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1 Starting a Biotech Program 2 Instructors Stan Hitomi Coordinator – Math & Science Principal – Alamo School San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CA Bio-Rad Curriculum and Training Specialists: Sherri Andrews, Ph.D. [email protected] Leigh Brown, M.A. [email protected] Damon Tighe [email protected] 3 Why Teach Biotechnology? An opportunity to teach science in a way that results in – Science literacy – Technological skills – Career awareness – Critical thinking – Student engagement Students see the direct application of – Biology – Chemistry – Physical science – Math Students learn – Work ethics – Team work, collaboration skills – Communication skills, technical writing – Laboratory techniques 4 Things to consider before starting a Biotechnology Program What type of program are you interested in? 1. Add activities to present curriculum 2. Start a new course in the science department – Semester – Year 3. Academy/Pathway – CTE Program – Project/Problem based – 1 – 4 years 5 Things to consider before starting a Biotechnology Program What kind of course/program are you looking to start? -Technical - Basic science - manufacturing/career based/skills Based (CTE) Who is your target student population? - High School - Tech Prep - ROP/CTE - College/Entry level training Duration - One semester, one year, two year - Pathway, Program, Academy 6 Things to consider before starting a Biotechnology Program 7 What are the goals and objectives? Partnerships …you are not alone Create an advisory board to help develop your program: Who can I ask for advice? Partner with other educators and schools and programs: Who is in my region? - Community College - Universities - Local industry (2+2+2 programs) Partner with local industry and potential internship sites - Pharmaceutical R&D,QC - Manufacturing R&D,QC - Government Funded Agencies - Academic labs/Medical Facilities 8 Developing Curriculum What curriculum will you use? Existing curriculum •DNA Science (ISBN 0-87969-636-2) •Molecular Biology & Biotechnology (978-1-5558-471-7) •Biology and Biotechnology (1-55581-304-6) •Biotechnology (0-7638-2282-5) Modeled after another program •AP Biology (Cindy Gay, CO) •Kirk Brown website (www.jkirkbrown.com) •George Cachianes (http://www.lincolnhigh.net/academy/biotech/overview) Build your own, Combo 9 Why reinvent the wheel?… Some model programs: High School Programs: Kirk Brown, Tracy High School, Tracy, CA Xan Simonson, Mesa HS Biotech Academy, Mesa, AZ George Cachianes, Lincoln High School, San Francisco, CA Holly Welch Choffin Career and Technical Academy Youngstown, PA Eric Dybas Spanish River HS Boca Raton, FL June Carmelego Santa Fe HS Alachua, FL Community Colleges: Trish Phelps, Austin CC Diana Spencer, Tulsa CC Bill Woodruff, Alamance CC Bridgette Kirkpatrick, Colin County CC Jim DeKloe, Solano CC Kate Levine, David Palmer, Contra Costa CC Elaine Johnson, City College of San Francisco, Bio-Link National Center www.bio-link.org University/Research Institution Outreach Programs Kristi DeCourcy, Fralin Biotech Institute Virginia Balke, Delaware Technical and Community College Angela Eeds, Vanderbilt University Nancy Hutchison, Science Education Partnership, Fred Hutch 10 Developing Curriculum Focus of the Course • Textbook based course • Lab based course • Independent projects • Combination Focus within the curriculum • Health/Biomedical • ROP/CTE • Ag Science Possible tools to implement to help your students • Specific skills • Independent research • Internships (summer or school-year) • Job shadowing opportunities 11 Developing Curriculum What knowledge and skills do I want my students to have? Examples • Learn to use equipment safely and appropriately • Learn sterile technique • Know how to make solutions • Keep a lab notebook • Know structure and function of DNA • Know how to manipulate DNA • Understand enzyme structure and function What training or type of degree or certification is needed for local and state requirements? •Professional Development •Certification programs 12 Lab Activities for existing curriculum 13 14 Sample Syllabi Kirk Brown, Tracy High School http://www.tracy.k12.ca.us/thsadvbio/ 15 Funding Collaborations with University and Outreach programs - NSF and HHMI grants to Universities, Community Colleges, Research Institutes have an outreach component (Partnering institutions) Other Grants or Funding Sources: - Toshiba Tapestry Grants - State Grants/CTE - Local companies - National companies Target, Starbucks, McDonalds Fundraising Activities: - Family Science Night - Genes in a Bottle™ explorer.bio-rad.com 16 Teaching Resources - Grant Writing Information - Grantors and Directories Finding Funds for Biotechnology Studies Grant Writing Workshop 17 Cloning and Sequencing Explorer Series 18 Advanced Comparative Proteomics Kit I: Protein Profiler Module Comparative Proteomics Kit II: Western Blot Module PV92 PCR Informatics Kit GMO Investigator™ Kit* Real-Time PCR Applications Intermediate Biofuel Enzyme Kit - NEW ELISA Immuno Explorer™ Kit Forensic DNA Fingerprinting Kit Analysis of Precut Lambda DNA Kit Restriction Digestion & Analysis of Lambda DNA Kit pGLO Kit SDS-PAGE Extension Crime Scene Investigator PCR Basics™ Kit* Introductory Genes in a Bottle™ Kit pGLO™ Bacterial Transformation Kit Green Fluorescent Protein Chromatography Kit Secrets of the Rainforest™ Kit Size Exclusion Chromatography Kit Got Protein?™ Kit Microbes and Health Kit Building Step by Step Equipment • What equipment do I have? • What equipment do I need? Equipment wish list: • What is my budget? Year to Year Growth Plan: What equipment will allow you to grow your program with time? (Think long-term) Year 1, 2, 3 … 19 Complete Biotechnology Teaching Resources Standards Aligned Kit Based Lab Activities •Materials for 8 complete student workstations Detailed Curriculum Manuals and Teaching Guides •Teacher guide/Student guide •Step-by-step preparation and set-up •Inquiry-based adaptable lessons to match grade and skill level and maximize student involvement •Extension activities Teaching Resources •PowerPoint Presentations •Animations •Resource Links •CTS •explorer.bio-rad.com Professional Development/Training •CTS Research Grade Equipment 20 Technical Support The Pipeline PRIMARY/SECONDARY EDUCATION HIGHER EDUCATION STIMULATE & DEVELOP INTEREST IN SCIENCE SKILLS, APPLICATIONS, CAREER OPTIONS Elementary/ Middle School High School Community/ vocational college University RESEARCH INSTITUTIONS BASIC & APPLIED RESEARCH Academic Industry LIFE SCIENCE CAREER PATH Kits & equipment Lab extensions Kits & equipment Lab extensions Student research Real-Time PCR Proteomics: 2D Gel Electrophoresis Chromatographic separations Need Help? Your CTS can help you! Curriculum Training Specialists [email protected] (858) 201-9301 [email protected] (510)314-3748 [email protected] http://explorer.bio-rad.com 22 Genes in a Bottle Students extract and preserve their own DNA LIFE SCIENCE STANDARDS & SKILLS: Cellular structures – membrane, organelles DNA – location, solubility – genetic information – extraction from tissue Enzyme properties & function – use of protease to aid in extraction 23 pGLO™ Bacterial Transformation Students transfer a jellyfish gene to bacteria Can regulate the expression of GFP 24 LIFE SCIENCE STANDARDS & SKILLS: Central Dogma of Molecular Biology – DNA>RNA>Protein>Trait – Genetic engineering Prokaryotic cells – Cell biology, genetic transmission – Antibiotic resistance Microbiology techniques – Sterile technique – Growth media Chromatography Kits: GFP Chromatography purify the bioluminescent protein (extension to pGLO) Secrets of the Rainforest™ study the ethical issues of biotechnology Size Exclusion Chromatography Separate vitamin B12 and hemoglobin using chromatography 25 LIFE SCIENCE STANDARDS & SKILLS: Chromatography – Protein properties – Tools for protein purification Creating a commercial product – Scientific, ecological, ethical and legal issues of biotechnology Biomolecules – Physiological function – Naturally pigmented molecules – Maintenance of deleterious alleles (e.g. sickle cell anemia) Microbes and Health – “What Causes Yogurtness?” Isolate the microorganisms from yogurt and inoculate milk, linking the “sick” milk, to the causative microbe 26 LIFE SCIENCE STANDARDS & SKILLS: Isolate yogurt-causing bacteria – Follow Koch’s postulates – Microbiology and human disease – Biotechnology and food production Learn laboratory microbiology skills – Study food microbiology and bacterial metabolism Inquiry Based – Do different brands of yogurt contain different microbes? – Directly linked to students daily lives Forensic DNA Fingerprinting Compare Crime Scene DNA with 5 Suspects to determine “Who Done it?” Restriction Analysis of Lambda DNA Use restriction enzymes and gel electrophoresis to analyze DNA LIFE SCIENCE STANDARDS & SKILLS: Allelic differences in populations – DNA as a unique identifier – Heredity Restriction enzymes – Function and properties – Origin in bacteria and use in genetic engineering – Plasmid mapping Agarose Gel Electrophoresis – Separation of molecules using electrical current – Size based on distance traveled, creating buffers Micropipetting techniques 27 Crime Scene Investigator PCR Basics™ Students learn to use the polymerase chain reaction (PCR) and DNA electrophoresis to determine the genotypes of 5 DNA samples Simulate real-world crime lab techniques using one of the Short Tandem Repeat (STR) loci commonly used in forensic typing. 28 LIFE SCIENCE STANDARDS & SKILLS: Allelic differences in populations – DNA as a unique identifier – Use of PCR in DNA profiling – Polymorphic loci and multiple alleles – Non-coding DNA sequences Agarose Gel Electrophoresis – Separation of molecules using electrical current – Size based on distance traveled, creating buffers Micropipetting techniques PV92 PCR Informatics Students isolate and examine the DNA fingerprint of their own DNA LIFE SCIENCE STANDARDS & SKILLS: Population Genetics – Hardy Weinberg equilibrium – Evolution, migration, speciation – Bioinformatics using Cold Spring Harbor allele server Polymerase Chain Reaction – Uses in forensics, archeology, research, disease diagnostics Agarose Gel Electrophoresis – Separation of molecules using electrical current – Size based on distance traveled, creating buffers 29 GMO Investigator™ Students extract and amplify DNA from grocery store foods LIFE SCIENCE STANDARDS & SKILLS: Agriculture and environment – Pesticides and herbicides – Population growth and environmental challenges – Plant biodiversity and ecosystems Molecular Biology – DNA replication and PCR – Genetic transformation to create GMOs – Control of gene expression (in foreign hosts) 30 Got Protein?™ Kit Determine the protein concentration in common beverages such as milk, sports drinks and more Engages students – they bring in their samples of choice LIFE SCIENCE STANDARDS & SKILLS: Proteins – Essential molecules in all living cells Bradford Assay – Coomassie Blue dye binds to protein and allows for quantitation and detection – correlation between the amount of blue color and the amount of protein Spectrometry – Use of spectrophotometer – Apply Beer’s Law Inquiry based - which foods/drinks provide more proteins? – Directly links to students daily lives 31 ELISA Immuno Explorer™ Students simulate spreading an infectious disease and detect origin Types of ELISA Tests included: • Tracking the spread of a disease (epidemiology) • Detecting specific antigens in a sample • Diagnosing past exposure to a disease 32 LIFE SCIENCE STANDARDS & SKILLS: Immunology – antigen-antibody interaction – virology – infectious diseases Enzyme Properties – enzyme-substrate interaction – colorimetric detection Real world applications – Pregnancy, drug, HIV testing – GMO and environmental testing – bioterrorism Comparative Proteomics Kits: Protein Profiler – Module Evolution Wet Lab – create protein profiles for different fish species 33 LIFE SCIENCE STANDARDS & SKILLS: Molecular evolution – Natural selection and genetic diversity – classification Polyacrylamide Gel Electrophoresis – protein separation techniques Module 1 Comparative Proteomics Kits: Western Blot – Module Takes Protein Profiler to the Next Level – identify the specific protein using antibodies LIFE SCIENCE STANDARDS & SKILLS: Molecular evolution – Natural selection and genetic diversity – classification Western blotting – Explore immunodetection – Protein separation techniques – Antibodies as tools Module 2 34 Biofuel Enzyme Kit Guides instruction on enzyme kinetics and biofuel energy sources LIFE SCIENCE STANDARDS & SKILLS: • Enables both qualitative and quantitative measurements of reactions - Determine the rate of reaction in the presence or absence of an enzyme - Determine the effect of temperature, pH, enzyme concentration, substrate concentration on the rate of reaction - Test the ability of mushroom extracts to increase the rate of reaction • Aligns with AP Biology Lab 2 35 Cloning and Sequencing Series Genomic DNA Extraction DNA Precipitation DNA Quantitation Bioinformatics Sequence Data Editing Contig Assembly Intron-Exon Prediction GAPDH PCR Nested PCR Degenerate primers Exonuclease Sequencing Automated sequencing Gel Electrophoresis DNA Gel Interpretation Band Identification Standard Curve Use Plasmid Miniprep Restriction Enzyme Digestion Gel Electrophoresis PCR Purification Size Exclusion Chromatography Microbial Culturing Antibiotic Selection Sterile Technique 36 Cloning Direct PCR cloning Transformation Ligation Webinars • Enzyme Kinetics — A Biofuels Case Study • Real-Time PCR — What You Need To Know and Why You Should Teach It! • Proteins — Where DNA Takes on Form and Function • From plants to sequence: a six week college biology lab course • From singleplex to multiplex: making the most out of your realtime experiments explorer.bio-rad.comSupportWebinars 37