Transcript Principles and Procedures for Rearing Quality Insects

The Basics of Quality Control
for Insect Rearing
Norman C. Leppla
University of Florida
Department of Entomology
and Nematology
Leppla’s Career in
Insect Rearing
• University of Arizona- Insect
•
•
•
Rearing Research, 2 years
USDA, ARS- Insectary Management,
Florida & Texas, 17 years
USDA, APHIS- Methods
Development, Washington DC &
International, 7 years
University of Florida- Integrated
Pest Management, 10 years
Countries of Workshop Students
Educational
Background
Entomology
Agronomy
PhD
MS
Plant Health
BS
High School
0
On the Job
5
10
Number of responses
15
Maintaining the Quality
of Colonized Insects
• Collection of appropriate biotypes
• Colonization and strain development
• Rearing proficiency
• Colony management
• Optimization
• Strain replacement
I. Introduction
II. Colony Establishment and
Maintenance
II. A. Purposes for Rearing Insects
II. B. Types of Insect Rearing Systems
II. C. Options for Colonizing Insects
II. D. Maintaining the Quality of
Colonized Insects
II. A. Purposes for Rearing Insects
II. B. Types of Insect
Rearing Systems
Painted Lady
Monarch
Small Scale Rearing: Butterflies
Insect Rearing Facilities
Diet Preparation Building
General Rearing Building
State-of-the-Art Rearing Rooms
Medium-Scale Rearing:
Lepidoptera
Cabbage Looper
Corn Earworm
USDA, ARS Rearing
Keith Halein
Clarence Green
Jack Rye
Bill Fisher
USDA, ARS Rearing
Fred Adams
Fred Adams
Steve Carlyle
Annie Lorie
Insect rearing is never a
boring task; insects are
always doing something
interesting and pose new
challenges all the time
Punky
Rogers
USDA, ARS
Rearing
Mass Rearing: Medfly
Waimanalo, Hawaii
Metapa, Mexico
Honolulu, Hawaii
Nori Tanaka
II. C. Options for Colonizing
Insects
Peter Ebling
Insect Producer Database Mgr.
Great Lakes Forestry Centre
1219 Queen St. East
Sault Ste. Marie, ON P6A 2E5
[email protected]
(705) 541-5517
• World-wide listing35 insect & 21
nematode orders
• Data submitted by
sources
• Searchable
database
• Expand clients
• Adopt-a-colony
http://www.insect.glfc.cfs.nrcan.gc.ca
Field Collecting
Cabbage Loopers
Continuous Improvement of
Insect Rearing Capability
II. D. Maintaining the Quality
of Colonized Insects
Specifications- Requirements for
a product or service
Standards- The level of quality at
which a specification is written
Bonus: Learning How to Rear
High Quality Insects
•Apprentice in an insectary
•Network with other professionals
•Literature plus trial-and-error
•Visit other insectaries
•Reviews by experts
•Education and Training programs
First Training Program for
Insectary Managers
•Rearing Theory- Dietetics and nutrition,
laboratory colonization, production,
problems & solutions, quality assessment
& performance, future of insect rearing as
a scientific discipline
Rearing Practical- Biology, colony
maintenance, production management
Insectary Management- Insect rearing
management concept (IRM), planning,
staff management
•
•
Insect Diet & Rearing
Research, LLC
•Rearing news
•Research
•Consultation
•Educational programs
•Quality control
•Custom workshops
http://www.insectdiets.com
III. Monitoring Quality
III. A. Quality Control Criteria and
Standardized Tests
III. B. Sampling for Consistent
Quality
III. C. Production, Process and
Product Control
III. A. Quality Control Criteria
and Standardized Tests
•Quantity- Number of Pupae/Adults
•Size- Weight of Pupae
•Fecundity- Oviposition and Egg Hatch
•Rate of Development- Synchronization
•Adult Behavior- Flight, Longevity
•Field Performance- Achieve Purpose
III. B. Sampling for
Consistent Quality
Count= Measurement (X)
Mean= Sum/Number of Counts (X)
Variance= Sum of Counts - X2 (V)
Standard Deviation= Square Root of V (S)
III. C. Production, Process
and Product Control
IV. Evaluation and Management
IV. A. Structured Diagnostic
Procedures
IV. B. Quality Control versus
Methods Improvement
IV. C. Periodic Review versus
Crisis Review
IV. A. Structured Diagnostic
Procedures
IV. B. Quality Control versus
Methods Improvement
Quality Control
Monitor indicator variables
Evaluate multiple variables
Troubleshoot using QC data
Conduct evaluations rapidly
Goal is to restore stable production
Methods Improvement
Conduct evaluations methodically
Test one variable at a time
Use controlled experiments
Test results in the production system
Goal is to optimize production
•
•
•
•
•
•
•
•
•
•
IV. C. Periodic Review versus
Crisis Review
•
•
•
•
•
•
•
Terms of reference
Preparation
Seek to understand
Clarify
Reflect
Report
Feedback
V. Guidelines for
Quality Systems
•Policy, Planning and Administration
•Design Assurance and Change Control
•Control of Purchased Materials
•Production Quality Control
•User Contact and Field Performance
•Corrective Action
•Employees- Select, Train and Motivate
Global Quality Control
Programs
•Individual Companies
Customers
•International Standards
ISO 9000
ASTM
IOBC Guidelines
•The Marketplace
Quality Products
to facilitate and advance
cost-effective rearing of
high quality insects and
other arthropods in
support of biological
control and integrated
pest management
http://www.amrqc.org
Workshops of the IOBC,
WGQC (AMRQC)
1982 Gainesville, Florida
1984 Wadenswil, Switzerland
1986 Guatemala City, Guatemala
1988 Vancouver, Canada
1991 Wageningen, Netherlands
1992 Horsholm, Denmark
1993 Rimini, Italy
1995 Santa Barbara, California
1998 Cali, Colombia
2003 Montpellier, France
2007 Montreal, Canada
2010 Vienna, Austria
E. F. Boller and D. L. Chambers
E. F. Boller and D. L. Chambers
C. 0. Calkins
C. 0. Calkins
F. Bigler and J. C. van Lenteren
F. Bigler
M. Benuzzi and N. C. Leppla
R. F. Luck and N. C. Leppla
N. C. Leppla and T. R. Ashley
P. De Clercq, S. Grenier and
N. C. Leppla
S. Grenier and C. S. Glenister
P. De Clercq and T. A. Coudron
Improving Male Fruit Fly Performance
Abiotic environment
• Holding Conditions
• Release methods (knockdown, handling)
Biotic environment
• Juvenile hormone (Methoprine, fenoxycarb)
• Nutrients (sucrose, protein)
• Semiochemicals (methyl eugenol, citrus oil)
Insect Pest Control (IPC)
*Model
Business Plan for a Sterile
Insect Production Facility
http://www-naweb.iaea.org/nafa/ipc/index.
VI. Total Quality Management
Total
Quality
Management
in Insect
Rearing
Leppla’s Insect Rearing Quality
Control References
•Leppla, N. C. 2008. The basics of quality control
for insect rearing. In Principles and
Procedures for Rearing Quality Insects.
Mississippi State University.
Leppla, N. C. 2004. The Basics of Insect Rearing.
Encyclopedia of Entomology.
Leppla, N. C. 2003. Guidelines for quality control
of commercially produced natural enemies. In
Quality Control and Production of Biological
Control Agents, Theory and Testing Proc.
Leppla, N. C. 2002. Rearing of Insects.
Encyclopedia of Insects.
•
•
•
For more information or a copy of
this presentation please visit:
http://ipm.ifas.ufl.edu
Extension Resources, Presentations