Chapter 7 The Microscope
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Transcript Chapter 7 The Microscope
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
1 7-
1. Ocular lens
2.Revolving nosepiece
3. Lower power
objective
4. Medium power
objective
5. High power objective
6. Stage clips
7. Diaphragm
8. Light source
(illuminator)
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
2 7-
9. Eyepiece
10. Arm
11.Stage
12. Coarse
Adjustment
13. Fine Adjustment
14. Base
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
3 7-
1. Ocular Lens: Lens within the eyepiece
2. Revolving Nosepiece- contains the
objective lens
3. Objective Lenses: lens directly over
specimen; low, medium and high power
4.Stage- supports slide
Stage clips- secures slide on stage.
5. Diapraghm (condenser)- focuses light on
specimen
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
4 7-
6. Light source (illuminator)- source of artificial
light
7. Arm: upright structure which supports
microscope
8. Base: Supports microscope
9. Body tube: allows light can pass through to go to
each lens
10. Coarse Adjustment: focus an image under low
power
11. Fine Adjustment: focuses an image under
medium and high power
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
5 7-
Compound Light Microscope
How is it used?
Light passes through translucent specimen to view
internal structures.
When is it used?
On translucent or thin specimens.
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
6 7-
The Compound Microscope
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
7 7-
The Comparison Microscope
How is it used?
Two independent objective lenses joined
together by an optical bridge to a common
eyepiece lens.
Can view two different items
simultaneously
When is it used?
Firearms examination
Trace evidence (hair, fibers, etc)
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
8 7-
The Stereoscopic Microscope
How is it used?
Two monocular compound microscopes
Three-dimensional image of a specimen
Use both eyepiece lenses
When is it used?
Evidence not requiring very high
magnification (10x–125x)
Microscopic examination of big, bulky items
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
9 7-
The Microspectrophotometer
How is it used?
Spectrophotometer coupled with a
light microscope.
Can simultaneously obtain the visible
absorption spectrum or IR spectrum
of the material being observed
When is it used?
Trace evidence, paint, fiber, and ink
evidence.
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
10 7-
The Scanning Electron Microscope
How is it used?
(SEM) bombards a specimen with a beam of
electrons instead of light
Produces a highly magnified image from
100x to 100,0000
Depth of focus 300X better than optical
systems at similar magnification
Bombardment of the specimen’s surface
with electrons
Produces x-ray emissions
Characterize elements present in the material
under investigation
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
11 7-
When is it used?
Get a very magnified image
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
12 7-
Dental Plaque
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
13 7-
Bread Mold
Aspergillus sp.)
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
14 7-
Penecillium sp.
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
15 7-
SEM: Hair and Hair follocles
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
16 7-
SEM: hafnium nitride interface
coating on carbon fibers
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
17 7-
Dust Mite
Family Reunion
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
18 7-
Important Terms
Field of View: area of specimen that can be
seen after its magnified
Depth of Focus: thickness of specimen
entirely in focus under microscope
Polarizer: devise that permits the passage
of light waves vibrating in only one plain.
Parafocal: can change objective lens and
field of view will remain in focus
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
19 7-
Monocular: microscope with one
eyepiece
Binocular: microscope with two
eyepieces
Virtual Image: image that is
magnified (cannot see it directly)
Real image: image seen directly
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
20 7-
5. What happens to a compound microscopes field of
view when you increase the magnification?
Field of view decreases
6. When using a comparison microscope why is it
important to closely match the optical characteristics?
Ensure both specimens are seen at equal magnification
and with minimal but identical lens distortions.
7. What is the most frequently used and versatile
microscope found in the crime lab? List 3 reasons why
this type is so widely used
Stereoscopic microscope because of wide field of view,
great depth of focus, large working distance
CRIMINALISTICS
An Introduction to Forensic Science, 9/E
By Richard Saferstein
PRENTICE HALL
©2007 Pearson Education, Inc.
Upper Saddle River, NJ 07458
21 7-