COMPARATIVE STUDY BETWEEN THE
EFFECT OF HOMEOPATHIC AND
ALLOPATHIC MEDICINES IN
EXPERIMENTALLY INDUCED PATHOLOGY IN
LABORATORY ANIMALS
ETUDE COMPARATIVE DE L’ACTION DE
MEDICAMENTS HOMEOPATHIQUES ET
ALLOPATHIQUES SUR UNE PATHOLOGIE
EXPERIMENTALE CHEZ DES ANIMAUX DE
LABORATOIRE
Authors:
Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU,
Dr. Gabriela MARCUS, student Adriana COJOCARU
University of Medicine and Pharmacy Targu-Mures
ROMANIA
Aim
• To assess the effect of homeopatic
remedies in laboratory animals in
placebo controled studies
• Do homeopatic remedies act through
placebo effect?
Material and method
• Mice and rats from the bio-base (experimental
station) of the University of Medicine and
Pharmacy Targu-Mures
• Various induced experimental pathology
followed by treatment
– homeopathic,
– allopathic
– combined, homeopathic and allopathic
Experiments
The effect of the homeopathic remedies Arnica and
Staphysagria upon the healing time of the surgical incision
The antitrauma effect of the remedy Arnica in laboratory mice
The compensation of muscarinic blockade provoked by
atropine in laboratory animals with the homeopathic remedy
Belladonna
The effect of the homeopathic remedy Nux Vomica compared
with Diazepam on the manifestations of strychnine poisoning
in laboratory mice
Comparison of the anti-inflammatory effect of the homeopathic
remedy Apis and allopathic anti-inflammatory medicines in
experimental acute inflammation produced by formaldehyde
Homeopathic treatment of grade I burns in rats with Apis and
Belladonna
The effect of the homeopathic
remedies Arnica and Staphysagria
upon the healing time of the
surgical incision
The effect of the homeopathic remedies
Arnica and Staphysagria upon the
healing time of the surgical incision
• Previous studies made around the world with
homeopathic Arnica –contradictory results
• experimental studies with Staphysagria - missing
• What are the benefits in surgery?
• Our hypothesis – the need of administration of the
remedy early after surgery
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
• 75 Wistar rats, weight 250-270 g
• 5 groups of 15 animals
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Surgery technique
Step 1 - tranquilization
of the rats with ether
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Surgery technique
Step 2 - preparing the
surgery zone by
depilation and
disinfection
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Surgery technique
Step 3 - performing a
deep incision on the
external side of the
thigh, following the line
of the femur until the
appearance of the bone
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Surgery technique
Step 4 - skin suture in 3
separate points with
nonresorbable silk thread
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Treatment
Each rat was treated through a plastic probe, starting
immediately after surgery and daily administration
afterwards until the complete healing of the incision
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
Treatment
The homeopathic remedies were dissolved in water,
1 pellet in 1 ml water
• Group 1 - control group, sugar water as placebo,
• Group 2 - Arnica CH 7,
• Group 3 - Arnica CH 30,
• Group 4 - Staphysagria CH 7,
• Group 5 - Staphysagria CH 30.
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Material and method
• the length of the remaining incision was
measured each day
• the number of days necessary for the
complete healing was noted for each rat
The effect of the homeopathic remedies Arnica and Staphysagria upon the
healing time of the surgical incision
Results
The rats treated homeopathically, both remedies and
both dilutions, showed a shorter healing time (15-16
days) than the placebo group (21-22 days), with a
statistically significant difference (p<0,001, t-test)
Days for healing
25
Control
20
Arnica CH7
15
Arnica CH30
10
Staphysagria CH7
5
Staphysagria CH30
0
1
2
3
4
5
6
7
8
9
10 11 12 13 14 15 Rat
The antitrauma effect of the remedy
Arnica in laboratory mice
The antitrauma effect of the remedy
Arnica in laboratory mice
• 40 AKR2 white mice,
• 2 groups of 20 mice: control, Arnica
• Kocher clamp trauma at the level of the
posterior limb
– fractures
– swelling
– pain = protection of the affected limb, adynamia
• Assesment of the variation of the dorsoplantar
diameter
The antitrauma effect of the remedy Arnica in laboratory mice
Results
• Control group
– Persistent swelling more than 180 minutes
– adynamia
– Protection of the limb
• Arnica CH7 group
–
–
–
–
2
1,8
1,6
1,4
1,2
1
0,8
0,6
0,4
0,2
0
Moderate swelling, gone in less than 105 minutes (p < 0,001)
Good general state
Ability to use the affected limb, even fractured
normal activity
mm swelling (average/group)
0'
15'
30'
45'
mechanic trauma
60'
75'
Control
Arnica CH 7
90'
105'
120'
135'
150'
165'
180'
timp
The compensation of muscarinic
blockade provoked by atropine in
laboratory animals with the homeopathic
remedy Belladonna
The compensation of muscarinic
blockade provoked by atropine in
laboratory animals with the
homeopathic remedy Belladonna
• Pilocarpine – parasympathomimetic effect –
salivation in mice
• Atropine (Atropa Belladonna) - parasympatholytic
effect, blocking of the muscarinic receptors,
imposibility for pilocarpine to produce salivation
• Belladonna in homeopatic dilution – does it
compensate the atropinic intoxication?
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
80 de white mice AKR2 line divided in 4 groups:
1. Assessment of the salivation produced by pilocarpine
(administration of pilocarpine).
2. Assessment of the muscarinic receptors blockade by
atropine, given pilocarpine (administration of
atropine, then pilocarpine).
3. Assessment of supposed compensation of
muscarinic blockade of atropine by homeopathic
Belladonna CH7 (administration of atropine, then
Belladonna CH7, then pilocarpine).
4. Control group, assessment of physiologic salivation
(administration of placebo).
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
Step 1 – subcutaneous injection of Atropine 0,1%
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
Step 2 - Belladonna CH7 given by gastric probe
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
Step 3 – tranquilization with ethylic urethan intraperitoneally
1,5 g/kgc
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
Step 4 - subcutaneous injection with 1% clorhidric pilocarpine
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Material and method
The mice were put on filter paper
The area of salivary impregnation was measured each 10
minutes for 30 minutes
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Results
The physiologic salivation was low, nearly immeasurable on
the filter paper – group 4
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Results
Pilocarpine produces an abundant salivation (known
pharmacologic effect), assessed through the saliva spot area
on the filter paper – group 1
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Results
Atropine given before pilocarpine prevented the salivation,
which was very low, nearly immeasurable on the filter paper
(known pharmacologic effect) – group 2
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Results
If homeopathic Belladonna CH 7 is given after atropine and
before pilocarpine, the abundant salivation is present – group 3
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Results
The variation of salivation in the first 30 minutes
in the 4 groups
mm2 saliva spot
lot 1 pilocarpine
120
lot 3 atropine, belladonna CH7, pilocarpine
lot 2 atropine, pilocarpine
100
lot 4 physiologic salivation
140
(p<0,001)
80
60
40
20
0
0'
10'
20'
time
30'
The compensation of muscarinic blockade provoked by atropine in laboratory
animals with the homeopathic remedy Belladonna
Discussions
• In mice the homeopathic remedy Belladonna in CH 7 dilution
was able to give the atropinized organism the ability to be
stimulated by pilocarpine.
• It is possible, in theory, that the action of the homeopathic
remedy Belladonna CH 7 could be one of the following:
– it acted on the muscarinic receptors, determining them to
release the atropine molecules and become sensible
again to pilocarpine stimulation;
– it acted on the atropine molecules, determining them to
release the receptors on which they were bound;
– it acted on cellular level, determining possible alternative
ways for the de processing of the stimulus represented
by pilocarpine (activation of new receptors, alternative
biochemical or neuroendocrine ways)
The effect of the homeopathic remedy
Nux Vomica compared with Diazepam
on the manifestations of strychnine
poisoning in laboratory mice
The effect of the homeopathic remedy
Nux Vomica compared with Diazepam
on the manifestations of strychnine
poisoning in laboratory mice
• Stricnina (Strychnos nux-vomica L) - adynamia, tonicoclonic convulsions, hypersensitivity to sensorial stimuli,
deformation of visual and acoustic perceptions,
tachycardia, tachypnea, agitation and finally death.
• Diazepamul, benzodiazepină - tranquilizing, musclerelaxant, anticonvulsant
• Nux Vomica, Strychnos nux vomica seeds dilutions –
used in cases of convulsions, aggravated by external
stimuli, agitation, hypersensitivity to stimuli
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Material and method
3 groups of 20 white mice - experimental poisoning
- intraperitoneal injection of 1,5 mg/kg strychnine
– Group 1 - diazepam 5 mg/kg subcutaneous 1 hour
before strychnine
– Group 2 - Nux Vomica CH7 orally by plastic probe
immediatelly after strychnine
– Group 3 - control (placebo), physiologic serum
subcutaneous, sugar water by plastic probe
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Material and method
• Strychnine poisoning:
– adynamia
– tachycardia
– tachypnea
– agitation
– tonico-clonic convulsions
– death
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Material and method
We assessed:
• The time until convulsions
• The number of animals which had convulsions
• The time until death
• The number of animals which died
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Results
The time until convulsions
• 180-390 sec Placebo group
• 240-570 sec Diazepam group
• 660-1080 sec homeopathic Nux Vomica group
Placebo
Diazepam
Nux Vomica CH7
0
200
400
600
800
1000 seconds until convulsions
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Results
• Convulsions present 100% in the Placebo and
Diazepam group
• 70% of mice had convulsions in the
homeopathic Nux Vomica group
convulsions
no convulsions
Placebo
Diazepam
Nux Vomica CH7
0
5
10
15
20
number of mice
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Results
• Placebo group mice died in 240-610 sec
• Diazepam treated mice lived longer, 370-685 sec
• Homeopathic Nux Vomica treated mice showed the
longest survival, 605-1150 sec
Placebo
Diazepam
Nux Vomica CH7
0
200
400
600
800
1000 survival, seconds
The effect of the homeopathic remedy Nux Vomica compared with Diazepam
on the manifestations of strychnine poisoning in laboratory mice
Results
• All the mice (100%) in the Placebo and
Diazepam group died
• Homeopatically treated mice with Nux
Vomica died in 80%
died
survived
Placebo
Diazepam
Nux Vomica CH7
0
5
10
15
20
Comparison of the anti-inflammatory
effect of the homeopathic remedy Apis
and allopathic anti-inflammatory
medicines in experimental acute
inflammation produced by formaldehyde
Comparison of the anti-inflammatory
effect of the homeopathic remedy Apis
and allopathic anti-inflammatory
medicines in experimental acute
inflammation produced by formaldehyde
• Formaldehyde – mastocyt reaction histamine
• Apis - swelling, redness, pain, warmth
• Hydrocortisone Hemisuccinate (HHS) steroidal anti-inflammatory
• Diclofenac - nonsteroidal anti-inflammatory
Comparison of the anti-inflammatory effect of the homeopathic remedy Apis
and allopathic anti-inflammatory medicines in experimental acute inflammation
produced by formaldehyde
Material and method
• 70 white mice – local swelling in the
posterior limb by subcutaneous
formaldehyde 0.5%
• Dorsoplantar diameter was measured
before formaldehyde and then each 15
minutes
Comparison of the anti-inflammatory effect of the homeopathic remedy Apis
and allopathic anti-inflammatory medicines in experimental acute inflammation
produced by formaldehyde
Material and method
7 groups of 10 mice
1. Hydrocortisone Hemisuccinate
2. Diclofenac
3. Apis CH 7
4. Apis CH 15
5. Hydrocortisone Hemisuccinate and Apis CH 7
6. Hydrocortisone Hemisuccinate and Apis CH 15
7. Control group, placebo
Comparison of the anti-inflammatory effect of the homeopathic remedy Apis
and allopathic anti-inflammatory medicines in experimental acute inflammation
produced by formaldehyde
Results
• Local signs of
inflammation
– redness
– swelling
– motor impairment
– agitation
– protection of the limb
Comparison of the anti-inflammatory effect of the homeopathic remedy Apis
and allopathic anti-inflammatory medicines in experimental acute inflammation
produced by formaldehyde
Results
Dorsoplantar diameter evolution in time (average values)
mm swelling
Control - Placebo
Diclofenac
HHS
Apis CH 15
HHS + Apis CH 7
Apis CH 7
HHS + Apis CH 15
0,7
HHS, Diclofenac
0,6
formaldehyde,
Apis
0,5
0,4
0,3
0,2
0,1
0
0'
15'
30'
45'
60'
75'
90'
105'
120'
135'
150'
165'
180'
timp
Homeopathic treatment of grade I
burns in rats with Apis and Belladonna
Homeopathic treatment of grade I
burns in rats with Apis and Belladonna
Material and method
• 3 groups of 10 rats
• Tranquilization with ether
• Immersion of posterior left limb in hot
water at 55°C for 20 seconds
Material and method
Grade I burns - edema and erithema
Material and method
Treatment by gastric probe:
• Group 1 - placebo as sugar water
• Group 2 - Apis CH 9
• Group 3 - Belladonna CH 9
Material and method
Assessment:
– The number of healed rats each day
– The average number of days necessary for the
complete healing for each group
Results
• Apis CH 9 group
showed the fastest
healing, followed by
the Belladonna CH 9
group
• The Placebo group
showed the longest
healing time
Placebo
Belladonna
CH 9
Apis CH 9
0
1
2
average, day for healing
3
4
Results
• Apis group healed in
the days 1-2, with 80%
rats in the first day
• Belladonna group
healed in the days 1-3
• Placebo group healed
in the days 2-4
12
Apis CH 9
Belladonna CH 9
10
Placebo
8
6
4
2
0
Day 1 Day 2 Day 3 Day 4
Final conclusions
Conclusions
(1/4)
• The homeopathic remedies Arnica and Staphysagria in the
dilutions CH 7 and CH 30, given early after surgery, had a
significant effect in the reduction of the healing time of the
surgical incision in rats
• The homeopathic remedy Arnica CH 7 fights the effects of
mechanic trauma in mice if given immediately after trauma
• In mice the homeopathic remedy Belladonna in CH 7
dilution was able to give the atropinized organism the
capability to be stimulated by pilocarpine, even if the
specific receptors were blocked
Conclusions
(2/4)
• Homeopatic Nux Vomica CH7 was significantly
more efficient than Diazepam in delaying and even
preventing the convulsions in the experimental
strychnine poisoning. The time until death was
significantly prolonged, some of the mice treated
homeopathically surviving the experiment
• The anti-inflammatory effect of the remedy Apis in
our experiments had a higher intensity than
Hydrocortisone Hemisuccinate and Diclofenac,
without being antidoted by them in combinations
• Apis CH 9 wad more efficient than Belladonna CH 9
in the treatment of grade I burns in rats, both being
more efficient than placebo
Conclusions
(3/4)
In all our experiments, the effect of homeopathic
remedies was objectively assessed, reproducible
and statistically significant
Homeopathy is different from placebo
Homeopathy acts quickly in acute
Homeopathy can be associated with allopathy
Conclusions
(4/4)
Homeopathic research must be extended
C'est la guerre!
Thank you for you attention