Transcript ENZYMES - METU | Middle East Technical University

SSME-518
Instructional Technology and Material
Development
Assignment2
Presented by: Harika Özge Kızılsu
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Outline
Introduction to enzymes
How enzyme work
 Activation energy
Factors effecting enzyme activity
 Temperature
 pH
 Enzyme concentration
 Substrate concentration
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Introduction to enzymes
• Enzymes are usually proteins
• Enzymes are catalysts
• In general, names end with suffix “ase”
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Introduction to enzymes
Catalyst
Time for reaction
Yes
No
1 second
3 x 10 12 years
3 x 1012 years is 500 times the age of the earth!!!!
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Introduction to enzymes
Enzymes:
• increase the rate of a reaction
• not consumed by the reaction
• act repeatedly to increase the rate of reactions
• are often very “specific” for the substrate
Reactants also called “substrates” of enzyme
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How enzyme work
• Enzymes physically interact with their substrates
• Substrates bind to the enzyme’s active site
• Enzyme reaction path:
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How enzyme work
• Lock and Key Model
Substrate fits into an active site perfectly.
(key)
(lock)
click to see animation of model
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How enzyme work
• Activation energy is:
the energy required to start a reaction.
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Factors effecting enzyme activity
 Temperature
 pH
 Enzyme concentration
 Substrate concentration
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Temperature
Rate of reaction
36
0
10
20
30
40
C (optimum
temperature)
50
60
70
Temperature
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Rate of reaction
pH
0
1
2
3
4
5
6
7
8
9
pH
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Rate of reaction
Enzyme concentration
Substrate concentration
constant
Enzyme concentration
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Rate of reaction
Substrate concentration
Enzyme concentration
constant
Substrate concentration
click to learn more
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 Thank YOU 
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References
• http://fig.cox.miami.edu/~cmallery/255/255
enz/enzymology.htm
• http://ntri.tamuk.edu/cell/enzyme2.html
• http://www.animationfactory.com/animatio
ns/buildings/doors/6038e/
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