Transcript Astaxanthin – King of the Carotenoids

AstaREAL Astaxanthin:
®
Introduction
Nature’s Power to Human Health
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PR E S E NTAT I O N
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OUTLINE
What is Astaxanthin?
Why is it so potent?
Health Benefits
Regulatory
Marketing Astaxanthin
AstaREAL Bulk Product List
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ASTAXANTH I N BAC K G R O U N D
1938
Lobster
Present Day
Other Sources
Microalgae
Yeast
Bacteria
Higher Plants
Crustaceans
Fish
Birds
(Astacus gammarus) Discovered
by Nobel Laureate Dr Kuhn
(Haematococcus pluvialis)
(Phaffia rhodozyma)
(Agrobacterium aurantiacum)
(Adonis aestivalis)
(Euphasia supurba)
(Salmon) Typically 1mg per 200g
(Flamingo, Cockerel)
Microalgae 60,000 ppm Yeast 10,000 ppm Krill 300 ppm.
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ASTAXANTH I N I S PAR T O F TH E
CAROTENOID FAMILY
– Lutein
– Astaxanthin
-Carotene
OH
OH
• 732 known carotenoids*
• Humans cannot synthesise
carotenoids.
• Need to obtain carotenoids
from food.
• Less than 25 kinds
obtained from food.
• Basic carotene structure
• Xanthophyll Class
Lutein
O
OH
HO
O
Astaxanthin
Molecular Formula:
Molecular Weight:
CAS No:
C40H52O4
596.82
472-61-7
*G. Britton et al., Carotenoids Handbook, 2004
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NATU R E E V O LV E D PR O TE C TI O N
FRESHLY CUT
ANTIOXIDANT
Any substance that slows down or
inhibits the oxidation of other
substances.
OXIDIZED
OXIDANT
By product of oxygen from energy
production; heat; pollution; stress;
injury; disease and many others.
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N E U T RA L I Z I N G R O S
SOD
３O
2
e－
→
e－
→ H2O2
→
(Hydroxy radicals)
・OH
(Singlet oxygen radical)
O２
↓
LH →
↓
L・
→
↓
Astaxanthin
LH
H2O
↓
LOO・
→
LOOH
↓
L・
QUENCHING
550 x Vitamin E*
40 x -carotene*
(Peroxide
radicals)
Astaxanthin
(Superoxide radical)
↓
１O
2
・O －
2
Ascorbic Acid
Catalase
Vitamin E
(tocopherol)
INHIBITS
PEROXIDATION
1000 x Vitamin E**
*N.Shimizu et al.: Carotenoids as singlet oxygen quenchers in marine organisms, Fisheries Science, 62, 134-137 (1996).
**Miki, W. Pure & Appl. Chem. 63(1);141-46. (ROS: Reactive Oxygen Species)
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ASTAXANTHIN – Neutralizes ROS
SOD
³O₂
O₂˙ˉ
Normal Oxygen
Superoxide radical
Catalase
Vitamin C
H₂O₂
OH˙
Hydrogen Peroxide
Hydroxy radical
L˙
LH
Alkoxy radical
Fatty Acid
Vitamin E
Quenching
550 x Vitamin E
40 x β-carotene
O₂
LOO˙
Peroxyl radical
Inhibits Lipid
peroxidation
1000 x Vitamin E
Radical Chain Reaction
LOOH
Lipid peroxide
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SINGLET OXYGEN QUENCHING
IN VITRO
The second-order rate constants (kq) calculated for the
1O quenching activities of the carotenoids in CDCl (FAT)
2
3
and in a mixture of CDCl3/CD3OD (2:1) (AQ).
Compounds
550
kq×10-9(M-1s-1)
CDCl3
CDCl3/CD3OD (2:1)
Astaxanthin
2.2a
1.8b
Canthaxanthin
－*
1.2
Zeaxanthin
1.9
0.12
Lutein
0.80
－*
Tunaxanthin
0.15
－*
-Carotene
2.2
0.049
-Tocopherol
0.004
－*
40
N.Shimizu et al.: Carotenoids as singlet oxygen quenchers in
marine organisms, Fisheries Science, 62, 134-137 (1996).
* Not examined.
Quenching: Astaxanthin is effective in fat and water based systems.
a550
b40
time more potent than -tocopherol (fat).
times more potent than -carotene (aq).
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SCAVENGING EFFECT OF DIFFERENT
ANTIOXIDANTS IN A LIPOPHILLIC
IN VITRO MODEL
3500
2940
ED 50 value (nM)
3000
2500
2000
1500
960
1000
500
700
400
450
Zeaxanthin
Cantaxanthin
200
0
Astaxanthin
Lutein
ß-Carotene
Vitamin E
Antioxidant
Scavenging: Carotenoids with oxygen groups are most efficient against lipid peroxidation.
Astaxanthin was the most efficient scavenger. Miki, W. Pure & Appl. Chem. 63(1);141-46
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ASTAXANTH I N - I S TH E PU R E ANTI O X I DANT
β-Carotene
Anti- and
Pro-oxidants
Lycopene
Zeaxanthin
Pure
Anti-oxidant
Astaxanthin
0
1
2
3
4
Normalized relative oxidation rates
H.D.Martin, et al. : Chemistry of carotenoid oxidation and free radical reactions, Pure Appl. Chem., 71(12), 2253-2262
(1999).
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THE ASTAXANTHIN SHIELD
Lignell A., & Wood V., Astaxanthin: Radical Cellular Defense. May 2004 Innovations in Food Technology.
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PR O PE RTI E S O F ASTAXANTH I N
Antioxidant Potency
Powerful singlet oxygen quenching
and free radical scavenging activity.
Protects against lipoperoxidation.
Solubility
Spans the membrane bi-lipid layer –
comprehensive protection and
strengthens rigidity
Protection
Forms a protective shield and
defends against continuous free
radical attack at the cellular level.
Safety
Defined as a pure antioxidant unlike
other well known antioxidants that can
become pro-oxidants.
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C O NTAC T
I NTE R NATI O NAL MAR K ETI N G S E CTI O N
4th F L R H A L I F A X O N A R I M O N B L D.
3 – 2 4 –1 0 N I S H I S H I N B A S H I
M I NATO – K U
TO KYO 1 0 5 –0 0 0 3 JAPAN
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Email: [email protected]
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