Transcript Zinc Response to Flourescent Indicators

Zinc Response to Flourescent
Indicators
• There is increasing evidence that Zinc
plays a role as a cellular messenger.
• We are able to measure this through the
development of sensitive fluorimetric
probes to measure zinc extracellularly and
intracellularly.
• It is important when studying Zinc to make
sure the probe is specific and selective to
Zn and be able to control other ions such
as Ca and Mg in the solution being tested.
• Measuring Zn in samples with probes
designed for Ca and Mg is complicated
because Zn has to compete with the
binding of Ca and Mg to the probe.
• The fact that many don’t take notice of the
Zn fluorescent probes affinity to Ca and
Mg and this might explain why other
laboratories are having discrepancies
when measuring synaptically released
Zinc.
• Some of the Zn fluorescence indicators to
be tested are: FluoZin-1, Newport Green,
FluoZin-3, zinpyr-2 and a new probe from
Japan Zn AF-2.
• Also selective Ca fluorescence indicators
such as Fura-2, Calcium Green-1 and
Fluo-3 will be tested for both Ca and Zn.