Transcript NAFDAC AND AFLATOXIN TESTING

NAFDAC AND AFLATOXIN
TESTING
Oluwabamiwo B. F.
Technical Manager NAFDAC Mycotoxin
Laboratory, Oshodi, Lagos & Denloye Stella
(Director laboratory services NAFDAC)
History of mycotoxin analysis in
NAFDAC
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The IAEA project
EU further training
USAID in-house trainings on QA/QC
UNIDO’s in-house trainings on QA/QC
Preparation for International accreditation
sampling rates for different products
• Sampling: heterogeneous nature of mycotoxin
distribution in food, large samples needed for
analysis, 2- 30kg from which analytical samples
25-100g will be taken
• Sampling must be representative e.g.
• Kernels of a contaminated ear could have
concentrations of aflatoxin ranging from 0 ppb to
• 400,000 ppb, with most kernels having no or very
low amounts of aflatoxin
• if aflatoxin contamination is relatively high, a high
variance will occur among samples
Sampling continued
• Sampling must be conducted throughout the
entire load to be shipped
• The load should possess a unifying lot, contract,
or purchase order number which must be
traceable to each carton, pallet, bin, etc. shipped,
and that is stated explicitly on the certificate.
• Port authorities should be mandated to check
certificates for each batch noting date analysed.
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challenges associated with testing
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Management commitment
Staff commitment
Training & retraining of personnel
Equipment cost (HPLC- about 40,000dolars)
Consumables cost
Carcinogenicity of aflatoxins
Procuring standards (6ml of standard100,000)
Consumable Requirement
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ELISA KITS
Reference standards
Reference materials
Class A glassware
Automatic pipettes
Authenticity of results
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Method verification
Participation in proficiency testing
Use of certified reference materials
In-house qc samples: spiking and recovery
sources, end uses and intended
markets
• Products tested by NAFDAC are sampled at
the port, warehouses of importers and
exporters, (Industries small and large scale)
• Most commodities are packaged and labeled
except export and certain individuals
requesting for testing which is done either
free or at a fee
• The products are meant for the supermarkets,
local markets and exports
time, cost and payment for testing
• Time frame for analysis is one month
• Export 3 weeks
• Payments………(prices)
adequacy of lab services in Nigeria
• Nigeria does not have several laboratories
testing for aflatoxins,
• NAFDAC is striving to be a regional laboratory
for aflatoxin testing
• Accreditation process is on going
role of public versus private labs
• Public laboratories test products before they
are registered by NAFDAC, few laboratories
are analysing using ELISA method e.g. Animal
Care and a few others
• Standard private laboratory that can test for
aflatoxin is still a challenge in Nigeria
changes needed to improve situation
• Cooperation between all the Ministries and
Agencies charged with the responsibility of
monitoring food
• Re defining mandates
• All arms of government from local
government, state and the Federal fulfilling
their roles in food safety
import alerts in major foreign markets
Peanuts
Kulikuli
2007
3
2
2008
2
1
2009
1
2010
1
2011
1
G.nut
oil
Maize
Cereal
product
s
1
2
1
2012
1
8
4
1
Melon
Ogbono
Durum
beans
Spices
17
4
8
1
9
2
7
3
8
3
8
1
1
1
10
2
3
59
Ginger
Ehuru
seeds
Total
35
1
1
17
11
1
1
15
11
13
13
9
2
1
1
102
%
7.8
3.9
1.0
2.0
2.9
57.8
12.7
8.8
2.0
1.0
1.0
What happen to rejects
• Border rejection Destruction – 60 cases
• Information and attention – withdrawal from
market – 9 cases
• Re-dispatch – 17 cases
• Others – exhausted
• In all cases no distribution is allowed within
the EU