Transcript Microbial Detection Arrays - University of Colorado Boulder

Microbial Detection Arrays
Jeff Childers
Dave Miller
Elizabeth Newton
Ted Schumacher
Shayla Stewart
Steven To
Charles Vaughan
Sameera Wijesinghe
Critical Design Review
December 5th, 2006
Aerospace Senior Projects
University of Colorado – Boulder
Advisors: Dr. Forbes and Dr. Maslanik
Customers: BioServe and Tufts University
Briefing Overview
• Overview of Objectives and
Requirements
• System Architecture
• Prototype Results
• Mechanical Design Elements
• Electrical Design Elements
• Software Design Elements
• Integration, Verification, and Test Plan
• Project Management Plan
• Appendices
2
Objectives
• Component of larger project
– Future Mars astrobiology mission from
BioServe/Tufts University/JSC
– Astrobiology objective: electrochemical sensing of
metabolic activity
– Three components: biology (JSC), sensors
(Tufts), instrument hardware (CU)
• MiDAs team objective: instrument hardware
component
– Design/build integrated field instrument with
meaningful biological and spaceflight constraints
– Validate key functions to enable field research
– Extends proof-of-concept from lab to field
• Raise TRL from 1-3 to 4-5
3
TRL Objective
https://www.spacecomm.nasa.gov/spacecomm/programs/technology/default.cfm
4
Deliverables
• Field-ready unit (TRL 4-5)
• Test data that verifies requirements
• Operational manual for use
• Document proposing design solutions
to further raise the TRL (to 6-7)
5
Requirements Overview
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
Water tubing not shown
Samples placed in
autoclaves
Autoclaves heated to 121°C
and held for 15 minutes
Autoclaves cooled to 20°C
and held for 24 hours
Process may be repeated up
to 3 times
Valves opened
Water pumped into
autoclaves
Sample flushed into reaction
chambers
Inoculation sample added to
test chamber
Environmental chamber
maintained between 4°C and
37°C
Mixers stir sample and water
Sample is tested for 14 days
6
Requirement Refinement
• Complete autonomy no longer primary goal
– Increased reliance on experimenter to open valves
and deliver inoculation sample
– Instrument will not provide its own power
• Reason:
– Change at request of customer – trades autonomy
for reliability in field instrument
– Autonomy adds expense, complexity, and failure
modes without proving key concepts or raising TRL
– Autonomy options will be included in design
document
– Key components maintained in field instrument
7
Mars/Earth Comparison
Theoretical Mars Mission
MiDAs Earth Based Apparatus
Receive low power from Rover
Receive low power from external source
Receive startup command from uplink
Press power button
Rover opens Autoclave lid
Person opens Autoclave lid
Rover inputs sample
Person inputs sample
Rover closes Autoclave lid
Person closes Autoclave lid
Autoclave cycle begins
Autoclave cycle begins through SW run command
Rxn chamber environment controls begin
Rxn chamber environment controls begin
Valve opens
Person opens valve
Water flushes sample out of autoclave
Water flushes sample out of autoclave
Valve closes
Person closes valve
Mixing begins
Mixing begins through SW run command
DAq begins
DAq begins through SW run command
Inoculation sample added
Person adds inoculation sample
DAq runs for 14 days
DAq runs for 14 days
Data downlink from rover to satellite to Earth
Data stored on-board, transfer to PC
8
System Architecture (External)
Dimensions:
18” x 18” x 15”
(46 cm x 46 cm
x 39 cm)
9
10” (25 cm)
15” (39 cm)
System Architecture (Internal)
16” (40 cm)
10
Mass Analysis
Insulation
9.83g
2 Pumps
127g
2 Autoclaves
1890g
Water Chamber
132g
2 Valves
1450g
Tubing 396g
4 TECs 720g
Environmental
Chamber 656g
2 Reaction
Chambers 173g
Chassis
1150g
2 Mixers
95.8g
CPU and DAq
(not shown)
292g
Internal Mass: 7.10kg (15 lbs)
Total Mass: 13.90 kg (30 lbs)
Sensors
(not shown)
10.0g
11
Experiment Timeline
Finish
Start
0
30 s
1.5 hr 3 hr
27 hr
25.5 hr
51 hr
49.5 hr
75 hr
73.5 hr
Soil
Soil
2 weeks
+ 27 to 75 hr
Autoclave
Reaction
t= t066,7,8,9
sample manually
1 Insert
2
3
4
5
___A
B___
**optional
Heater A:
Heater B:
Can repeat
two more
Cooler
A:
Cooler
B: times
S Cycle B: F*
-F*
S
-Cycle A: **
A
Soil
B
Soil
12
Electrical Overview
KEY
13
Autoclave Prototype
• Concerns:
– Low power heating
– Seals
•304 Stainless Steel
•Height = 2.25 in.
•Inner Diameter = 1.5 in
•LabView
•External temperature sensor
•Internal pressure sensor
14
Prototype Thermal Analysis
• Steady state
2W energy loss
• Heater on flat
area
• Large thermal
gradient
15
35
140
30
120
temperature (deg C)
pressure (psi)
Autoclave Prototype Results
25
20
15
10
5
0
100
80
60
40
20
0
0
20
40
60
80
100
120
140
time (min)
• Results:
– 121 C for small 12W strip
heater, higher pressure than
expected
– Very uneven heating
– Seals held
0
20
40
60
80
100
120
140
time (min)
•Conclusions:
− 3 smaller strip heaters
evenly spaced
−TEC used only for cooling
−O-ring seals were effective
−Melamine insulation was
effective
16
Mixing Prototype
• Ultrasonic
– Frequency function of tip length
– 18 kHz not feasible
• Magnetic
– May disrupt electrochemical sensors
• Pending tests by Tufts
• Mechanical
– No off-the-shelf impeller options
– Custom impeller designed
17
Mixing Prototype Results
Results:
• Too much slip with impeller to use
motor
– Had to rotate impeller manually
• Sample developed air bubbles
• Flour-like consistency  very slow
settling time
• Sediment remains on bottom of
chamber
Conclusions:
• Fluid movement around sides easily
maintained
• Need cross-bar near the bottom
• Can maintain colloidal solution for
several minutes without continuous
mixing with 10-micron grains
18
Sample Transport Prototype Results
Results:
•
•
•
•
Conclusions:
¾” tubing did not transport sample • 1” tubing
30% soil transported when dry
• Water added to move sample
95% soil transported when wet
Autoclaving did not affect soil
19
consistency
Autoclave Drawings
• 316 stainless steel
• Height = 2 in. with flat
sides = 1.6 in. x 1.6 in.
• Wall thickness = 0.125 in.
• Inner Diameter = 1.5 in.
tapered
Lid
Sensor
Ports
O-ring
Body
Bottom
View
Valve
Interface
1” diameter
20
Reaction Chamber Drawing
•
•
•
•
Ultem 1000
Height = 5.2 in. (13.19 cm)
Diameter = 1.6 in. (3.95 cm)
Wall thickness = 0.197 in. (0.5
cm)
• Soil transport pathway = 1.0 in.
(2.5 cm)
• Cap to support mixing shaft
• 20 sensor ports
– 12 electrochemical sensors
– 7 multi use ports
– 1 temperature sensor
Cap
Sensor ports
Impeller
Motor
Reaction Chamber
with Mixer and Cap
21
Autoclave Stress Analysis
• Autoclave technique:
– 121 C with steam to aid heat flow
– 15 psi above atmosphere for saturated steam at 121 C
• Thin wall pressure formulas:
– Minimum thickness = 0.011 in. while actual used = 0.125 in.
– Critical pressure for 0.125 in. is 20 kpsi
• Seals:
– Regular threads alone will not seal
– O-ring compression seals made of silicone for high temperature and
pressure
• Conclusions:
– O-ring seals are effective
– Temperature of chamber is regulated and heater has limited heating
power
– Pressure relief valve added to 10-32 port on lid
22
Electrical System
• Power supply is 12V
• Power conditioning is added to give cleaner power
• 5V power will be used to run sensors because of
voltage stability
+5VDC
LM340-XX
+12VDC
2
T
U
O
N
Breaker
Circuit
?
C
Cap
.1uF
Zener
D
N
D
N
G
G
F
u
0
0
1
Cap
D
1
.22uF
?
D
?
C
Earth
Cap
D
N
G
1
?
C
V
2
1
+
SW-SPST
I
3
Amps
Supply
Power
5
Switch
off
On
U
Voltage regulator
?
AC-DC converter
Power supply
23
1
TEC
AC
2
TEC
AC
1
TEC
RC
2
TEC
RC
2
Control
RC
2
TEC
1
TEC
Autolcave
x
3
x
2
x
3
x
2
x
3
Autoclave
1
Control
RC
x
x
2
x
3
2
x
3
x
2
heater2
Autoclave
LED's
x
2
x
2
x
2
x
6
– TECs, mixers and
LEDs
1
Heater1
Autoclave
Output
Analog
x
9
x
input
Digital
x
6
8
1
Board
Switch
Input
Analog
x
0
1
x
0
2
Sensors
• Sensors will run
constantly
• Switchboard controls
power to:
Mixer
1
Control
Mixer
x
3
x
2
– Pumps, TECs and
mixers
2
• The DAQ card can
proportionally control:
Mixer
2
Control
Mixer
x
2
Computer
Distribution
Power
Supply
Power
Sensors and Control
24
Software Timeline
Finish
Start
0
30 s
1.5 hr
Insert sample
Turn on
27 hr
Heating complete
Autoclave A
Autoclave B
2 weeks
+ 27 to 75 hr
Done
Turn
Valve
Pumping complete
Water pump A & B
Done
Reaction Chamber
Autoclave control
Reaction control
1.
User turns on program
1.
2.
Autoclave A begins heating
User turns valves open
and beings program
3.
At 121˚C Autoclave A holds
for 15 min
2.
Turn on pumps for 25
sec (at 1mL/sec flow
rate)
4.
Autoclave A begins cooling
and Autoclave B begins
heating
3.
Turn on Reaction
Chamber control
5.
Autoclave A finishes cooling
6.
Autoclave B finishes cooling
7.
Program notifies user
autoclave has completed
25
Assembly Flow Diagram
Chassis
Autoclave
chambers
(x2)
Body
Assembly
Reaction
Chamber
Envir.
Cap
Assembly
Body
Cap
Pressure
Seal
Temp &
Pressure
Sensors
Insulation
Strip
Heater
Reaction
chamber
(x2)
TEC
Assembly
TEC
Body
Assembly
TEC
Assembly
Body
Assembly
Power
Supply
DAQ
Embedded
CPU
Interface
Power
Supply
Interface
Body
TEC
Body
Temp. &
pressure
Sensors
Body
Insulation
Heat Sink
Insulation
ISE
Package
Temp &
Pressure
ISE
Package
Temp &
Pressure
Sensors
Strip
Heater
Power
supply
ISE
Package
Body
Assembly
Heat Sink
Mixer
Assembly
Make
Buy
Reagent
H2O
Chamber
Temp &
Pressure
Sensors
Sensors
Thermal
Control
Motor
TECs
Bearing
Strip
Heater
Gears
DAQ
Impeller
Peristaltic
Pump
26
Functional Test Plan
Thermal
Control
Autoclave
Sample
Transport
Reaction
Chamber
Thermal
Control
TEC
Strip
Heater
Butterfly Valve
Sample
Consistency
TEC
Motor
Mixing
Impeller
DAQ &
Control
Collection
& Storage
Command
Interface
Software
Heat from -10°C to 121°C
Hold for 15 min
Cool to 20°C
Repeat 3 times
Transport 90% of sample
when reagent water
pumped through
Maintain temperature
between 4°C and 37°C
Maintain fluid movement
around sides; Maintain
minimal sedimentation on
sides and bottom of chamber
Collect & store data from
each sensor
Receive commands from SW
Provide caution, warning,
status signals
27
Verification and Test Plan
Reaction
Chambers
Autoclaves
Reagent H2O
Chamber
Sample
Transport
Data Acquisition
& Control
Power
Temperature
4°C – 37°C
Thermistor in environmental chamber
Pressure
1 psi differential
Pressure sensor in environmental chamber
Mixing
Small sedimentation,
fluid flow @ sensors
Visual/Video verification
Temperature
≥121°C
Thermistor inside autoclave chamber through cap
Pressure
≥15 psi
Pressure sensor inside autoclave chamber through cap
Sample sterility
No microbial life in sample
Petri dish testing with bacteria and medium (BioServe)
Containment
Solid & liquid form
Thermistor inside autoclave chamber through cap
≤50mL (±5% accuracy)
Time-based flow rate in peristaltic pump (controlled flow)
< 60°C
Thermistor inside water chamber
Aseptic delivery
Sterile swabbing of wet surfaces, culture test
Collection &
Storage
Collected & stored for
entire experiment
DAQ storage capability analysis
Caution, Warning,
Status
Provide status, caution
& warning signals
Testing LabView command software with set max
temperature and shut-off abilities
Nominal
Consumption
≤ 30W
Peak
Consumption
≤ 30W for ≤ 30 sec
Delivery
Sterilized sample
Inoculation
Power model for all parts,
measurement through multimeter in circuit
28
Risk Assessment
Medium
Low
Severity
High
•Sample
transport
•Autoclave
•Mixing
•Water
transport
•DAQ
•Reaction
Chamber Thermal
Control
•Budget
Low
•Machining
Time
Medium
Probability
High
29
Work Breakdown Structure
MiDAs
Project Management
Design
Document
Fabrication
Verification and
Testing
Project Manager
Elizabeth Newton
Lead Fabrication
Engineer
Dave Miller
Design Engineer
Chuck Vaughan
Systems
Engineer
Shayla Stewart
Assistant Project
Manager
Ted Schumacher
Assistant
Fabrication
Engineer
Sameera
Wijesinghe
Design
Engineer
Jeff Childers
Software
Engineer
Steven To
Assistance as
Needed from
Team
Assistance as
Needed from
Team
Assistance as
Needed from
Team
30
Schedule
31
Overall Budget
ITEM
PART NUMBER
QUANTITY
PRICE ($)
86145K27
1 (24"x48"x2")
HK5544R33.1L12B
7
$ 236.95
CP-0.8-127-06L
4
$ 106.40
HX6-201-L-M
4
$
SA1-RTD
6
$ 300.00
PX139
4
$ 340.00
-
2
$ 00.00
8686K81
1 (24”X2” rod)
$ 155.00
316 Stainless steel
89325K673
2 (12”X2.5” rod)
$ 300.00
Aluminum
89015K53
2 (48”X48”X0.0625”)
$ 230.00
Bearing
6384K44
1
$
7.41
Rotary-Shaft 1/4" Ring Seal
9562K41
1
$
3.15
Pumps
P625/275.133
2
$ 690.00
Motors
1224
2
$ 600.00
4820K31
2
$ 173.27
DMM-37X-AX
2
$ 480.00
Embeded CPU
MOPSlcdLX
1
$ 450.00
Mixer Controller
PA75CC
2
$
WTC3243
4
$ 348.00
TOTAL
$4540.86
THERMAL CONTROL
Insulation (Melamine)
Strip Heater
Thermoelectric Cooler (TEC)
Heat Sink
$
49.48
46.20
SENSORS
Temperature
Pressure
ISE Package (18/pkg.)
MECHANICAL
Ultem 1000
Butterfly Valve
COMPUTER/DAQ
DAQ
Thermoelectric Controller
25.00
32
Resources and Facilities
• BioServe Laboratories
–
–
–
–
–
–
Matching funds
Spare/small parts
Machine shop
Temperature-controlled testing environment
Wet/Biological lab
Clean room
• Aerospace Department
– Machine Shop
– Electronics Shop
33
Conclusions
• Project feasible
• Team has necessary expertise, time
and resources
• Risk mitigated through prototyping
• Can increase overall TRL
34
References
1. Cengel, Yunus. Introduction to Thermodynamics and Heat Transfer.
McGraw-Hill. University of Nevada, Reno. 1997
2. Gilmore, David. Spacecraft Thermal Control Handbook. Aerospace
press. El Segundo, California. 2002
3. Mankins, John C. “Technology Readiness Levels.” April 6, 1995.
http://ipao.larc.nasa.gov/Toolkit/TRL.pdf.
4. www.dimondsystems.com
5. www.kontron.com
6. www.matweb.com
7. www.mcmaster.com
8. www.melcor.com
9. www.minco.com
10. www.omega.com
11. www.sonaer.com
35
Presentation Appendix
1. Title Page
2. Briefing Overview
3. Objectives
4. TRL Objective
5. Deliverables
6. Requirements Overview
7. Requirement Refinement
8. Mars/Earth Comparison
9. System Architecture (External)
10. System Architecture (Internal)
11. Mass Analysis
12. Experiment Timeline
13. Electrical Overview
14. Autoclave Prototype
15. Prototype Thermal Analysis
16. Autoclave Prototype Results
17. Mixing Prototype
18. Mixing Prototype Results
19. Sample Transport Prototype Results
20. Autoclave Drawings
21. Reaction Chamber Drawings
22. Autoclave Stress Analysis
23. Electrical System
24. Sensors and Control
25. Software Timeline
26. Assembly Flow Diagram
27. Functional Test Plan
28. Verification and Test Plan
29. Risk Assessment
30. Work Breakdown Structure
31. Schedule
32. Overall Budget
33. Resources and Facilities
34. Conclusions
35. References
36
Drawing Tree
37
Drawing Tree (continued)
38
Mechanical Drawing Tree
Autoclave Body
40
Autoclave Cap
41
Autoclave Bottom
42
Thermoelectric Cooler (TEC)
43
Heat Sink
44
Reaction Chamber
45
Reaction Chamber Cap
46
DC Motor
47
Impeller
48
Reaction Chamber Environment
49
Reaction Chamber Environment
Side Door
50
Peristaltic Pump
51
Pump Mount
52
PharMed Tubing
53
DAq
54
Embedded CPU
55
Chassis
56
Chassis Top
57
Chassis Front Interface
58
Electrical Schematic Tree
59
1
TEC
AC
2
TEC
AC
1
TEC
RC
2
TEC
RC
1
Mixer
2
Mixer
2
Control
2
1
TEC
Autolcave
x
3
x
2
x
3
x
2
x
TEC
x
3
2
Autoclave
1
Control
x
RC
x
3
2
x
3
x
2
RC
1
Control
Mixer
x
3
x
2
2
Control
Mixer
heater2
Autoclave
LED's
Heater1
Autoclave
x
2
x
2
x
2
x
4
Output
Analog
x
9
x
input
Digital
x
3
0
2
Board
Switch
Input
Analog
x
0
1
x
0
2
Sensors
x
2
Computer
Distribution
Power
Supply
Power
Electrical Schematic
60
D
N
G
D
N
G
F
u
0
0
1
Cap
Zener
D
1
.1uF
.22uF
?
C
?
D
Earth
Cap
Cap
D
N
G
1
?
C
?
C
V
2
1
+
Breaker
Circuit
SW-SPST
T
U
O
N
I
2
3
Supply
Power
Amps
5
+5VDC
LM340-XX
+12VDC
Switch
off
On
?
U
Power System
61
Diagram
Sensor
D
Sig
G
5
6
D
N
D
N
R
4
N
VS
+
3
G
2
G
PX139
D
N
G
1
AP2
7
8
PX139
9
0
1
R
1
1
2
1
Sig
VS
+
Res3
AT2
K
1
K
1
0
1
K
6
R
D
N
G
P
R
K
0
1
Q
A
D
Res3
PX139
AT1
5
R
R
K
1
K
0
1
Res3
Sig
VS
+
T
A
4
R
K
1
K
0
1
Res3
D
N
G
AP1
T
C
3
R
PX139
K
1
K
0
1
Res3
R
T
T
2
R
Sig
VS
+
K
1
K
0
1
Res3
T
R
1
R
P
A
+5VDC
Sensor Schematics
62
Sig
D
N
G
V
5
+
AT2
Sig
D
N
G
V
5
+
AT1
Sig
D
N
G
V
5
+
D
V
5
+
T
N
G
Sig
C
Sig
V
5
+
T
D
N
G
A
Sig
D
N
G
Sig
V
5
+
D
N
G
Sig
V
5
+
D
N
G
V
5
+
T
T
Sig
Sig
D
N
G
D
N
G
V
5
+
V
5
+
Sig
D
N
G
T
R
V
5
+
Sig
Sig
D
N
G
D
N
G
V
5
+
V
5
+
Sig
P
R
D
N
G
Sig
V
5
+
D
N
G
Sig
V
5
+
D
N
G
V
5
+
AP2
Sig
Sig
D
N
G
D
N
G
V
5
+
V
5
+
Sig
D
N
G
AP1
V
5
+
Sig
Input
DAQ
D
N
G
V
5
+
P
A
Sensor Wire Harness
63
Motor
Pump
M
?
B
5
2
6
P
System
Control
CLR
D
N
G
8
MI
+
G
D
N
D
N
G
2
SCI
+12VDC
+5VDC
D
K
0
1
4
PG
2
H
R
-V(ref)1
9
N
5
PG
3
+V(ref)
8
G
7
MI
-
6
PI
DC
Pump
Peristaltic
7
LED1
5
G
PA75CC
LED2
K
5
K
5
K
5
K
D
N
1.5K
1.5K
1.5K
1.5K
Mixer1
Vs
-
M
B
IAA
+
1
IAA
O
4.9K
4.9K
P
R
P
R
?
33.3K
K
0
1
33.3K
K
0
A
IAB
+
A
Vs
+
B
A
O
D
6
N
5
G
1
H
R
4
D
3
N
2
G
D
N
G
1
Computer
I
R
I
R
Control
Motor
K
1
K
1
Res3
Res3
R
R
33.3K
33.3K
L
L
4
1
C
8
9
1
1
1
1
7
0
1
2
3
6
5
4
3
2
1
I
8
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R
1
1
1
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C
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I
R
6
5
4
3
2
1
F
R
I
R
D
N
G
WTC3243
WTC3243
CONTROLLER
TEC
CONTROLLER
TEC
PA75CC
K
0
2
K
0
2
B
A
O
D
N
G
Vs
+
RBIAS
RBIAS
IAB
+
Mixer1
Vs
-
M
?
B
IAA
+
IAA
-
K
0
1
K
0
1
A
A
O
D
N
G
Control
Motor
Thermistor
IHC1
Thermistor
IHC2
K
1
K
1
Res3
Res3
F
R
I
R
Control Schematics
64
Motor
Pump
M
?
B
5
2
6
P
System
Control
CLR
8
MI
+
D
N
G
7
MI
-
6
PI
DC
5
PG
K
0
1
4
PG
3
+V(ref)
2
SCI
D
N
G
-V(ref)1
Pump
Peristaltic
1
2
3
4
5
6
7
8
9
+12VDC
Computer
D
N
G
D
N
G
K
5
K
5
K
5
K
5
1.5K
1.5K
1.5K
1.5K
4.9K
4.9K
P
R
P
R
33.3K
33.3K
I
R
I
R
R
R
L
L
1
C
8
9
1
1
1
1
7
0
1
2
3
6
5
4
3
2
1
8
9
4
1
1
1
1
1
7
C
0
1
2
3
4
6
5
4
3
2
1
WTC3243
WTC3243
CONTROLLER
TEC
CONTROLLER
TEC
K
0
2
K
0
2
RBIAS
RBIAS
K
0
1
K
0
1
Thermistor
IHC2
Thermistor
IHC2
Control Schematics continued
65
Sig
D
N
G
V
2
1
+
Pump
D
N
G
V
2
1
+
Heater2
D
N
G
V
2
1
+
Heater1
Sig
D
N
G
V
2
1
+
Sig
Pump
D
N
G
Sig
V
2
1
+
D
N
G
V
2
1
+
D
N
G
V
2
1
+
TEC4
D
N
G
Sig
V
2
1
+
D
N
G
Sig
V
2
1
+
D
N
G
V
2
1
+
TEC3
Sig
V
5
+
D
N
G
D
N
G
V
2
1
+
V
2
1
+
Sig
D
N
G
TEC2
V
2
1
+
V
5
+
V
5
+
D
N
G
D
N
G
V
2
1
+
V
2
1
+
V
5
+
TEC1
D
N
G
Sig
V
2
1
+
D
N
G
Sig
V
2
1
+
D
N
G
V
2
1
+
Mixer2
Sig
Sig
D
N
G
D
N
G
V
2
1
+
V
2
1
+
inputs
Control
Mixer1
Control Wire Harness
66
D
V
1
+
5
+
G
N
8
3
0
4
V
2
6
3
5
3
7
3
D
N
G
9
V
5
+
3
V
2
1
+
D
N
G
4
3
Distribution
Power
V
5
+
2
3
V
2
1
+
0
3
D
N
G
8
2
V
2
1
+
6
2
D
N
G
4
2
V
2
1
+
2
2
D
N
G
0
2
V
2
1
+
8
1
7
1
Sig
D
N
G
6
1
5
1
Sig
V
2
1
+
4
1
3
1
Sig
D
N
G
2
1
1
1
Sig
V
2
1
+
0
1
9
Sig
D
N
G
8
7
Sig
V
2
1
+
6
5
Sig
D
N
G
4
3
Sig
V
2
1
+
2
1
Sig
Items
Switched
Board
Switch
Output
Digital
Switch board
67
DAq Block Diagram
www.Dimondsystems.com
68
Embedded CPU
www.kontron.com
69
Software tree
AIn = Analog Input:
Acquires pressure and temperature data
DBit Out = Digital Bit Out: toggles output high or low to
control the switch board
Err Msg = Error message: displays error message if output
is not configured right
To Eng = Converts binary inputs from levels to voltage level
ToEngArray= Converts array of binary inputs to voltage level
= Autoclave temperature/pressure.vi
= Elapse Timer: Counts amount of time elapsed after specific case
= Time Delay: Waits specified time before taking next sensor data
= Write File: Writes data to measurement file
70
Software Prototype
71