Transcript 2100 bioanalyzer Series II Kits

Series II 2100 bioanalyzer kits
Superior RNA 6000 assays and reagents
Improvements of the RNA assays
• Improved RNA ladder which is now included in both RNA kits
• Consistently higher sensitivity for the RNA Pico assay
• Increased assay stability for samples with a high salt content for both RNA assays
• New quantitation calibration for both RNA assays
• Larger marker volumes included
• Shelf life initially increased to at least 4 months. Extension is being tested
• Reagents are conveniently packaged according to storage temperature
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New RNA ladder
Series II RNA 6000
Nano assay
Old RNA 6000
Nano assay
RNA ladder now included in the kits
Ladder can also be ordered separately
Provides more intense and stable bands
More reliable integration and quantitation with the 2100 expert software
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Increased sensitivity
50 pg/µL total RNA in water was analyzed using the Series
II RNA 6000 Pico assay
Normal view
Enhanced RNA view with the expert SW
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Salt tolerabilty
Series II assay tolerates high amounts of salt
RNA 6000 Pico - Series II
water
RNA 6000 Pico - old
water
50 mM Tris
10 mM MgCl2
50 mM NaCl
1 ng/µL total RNA was run in different salt-containing media
50 mM Tris
10 mM MgCl2
50 mM NaCl
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Salt tolerabilty
Salt affect overall signal intensity
RNA 6000 Pico - Series II
water
RNA Pico - old
water
50 mM Tris
10 mM MgCl2
50 mM NaCl
- 1 ng total RNA was run in different salt-containing media
50 mM Tris
10 mM MgCl2
50 mM NaCl
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Concentration accuracy
Average Quantification Rat heart Total RNA
700.0
Average Quantification
The quantitation calibration has been
adjusted to better fit UV measures.
Together with the more stable RNA
ladder it provides for a more accurate
and robust quantitation
600.0
500.0
Original
400.0
Series II
300.0
UV Target
200.0
100.0
0.0
400
310
230
170
75
UV Target [ng/µl]
- Rat heart Total RNA (in water) was run in 5 different concentrations (2x each per chip) on 12 replicate chips (6
instruments x 2 different chip lots) – All samples (and Ladder) were pipetted individually as with standard protocol.
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RIN reproducibility
-Burkitt‘s Lymphoma Total RNA was run in 4 different
conc. (1x each per chip) on 18 replicate chips (6
instruments x 3 different chip lots)
Original
Series II
Average RIN
Stv.
% Stdv.
Average RIN.
Stv.
% Stdv.
290 ng/µl
9.9
0.1
0.7
9.8
0.1
0.6
-Mouse Ovary Total RNA (partially degraded) in 5
different conc. (2x each per chip) on 12 replicate chips
(6 instruments x 2 different chip lots)
Original
Series II
Average RIN
Stv.
% Stdv.
Average RIN.
Stv.
% Stdv.
495 ng/µl
4.6
0.3
5.6
4.9
0.4
7.6
RNA Concentration
140 ng/µl 70 ng/µl 35 ng/µl
10.0
10.0
10.0
0.0
0.1
0.1
0.4
0.5
1.1
10.0
9.9
9.9
0.1
0.2
0.1
0.7
1.9
0.9
RNA Concentration
390 ng/µl 290 ng/µl 180 ng/µl 100 ng/µl
5.0
4.7
5.0
5.8
0.4
0.4
0.3
0.4
8.5
6.7
7.3
6.7
4.8
5.0
5.5
5.8
0.3
0.4
0.4
0.4
6.9
8.6
7.1
6.2
RNA Integrity Number (RIN) analysis is unchanged from old to new kits.
The variability is within the expected range across the conc. range.
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RNA 6000 Series II- Specifications
RNA 6000 Nano
RNA 6000 Nano
Series II
Quantitative
range
Total RNA:
25–500 ng/µL
mRNA:
25-250 ng/µL
Total RNA:
25–500 ng/µL
mRNA:
25-250 ng/µL
Qualitative
range
Total RNA:
5–500 ng/µL
mRNA:
25–250 ng/µL
Total RNA:
5–500 ng/µL
mRNA:
25–250 ng/µL
Quantitation
Reproducibility
10% CV
10% CV
20% CV
20% CV*
30% CV*
Quantitation
accuracy
Buffer
compatibility
TE
100 mM Tris
or 125 mM NaCl
or 15 mM MgCl2
RNA 6000 Pico
RNA 6000 Pico Series
II
Total RNA:
200–5000 pg/µL
mRNA:
500-5000 pg/µL
Total RNA:
50–5000 pg/µl (water)
mRNA:
250–5000 pg/µl (water)
TE
50 mM Tris
or 50 mM NaCl
* ladder as sample
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Ordering & pricing
Former Products
Order Number
Series II
Products
Order Number
US Ref.
Price ($)
RNA 6000 Nano
LabChip Kit
5065-4476
RNA 6000 Nano
Kit
5067-1511
544*
RNA 6000 Nano
Cooled Reagents
5065-4475
RNA 6000 Nano
Reagents
5067-1512
296*
RNA 6000 Nano
Ladder
5067-1529
150
RNA 6000 Pico
LabChip Kit
5065-4473
RNA 6000 Pico
Kit
5067-1513
587*
RNA 6000 Pico
Cooled Reagents
5065-4472
RNA 6000 Pico
Reagents
5067-1514
277*
RNA 6000 Pico
Ladder
5067-1535
80
Former kits will be obsolete June 30, 2006
*New price reflects inclusion of the RNA ladder
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Packaging & storage
Storage at 4C
Storage at -20C
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SW update and assay download
Prerequisites to use the Series II RNA kits:
• 2100 expert software B.01.02 or B.02.02 must be installed
• New RNA assays and firmware needs to be installed, use Assay Update
• Assay Update patch can be downloaded free of charge or requested on CD
(fax return form included in every kit)
Three options for software updates available:
• For biosizing users, free of charge update to the intro version of the 2100
bioanalyzer expert SW (Revision B.01.02)
• For B.02.01 users, the upgrade to B.02.02 is FREE of charge
• For all other users (i.e. beta versions), an update to the most current 2100
bioanalyzer expert SW (Revision B.02.02) for a minimal fee is recommended
(order G2946CA)
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Summary
• Improved RNA ladder now included in both RNA kits
• Consistently higher sensitivity for the RNA Pico assay
• Increased assay stability for samples with a high salt content for both RNA assays
• New quantitation calibration for both RNA assays
• Larger marker volumes included
• Shelf life initially increased to at least 4 months
• Reagents conveniently packaged according to storage temperatures
• New Assay Update CD available free of charge
• Only compatible with 2100 expert software B.01.02 or B.02.02
• Offers for SW updates available
More detailed information at http://www.agilent.com/chem/2100-new-assays!
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