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RosBREED Never Sleeps Integration & value of international partners Amy Iezzoni, Michigan State University Cameron Peace, Washington State University Primers work great! Info ready for our breeders Great! Try these new predictive primers Cool! Alright, I’m figuring out the functional alleles… Found a nice QTL for crispness! Cool! Outline of Presentation RosBREED and our international partners: an Introduction Attracting key international collaborators International collaborators become international partners Full integration and value of international partners RosBREED and our international partners: an introduction The RosBREED Project • PI: Amy Iezzoni, MICHIGAN STATE UNIVERSITY • 4 years, SEP 2009 – AUG 2013 • Funded by SCRI (USDA-NIFA’s Specialty Crop Research Initiative) • $14.4 M ($7.2 M SCRI, $7.2 M in-kind Partners) The RosBREED Project Mission We will create a dynamic, sustained program in research, infrastructure establishment, training, and outreach for developing and applying marker-assisted breeding based on improved knowledge of industry value and consumer preferences to accelerate and increase the efficiency of rosaceous cultivar release and successful cultivar adoption. We’re In It Together CROPS of the ROSACEAE FAMILY Demonstration Breeding Programs Challenge 1 QTLs found in experimental populations are rarely used for MAB – validation with breeding germplasm done rarely and ad hoc! Challenge 2 >63 Mil genetic data points will be generated in RosBREED from genome scans of apple, peach, and cherry! International Collaboration Solution World class Dutch stats & software team at Plant Research International/Wageningen University is a critical RosBREED subcontract Netherlands MSU local expertise International Collaborators Plant Research International, Netherlands East Malling Research, UK Andres Bello University, Chile CRA-FRU, Rome, Italy Plant & Food Research, New Zealand IASMA, Trento, Italy ARC, South Africa INRA (Bordeaux, Avignon, Angers), France RosBREED participant location + 2 Scientific Advisory Panel members (Spain, New Zealand) Attracting key international collaborators Attracting Key Collaborators • International collaborators were “attracted” by a common problem that needed a solution The Chasm The PROBLEM = The CHASM • “The Chasm” was not unique to the U.S. • Many leading groups on the Genomics side of the chasm were not in the U.S. – to be successful we had to involve these key groups • Discussions of how to bridge the chasm were ongoing (including international forums) for many years prior to our SCRI proposal Bridging the Chasm Bridging the Chasm Collaborators joined because… • RosBREED would provide solutions to a shared problem • Solving would be more efficient, faster, and cost effective if we all worked together • They supported the approach • They were comfortable with the leadership team – due to prior relationships International collaborators become international partners Collaborators vs. Partners • Collaborators and Partners work together towards common mutually agreed-upon goals • Partners, in partnerships, have legally binding agreements that define tasks and expectations The Funding Match • The fund-matching requirement of SCRI projects created opportunities in innovation and partnership • We required partners to make financial commitments and document expenditures for USDA audit • This is very time consuming. Therefore partners need to be very supportive and committed to the project Full integration and value of international partners Example 1: The SNP Summit Goal: Develop genome-scanning capability at medium-resolution (1 fully informative marker per 5 cM) for peach, apple, and cherry • What genotyping technology to use? • Which specific markers to include? • How can we do this most cost effectively? Example 1: The SNP Summit Step 1 Bring everyone together in person to develop strategies, obtain buy-in, and assemble international teams to do the work Include representation from Illumina, the company that would commercialize the SNP arrays The SNP Summit Example 1: The SNP Summit SNP Summit 13-14 November 2010, held with 5th International Rosaceae Genomics Conference in South Africa. RosBREED paid for our matching partners to attend Example 1: The SNP Summit Step 2 No formal presentations – only brainstorming for 2 days But, did have mandated outcomes, and everyone felt the pressure Example 1: The SNP Summit SNP Summit was a great success; - strategies agreed upon - crop teams given one month (!) to choose all SNPs - Illumina recognized us as an Ag Consortium and committed to commercialization of SNP arrays Example 1: The SNP Summit Met our 1-month goal SNP arrays available to world for purchase from Illumina from Dec 2010 – at much lower cost than if RosBREED had gone it alone SPECIAL EARLY ACCESS PRICING For 9000 SNPs Tier A Tier B Tier C Tier D Number of Samples (Total orders received for all Consortium members) <2016 2016+ 4032+ 6000+ Price per Sample (USD) $100 $75 $65 $60 Example 1: The SNP Summit And… Entire international Rosaceae genomics, genetics, & breeding community can continue to collaborate and share information – because identical genetic markers used Example 2: Strawberry Goal: Develop genome-scanning capability at medium-resolution (1 fully informative marker per 5 cM) for cultivated strawberry (octoploid) 56 chromosomes with much genetic redundancy Regarded as too scary to tackle by many scientists! Example 2: Strawberry • Undaunted, commitment made at SNP Summit • Word got out RosBREED not shying away from this challenge people joined • Four international groups donated key segregating populations for inclusion in replicated RosBREED reference germplasm set Increased chance of success – all major strawberry genetics teams working together, sharing germplasm and markers Example 2: Strawberry Populations imported from – France – Spain – Netherlands – UK Location of European institutions that provided key populations Example 2: Strawberry And… Two new U.S. partners joined RosBREED at their own expense, providing additional field plot locations and evaluation data Breeding Program Locations Vance Whitaker, University of Florida Phil Stewart, Driscoll’s Strawberry Associates Partnering strawberry breeders Example 2: Strawberry For genetic challenges, strategy developed through weekly conference calls Drs Dorrie Main & Cameron Peace Wash State Univ. Dr. Nahla Bassil, USDAARS Drs. Tom Davis & Hailong Zhang, Univ. New Hampshire Dr. Stephen Ficklin, Clemson Univ. Genomics Institute Drs. Cindy Lawley, Mark Hansen & Jill Orwick, Illumina Drs. Iraida Inc. Amaya & José F. Sánchez Sevilla, IFAPA-Centro de Churriana Dr. Eric van de Weg, Plant Research Int. Dr. Béatrice Denoyes-Rothan, INRA Dr. Dan Sargent, Istituto Agrario San Michele all’Adige Dr. Jasper Rees, ARC Example 2: Strawberry International partnerships grown well beyond initial proposal RosBREED began bridge building, and others joined Summary RosBREED tackles major scientific challenges that others share, and fills knowledge gaps Keys to attracting & benefiting from inclusion of international partners: - great ideas - communication - trust Summary Working together vastly increases accomplishments - greater conceptual breakthroughs by sharing ideas - further reach by standing on each other’s shoulders with shared datasets And of course someone, somewhere, is always working RosBREED never sleeps! Acknowledgements This project is supported by the Specialty Crop Research Initiative of USDA’s National Institute of Food and Agriculture MSU Amy Iezzoni (PD) Jim Hancock Dechun Wang Cholani Weebadde Univ. of Arkansas John Clark WSU Cameron Peace Dorrie Main Kate Evans Univ. of Minnesota Karina Gallardo Jim Luby Raymond Jussaume Chengyan Yue Vicki McCracken Nnadozie Oraguzie Oregon State Univ. Mykel Taylor Alexandra Stone Cornell Susan Brown Kenong Xu Clemson Ksenija Gasic Gregory Reighard Texas A&M Dave Byrne USDA-ARS Nahla Bassil Gennaro Fazio Univ. of CA-Davis Chad Finn Tom Gradziel Carlos Crisosto Plant Research Intl, Netherlands Univ. of New Hamp. Eric van de Weg Tom Davis Marco Bink