Transcript Slide 1

NON-INVASIVE
MEASUREMENT METHOD
OF SKIN CONDITIONS
USING DERMALAB®
COMBO
Presented by:
Asst. Prof. Dr. Hazrina Ab Hadi
BPharm, Hons., MSc. Drug Delivery, PhD Pharmaceutics
Department of Pharmaceutical Technology
Kulliyyah of Pharmacy
International Islamic University Malaysia
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
Skin structure and function
Epidermis
Sebaceous
gland
Dermis
Hair follicle
Eccrine
sweat gland
Hypodermis
Blood vessel
Schematic illustration of cross-section of human skin
(adapted from Servier Medical Art)
DermaLab® Combo
TEWL open chamber probe
•Principle of vapor diffusion gradient
•Sensor inside - to avoid any air currents and direct light.
•The TEWL measurement is expressed as g/m2/hr.
Hydration pin probe
• Conductance principle is applied
• Measure skin moisture.
• Conductivity - to measure the water binding capacity of the
stratum corneum.
Skin color probe
• Based on the light absorption by the skin
• The color measurement is based on the principle of
reflectance spectrophotometry.
• This probe has an optics design in order to ensure minimal
sensitivity to ambient light condition.
Elasticity suction probe
• Measure the necessary force needed to lift the skin
• Provide a light weight probe
• Skin elasticity of subjects is determined by refraction time (R)
once the negative pressure is released.
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
Aims and Objectives
• To evaluate biophysical parameters (TEWL, hydration,
melanin content and elasticity) of the skin using Dermalab
Combo
• To compare skin parameters between genders
• To identify possible factors that affect functional state of the
skin based on the skin condition measurements
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
Methodology
• 100 volunteers (IIUM students)
• Age: 18 – 27
• Consent Form + simple questionnaire
(demographic profile and daily
activities)
• Acclimatize to ambient T
• Measurement on the volar forearm
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
Results and Discussion 1/9
TEWL in different gender
12
10
TEWL
8
Female
6
Male
4
2
0
Gender
Results and Discussion 2/9
Use of moisturiser vs TEWL
16
14
12
TEWL
10
Use
8
Not Used
6
4
2
0
Use of Moisturizer
Results and Discussion 3/9
Average hydration between genders
205
200
195
Hydration
190
185
Female
180
Male
175
170
165
160
Gender
Results and Discussion 4/9
250
150
More 1L
Less 1L
100
50
300
0
Amount of Water Intake
250
Hydration
Hydration
200
200
Used
150
Not used
100
50
0
Use of Moisturizer
Results and Discussion 5/9
Average melanin content between genders
80
70
Melanin Content
60
50
Female
40
Male
30
20
10
0
Gender
Results and Discussion 6/9
Type of activity vs Melanin content
70
60
Melanin Content
50
40
Outdoor
Indoor
30
20
10
0
Type of Activity
Results and Discussion 7/9
Sunblock usage vs Melanin content
70
60
Melanin Content
50
40
Used
Not Used
30
20
10
0
Sunblock Usage
Results and Discussion 8/9:
Skin Elasticity between genders
490
480
Elasticity
470
460
Female
Male
450
440
430
420
Gender
Results and Discussion 9/9:
Use of moisturiser vs elasticity
Used
600
500
Elasticity
400
Not Used
Used
300
Not
Used
Used
200
100
0
Use of Moisturizer
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
Conclusions
• Biophysical parameters of the skin vary among different
genders. The differences may be due to individuals daily
activity, use of skin care products as well as environmental
factors.
• The use of DermaLab® Combo in this experiment provides a
reliable measurements for TEWL, hydration, pigmentation
and elasticity. Thus, this baseline reading can be used for
further research study such as comparison of skin care
products and assessment of skin care efficacy.
OUTLINE
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Background
Aims and Objectives
Methodology
Results and Discussion
Conclusions
References
References
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Alexander H, Cook T. Variations with age in the mechanical properties of human skin in vivo. J Tissue Viability 2006;16:6–
11.
Bouwstra JA, Dubbelaar FE, Gooris GS, Ponec M: The lipid organisation in the skin barrier. ActaDermVenereolSuppl 2000;
208: 23–30.
Choi, J. W., Kwon, S. H., Huh, C. H., Park, K. C., &Youn, S. W. (2013). The influences of skin visco‐elasticity, hydration
level and aging on the formation of wrinkles: a comprehensive and objective approach. Skin Research and
Technology, 19(1), e349-e355.
DermaLab® Combo. Cortex Technologies, Denmark; (2011). Retrieved Jun 8, 2014, from www.cortextechologies.dk
Ehlers, C., Ivens, U. I., Møller, M. L., Senderovitz, T., &Serup, J. (2001). Females have lower skin surface pH than men. Skin
Research and Technology,7(2), 90-94.
Elias, P. M., & Choi, E. H. (2005). Interactions among stratum corneum defensive functions. Experimental
dermatology, 14(10), 776-776.
Ishikawa, T., Ishikawa, O., &Miyachi, Y. (1995). Measurement of skin elastic properties with a new suction device (I):
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Kleesz, P., Darlenski, R., &Fluhr, J. W. (2011). Full-body skin mapping for six biophysical parameters: baseline values at 16
anatomical sites in 125 human subjects. Skin pharmacology and physiology, 25(1), 25-33.
Maddodi, N., Jayanthy, A., &Setaluri, V. (2012). Shining light on skin pigmentation: the darker and the brighter side of
effects of UV radiation†.Photochemistry and photobiology, 88(5), 1075-1082.
Proksch, E., Brandner, J. M., & Jensen, J. M. (2008). The skin: an indispensable barrier. Experimental dermatology, 17(12),
1063-1072.
Rosado, C., Pinto, P., & Rodrigues, L. M. (2009). Assessment of moisturizers and barrier function restoration using dynamic
methods. Skin Research and Technology, 15(1), 77-83.
Taieb, M., Gay, C., Sebban, S., &Secnazi, P. (2012). Hyaluronic acid plus mannitol treatment for improved skin hydration
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Thank you for your attention!