Transcript Restriction Map 1

Restriction Map: 1
1 cut
2 cuts
1 cut
PstI
PstI +
CcoIV
CcoIV
7000
3000
7000
PstI
3Kbp
4000
7Kbp
CcoI
4Kbp
Restriction Map: 2
1 cut
EcoRi
2 cuts
EcoRI +
BstEII
1 cut
900
100
BstEII
900
200
100
100
800
200
900
100
700
200
BstEII
EcoRI
800
900
1,000
100
Restriction Map: 3
2 cut
3cut
1 cut
XhoI
XhoI +
FstII
FstII
1,000
1,000
3,000
5,000
2,000
14,000
11,000
3,000
11,000
14,000
XhoI
XhoI
11,000
or
1,000
17,000
3,000
FstII
2,000
3,000
5,000
GO TIME!
Restriction Map 1
Construct a plasmid restriction map of the
following digest. Include cuts and fragment sizes.
EcoRI
EcoRI +
EcoRI = 2 sites
HindIII
HindIII = 1 site
HindIII
7,000
6,000
10,000
3,000
3,000
10k
10k
1,000
10k
1.) Linear or Plasmid ?
2.) What’s the Size? =10K
3.) How many cuts? = 3 sites
Linear: Fragments = cuts + 1
Plasmid : Fragments = cuts
4.) Easy Cuts First! =HindIII
5.) Find relationships!
6.) Rotate
HindIII
1K
7k
6K
EcoRI
10K
3k
10k
EcoRI
Restriction Map 2
Construct a plasmid restriction map of the following
digest. Include cuts and fragment sizes.
HhaII = 1 site
HhaII
HhaII +
XhoI
XhoI
6,000
4,000
2,000
6,000
XhoI = 1 site
HhaII
4K
6K
2K
XhoI
Restriction Map 3
A RIVER runs from Stanton to Fredericksburg. Two
Cabins are located on this stretch of river. The
Teelin’s cabin and the Larson’s cabin are 5 km apart.
The Larson’s cabin is 8 km from Stanton while the
Teelin’s cabin is 11 km from Fredericksburg.
Using the data, make a map of the river and the
cabins located along it. Use a horizontal free line to
represent the river and symbols to represent the
cabins. Label the symbols and intervals between
symbols with the measurements of distance between
the cabins.
– How far is the Teelin’s cabin to Stanton?
– How far is the Larson’s cabin to Fredericksburg?
– Is there an alternate Map?
8 km
11 km
5 km
8 km
Fredericksburg
Teelin’s
Larson’s
Stanton
How far is the Teelin’s cabin to Stanton? 13Km
How far is the Larson’s cabin to Fredericksburg? 16Km
Is there an alternate Map? You Betcha , See Below!
11 km
Now how far is the Teelin’s cabin to Stanton? 3Km
Now how far is the Larson’s cabin to Fredericksburg? 6Km
Fredericksburg
Larson’s
Teelin’s
Stanton
5 km
Restriction Map 4
There are towns along highway 28 running east to
west. Using the data below, make a map of highway
28 and the towns located along it. Use a free
horizontal line to represent highway 28 and symbols
to represent towns. Label the intervals between the
towns with the measurements of distance.
•Freeville : Mt. Crumpit
160 km
•Mt. Crumpit : Whoville
200 km
•Waterville : Freeville
120 km
•Whoville : Waterville
240 km
•Mt. Crumpit : Waterville
40 km
•Freeville hunters get to start each hunting day 8
min. sooner than Waterville hunters.
Whoville
200K
Mt. Crumpit
40K
Waterville
120K
Freeville
West
East
Restriction Map 5
Construct a Linear restriction map of the following
digest. Include cuts and fragment sizes.
SamIII
13,000
9,000
5,000
SamIII +
TrpII
TrpII
10,000
9,000
5,000
17,000
10,000
27K
SamIII = 2 sites
TrpII = 1 site
27K
3,000
13K
27K
SamIII
9K
SamIII
5K
17K
TrpII
3K
10K
10K
Restriction Map 6
Construct a plasmid restriction map of the following
digest. Include cuts and fragment sizes.
CabII = 3 sites
CabII
CabII +
MtGI
MtGI
6,000
3,000
4,500
2,500
8,000
2,000
1,000
1,500
10K
MtGI = 2 site
MtGI
1,500
10K
500
2K
1,000
500
10K
CabII
MtGI
10K
4,500
2,500
8K
CabII
1,000
CabII
Restriction Map 7
3.
There is a trail that runs along a mountain ridge in eastern Vermont
from James Camp to Camp Five. Using a horizontal line to represent
the trail and dots to represent climbing features. Label the intervals
between climbs with the measured distance.
•
•
•
•
•
•
•
•
•
Blood Lake : Washington Peak
Washington Peak : Misery Rock
Hangman's Cliff : One Way Jacks Trail
James Camp : Jackson's Thrill
Hangman’s Cliff : Misery Rock
Washington’s Peak : Jackson’s Thrill
Jackson’s Thrill : Misery Rock
One Way Jacks Trail : Jackson’s Thrill
Blood Lake : Camp Five
•
Jackson’s Thrill should be climbed in the sun before the sun sets
behind Washington’s Peak
3 km
4.5 km
1 km
2 km
9.5 km
4 km
.5 km
10 km
6.5 km
Camp V
One Way Jack’s Trail
3.5K
Hangman’s Cliff
1K
Blood Lake
2K
Washington’s Peak
3K
Jackson’s Thrill
4K
Misery Rock
.5
K
James Camp
1.5K
Cow Person
samurai
Pirate
Superhero
Problem set 1: #1
EcoRI
10,000
EcoRI +
BamHI
8,000
2,000
EcoRI
BamHI
10,000
2K
10K
8K
BamHI
Problem set 1: #2
EcoRI
8,000
4,000
EcoRI +
BamHI
BamHI
6,000
4,000
2,000
EcoRI
BamHI
2K
12,000
12K
4K
6K
EcoRI
Problem set 1: #3
EcoRI
8,000
4,000
EcoRI +
BamHI
6,500
4,000
1,000
500
BamHI
EcoRI
BamHI
1K
6,500
5,500
4K
12K
6.5K
500
EcoRI
BamHI
Problem set 1: #4
14,000
7,000
7,000
4,000
2,000
8000
ClaI
HindIII
ClaI
ClaI
11,000
8,000
2,000
HindIII
HindIII
ClaI
ClaI +
HindIII
8,000
2000
4000
=2 sites
= 1site
7000
#5
Problem
set
1:
HindIII
15000
18,000
BamHI =1 site
13,000
HindIII
3000
7000
HindIII
10,000
6,000
BamHI
BamHI
15,000
+
BamHI
15,000
7,000
6,000
3,000
HindIII
HindIII
= 2 sites
6000
#6
Problem
set
1:
Smal +
Pvul
Pvul
Smal
8,800
8,000
11,800
8,000
5,500
8,700
6,700
4,500
7300
6000
3,800
4200
PvuI
5500
3200
3000
SmaI = 4 sites
= 4 sites
3000
2800
5500 3200
4500
PvuI
SmaI
SmaI
PvuI
PvuI
2800
SmaI
2000
SmaI
PvuI
2200
2000 2200 3000 3800 8000
Problem set 2: #7
EcoRI
6,000
3,000
1,000
EcoRI +
BamHI
4,500
2,500
1,500
500
BamHI
BamHI
8,000
2,000
1,500
EcoRI
BamHI
500
4,500
2,500
EcoRI =3 sites
BamHI = 2site
1,000
EcoRI
EcoRI
Problem set 2: #8
EcoRI
EcoRI +
BamHI
BamHI
BamHI+
HindIII
HindIII
HindIII +
EcoRI
18,000
9,000
11,500
9,000
16,300
14,500
5,000
8,000
9,000
8,700
8,700
5,000
4,000
5,000
8,000
4,500
3,500
3,500
1,500
4,000
3,500
2,200
1,500
2,800
1,800
HindIII
HindIII
3,500
8,000
4,500 9,000 1,000 1,800
2,200
4,000
EcoRI
EcoRI
EcoRI
1,500
BamHI
BamHI
1,000
5,000 1,500
Problem set 2: #9
11,000
7,000
6,000
3,000
1,000
6,000
EcoRI =2 sites
BamHI = 1site
1,000
EcoRI
EcoRI
9,000
6,000
3,000
BamHI
BamHI
EcoRI
EcoRI +
BamHI
8,000
8,000
3,000
Problem set 2: #10
HindIII
HindIII
+
EcoRI
ClaI
+
EcoRI
ClaI
14,000
9,000
17,000
12,000
11,000
14,000
19,000
9,000
8,000
6,000
11,000
9,000
5,000
4,000
3,000
4,000
6,000
HindIII
BamHI
6,000
5,000
3,000
ClaI
BamHI
EcoRI
EcoRI
EcoRI
+
BamHI
5,000
4,000
Quick Draw Δ (linear)
15,000
15,000
BkaI
18,000
13,000
BkaI
16,000
SnaTII+
BkaI
15,000
13,000
3,000
SnaTII
SnaTII
3,000
31,000
13,000
Quick Draw Σ (Plasmid)
TtHII
8,500
TtHII +
ZtoPI
8,000
500
ZtoPI
8,500
TtHII
ZtoPI
500
8,500
8,000
Quick Draw Ω (linear)
HmmI
40
10
HmmI
130
15
10
HmmI
115
SpaZIII
+ HmmI
100
30
15
10
SpaZIII
SpaZIII
100
30
155
15
Quick Draw β (Plasmid)
RamII
8,500
2,500
RamII +
PhaTI
8,500
PhaTI
11,000
8,500
2,000
500
11,000
RamII
500
2,000
PhaTI
RamII