Iodimetric Titration of Vitamin C
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Transcript Iodimetric Titration of Vitamin C
By: Chris Lloyd and Katy Salter
Background Information
Needed in the body to form collagen in
the bones
Absorbed by the body but can not be
stored
Lack of vitamin C results in Scurvydisease that causes brown spots on the
skins and gums will soften to the point of
possible losing your teeth
Introduction
Tested levels of Vitamin C…
Time: Day After Purchase vs. 2 Weeks After
Temp: Room Temperature vs. Fridge
Storage: Open Container vs. Sealed
What We Did…
Made an Iodine solution
Titrated 25mL’s of orange juice sample
until the endpoint
Used 1% starch solution as indicator
What We Used…
Tropicana Orange Juice
No Pulp, Non Concentrate
Vitamin C = 150% Daily Value
60 mg Daily
One Bottle(355mL) = 90 mg
Theoretical grams of vitamin C per 25mL
sample = 0.006338g or 6.338mg
Vitamin C Levels of Orange Juice
1,6
1,4
1,48
1,48
1,51
1,49
1,4
1,28
Amount of Vitamin C (mg)
1,2
1,22
1
0,8
0,6
0,549
0,4
0,2
0
Day 1, RT,
Opened
Day 1, Fridge,
Opened
Day 1, RT,
Sealed
Day 1, Fridge,
Sealed
2 Weeks, RT,
2 Weeks,
2 Weeks, RT,
2 Weeks,
Opened
Fridge, Opened
Sealed
Fridge, Sealed
Vitamin C Levels of Orange Juice with Theoretical
7
6
6,338
Amount of Vitamin C (mg)
5
4
3
2
1,48
1,48
1,51
1,49
1
1,22
1,28
2 Weeks,
Fridge,
Opened
2 Weeks, RT,
Sealed
1,4
0,549
0
Theoretical
Amount
Day 1, RT, Day 1, Fridge, Day 1, RT, Day 1, Fridge, 2 Weeks, RT,
Opened
Opened
Sealed
Sealed
Opened
2 Weeks,
Fridge,
Sealed
Calculations
Actual Amount of Vitamin C per Sample:
Concentration of Standard=Concentration of Sample
(26.7/0.2515)=(6.27/x)
X=0.059g/L
Concentration of Sample x Volume of Sample=Actual grams in Sample
0.059*0.025=0.00148g
Conclusion
Best Way to Store Orange Juice
Sealed in the fridge
Worst Way
Opened at room temperature
Two factors that will preserve Vitamin C
Sealed or in the fridge