Transcript title - iGEM 2007

title
まりも？
• 自然界の毬藻形成
– いまの自分たちの「まりも」との共通点、大事
なエッセンスを抽出しよう！
• 私たちの「毬藻」
まりも？
Sticky Hand
+ Communication
+ Size control
Marimo
How it works?
まんが
Sticky Hands
+ Communication
+ Size control
Marimo
Sticky Hands
+ Communication
+ Size control
Marimo
Sticky Hands
Various systems….
Display
Affinity
Sticky Hands
FliC-Display
Explain flagella system
Sticky Hands
+ Communication
+ Size control
Marimo
Sticky Hands
His-Tags
Explain the chemistry
Sticky Hands
+ Communication
+ Size control
Marimo
Sticky Hands
His-Tagged FliC
Construction
Construct
Sticky Hands
+ Communication
+ Size control
Marimo
Swarm Plate; positive
Data
Sticky Hands
Affinity Binding
How to do the assay
procedure
Sticky Hands
+ Communication
+ Size control
Marimo
Beads Binding
Bar graph
ただしネガコンに難ありかも？
Sticky Hands
Metal driven aggregation
Procedure
Construct
Sticky Hands
+ Communication
+ Size control
Marimo
Aggregation
Beads を使ってでも
何が何でも
無理矢理凝集だ！
Sticky Hands
+ Communication
+ Size control
Marimo
Communication modules
?
AHL SYSTEM, overview
Sticky Hands
+ Communication
+ Size control
Marimo
AHL construct
Receiver
Construction
Construct
Receiver
+/- AHL
(Chemical induction)
+/- AHL
Data
+/- AHL
Plate
AHL construct
Receiver
Construction
Construct
Sticky Hands
+ Communication
+ Size control
Marimo
Sender + Receiver
+/- AHL
Data
System
AHL-FliC/His
PLUX FliC
PBAD FliC
Sticky Hands
+ Communication
+ Size control
Marimo?
Swarm? YES/ NO
+/- AHL
Data
TEST
実験
Sticky Hands
+ Communication
+ Size control
Marimo?
Binding-to-gether
+/- AHL
Data
possible source of
problems
Visualに問題点を洗い出す
Sticky Hands
+ Communication
+ Size control
Marimo?
System
Super sender
Sticky Hands
+ Communication
+ Size control
Marimo?
[AHL]
Pathway and construct
Switching threshold
ここでmetK plus METのconstructの話とdataをみ
せちゃう
System
Super receiver
Switching threshold
Sticky Hands
+ Communication
+ Size control
Marimo?
Cynthia論文
System
Super receiver, result
Sticky Hands
+ Communication
+ Size control
Marimo?
construct
Switching threshold
DATA
Sticky Hands
+ Communication
+ Size control
Marimo?
System
AiiA #1
PLUX FliC
AHL
Switching threshold
AiiA
Sticky Hands
+ Communication
+ Size control
Marimo?
System
AiiA #2
PLUX FliC
cI
AiiA
PL
Switching threshold
AHL
どーなる？
Summary
Crazy dreams
Thanx