Transcript Title

The ePlantLIBRA Database:
Plant Food Supplements -Bioactives
Composition, Benefit and Adverse Effects
Jenny Plumb,
Paul Finglas, Mairead Kiely, Jackie Lyons, Simone Bell,
Erik Nørby, Karin Nørby, Miles Thomas, Roland Poms
& Patrizia Restani
10th IFDC, Granada, Spain, 14 September 2013
Outline
PlantLIBRA project
History of the bioactives
databases
ePlantLIBRA structure and
content
Users and Uses
10th IFDC Granada
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Project: Plant Food Supplements –
intake, benefit and risk assessment
Seventh Framework Programme
Theme 2: Food, Agriculture and Fisheries, and Biotechnology
Large-scale integrating project for specific cooperation actions dedicated to
international cooperation partner countries (SICA)
The PlantLIBRA project
(www.plantlibra.eu) aims to foster
the safe use of food supplements
containing plants or botanical
preparations, by increasing
science-based decision-making by
regulators and food chain
operators. To make informed
decisions quality-assured and
accessible information and better
tools are required e.g. an up-todate and reliable database.
21.07.2015
http://eplantlibra.eurofir.eu
EU Definition of a food supplement:
“‘food supplements’ means foodstuffs the purpose of which is to supplement
the normal diet and which are concentrated sources of nutrients or other
substances with a nutritional or physiological effect, alone or in
combination, marketed in dose form, namely forms such as capsules,
pastilles, tablets, pills and other similar forms, sachets of powder,
ampoules of liquids, drop dispensing bottles, and other similar forms of
liquids and powders designed to be taken in measured small unit
quantities”
Plant Food
Supplements
Homoeopathic
Medicines
MEDICINAL
LAW
FOOD LAW
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Traditional
Herbal
Medicines
otc
Foods with
plants added
Food Supplement
Plant based
foods
Fortified Food
Food
Food Supplement Directive 2002/46
http://eplantlibra.eurofir.eu
OTC & Rx
Medicines
PlantLIBRA Work Packages
Intake estimation
of PFS
Using eplantlibra
to calculate intake
of bioactive
compounds
Produce new data:
Analysis of bioactive
composition in
botanical ingredients
and PFS
Research into
methods for plant
identity
Methodology of
benefit assessment
for PFS,
application and
validation
Investigation on
adverse effects to
botanicals and PFS
acute and long-term:
Integrate the
information on risks
and benefits, in order
to support sciencebased decisions.
Dissemination
Consumer behaviour
and beliefs
10th IFDC Granada
http://eplantlibra.eurofir.eu
Definition - Bioactive Compounds
“Inherent non-nutrient constituents in plant foods with
putative health-promoting and/or toxic effects”
 Beneficial: May help to promote optimal
health and to reduce the risk of chronic
disease
 Plant-based bioactive compound classes
including:




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Anthroquinones (e.g Aloe)
Ginkgolides (e.g Ginkgo)
Flavonoids (e.g. grapes, berries, onions, tea)
Glucosinolates (e.g. Brussels sprouts,
cabbage, broccoli)
http://eplantlibra.eurofir.eu
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eBASIS/ePlantLIBRA History:
Bioactive Substances Information System
NOTIS (naturally occurring
toxicants information system)
IFR (CD Rom)
EU-AIR Nettox
1995-1997
1990
TOXIP (naturally occurring
toxicants in food plants) DTU
1990s
1995
EU BASIS 1999-2001
Fully functional database CD-Rom
300 food plants (12 European
languages), 5000
compositional records, 75
toxicological studies
2000
Split and continued as
EuroFIR BASIS on-line
database(EU FP6 2005-2010)
Nortox BASIS (Nordic Council of
Ministers)
2005
eBASIS: EuroFIR NEXUS 2010
2010
To further additions to eBASIS via
BACCHUS 2012-2016 FP7
Cardiovascular benefits from food
bioactives
2015
Databases combined eBASIS:
EFSA Bioactive Constituents of
Food plants 2009
ePlantLIBRA 2010-2014 EU FP7
bioactives in plant food
supplements
Aim: To develop, test and apply a sustainable
integrated meta-database of biologically active
compounds, residues and contaminants
ePlantLIBRA: A sustainable, reliable, flexible and fit-for-purpose internetdeployed database, providing a unique comprehensive resource on PFS for
researchers, health professionals, health educators, the food industry and policy
makers
Beneficial Bioeffects
Compositional data
Adverse Effects
Contaminants/residues
Bibliographic information
Plant information
http://eplantlibra.eurofir.eu/
PFS information
Additional information
ePlantLIBRA Structure
ePlantLIBRA functions
Data inputting:
Data reporting:
Via 5 online systems
• Composition data
• Beneficial data
• Adverse effects
• PFS information
• Plant information:
user led data retrieval
software system,
searchable by:
compound, food,
biological effect:
•Composition
•Bio-effects
•PFS info
•Plant details
•Contaminants
Compilation process
Selection of
1
• Evaluators
• Compound classes
• Food plants/PFS 3
2
Storage of quality evaluated data5
Document
Reference selection 1
Identification of data sources,
relevant plants/PFS
relevant compound classes
Useful data in
reference?
YES
Continue
evaluation
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YES
SOP
Correct?
NO
Check on evaluators
4
data entry
No - STOP
evaluation
Submission to
’holding database’
Data entry by evaluators2 4
Attribution of quality score to
3
all original data
http://eplantlibra.eurofir.eu
DBM
requests revision
Reporting: Composition data
VIEW REPORT
Reporting: Composition data
Download
as Excel file
Link to
input form
Link to plant
details
Download Refs
to EndNote
Link to reference details
and original publication
Beneficial Biological effects
VIEW REPORT
Link to
input form
Link reference details
and original publication
Adverse effects reporting
Contaminant information
Contaminant/
residue
Level
Unit
Analysis/
Regulation
10th IFDC Granada
385 plants
within the
database have
been
categorised and
encoded against
commodities in
the MoniQA
database
Linked to
Plant
Plant Part
eplantlibra contents
Composition eplantlibra data: 2781 inputs, 148 papers assessed, 234
compounds, 70 PFS plants/products + 22 000 entries from eBASIS (500
references
Beneficial bioeffects eplantlibra : 100 papers , 78 entries + 771
information sets/300 biomarkers from eBASIS (450 references)
Adverse bioeffects: 200 records covering 32 plants/PFS
Contaminants 385 plants categorised, encoded against commodities in
MoniQA
396
plants
10th IFDC Granada
500
compounds
44 PFS
http://eplantlibra.eurofir.eu
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Users and Uses of eplantlibra
Food
Authorities
Policy
makers
Food
Industry
10th
• Regulatory issues,
• Science based
decision making,
• Preparation of
health claim
dossiers
• Benefit/risk
assessments
IFDC Granada
Health
professionals
Poisons
centres
Regulators
Hospitals
• Estimating
exposure levels,
• Epidemiological
studies,
• Supporting
submissions to
research
http://eplantlibra.eurofir.eu
Researchers
Pharmacists
• New product
development
• User friendly info
on botanicals
• Easily accessible
info on adverse
effects
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Examples of use:
Benefit/Risk
assessment
Integration of databases:
OPASNET: an internet based
interface where users can access,
combine, by plant and
compound, and discuss
information for risk benefit
assessment.
http://en.opasnet.org
European
Poisons Centres
by
STIC
Establish a Network
with Poison Centers
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Example of use:
Consumption Survey, Intake calculation
200
150
100
50
0
10th IFDC Granada
http://eplantlibra.eurofir.eu
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Conclusion
The ePlantLIBRA database represents:
o
State of the art for food composition and biological effects of
plant and PFS based bioactives data
o
A unique comprehensive resource for scientists, policy
makers. the regulatory sector, epidemiologists, health professionals,
health educators, and the food industry.
o
A valuable database for the investigation of Plant Food
Supplements and botanicals and their health relationships
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http://eplantlibra.eurofir.eu
21.07.2015
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Acknowledgements
PlantLIBRA WP 6 members and 3rd Parties
The research leading to these results has received funding from the
European Community's Seventh Framework Programme (FP7/2007-2013)
under grant agreement n° 245199. It has been carried out within the
PlantLIBRA project (website: www.plantlibra.eu). This report does not
necessarily reflect the Commission views or its future policy on this areas
[email protected]
www.ifr.ac.uk/fooddatabanks
Prioritised Plant species
Aloe ferox
Borago officinalis
Boswellia serrata
Camellia sinensis
Cassia angustifolia
Cassia obtusifolia
Cassia senna
Cassia tora
Cinnamomum verum
Cinnamomun zeylanicum
Citrus aurantium
Citrus limon
Citrus sinensis
Cynara scolymus
Echinacea pallida
Echinacea purpurea
Epimedium brevicornum/sagittatum
Foeniculum vulgare
Ginkgo biloba
Glycine max
Harpagophytum procumbens
Hypericum perforatum
Lycium barbarum
Matricaria recutita
Melissa officinalis
Passiflora incarnata
Peumus boldus
Plantago ovata
Rhamnus purshiana
Salvia hispanica / Salvia columariae
Serenoa repens
Silybum marianum
Valeriana officinalis
Vitex agnus-castus
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http://eplantlibra.eurofir.eu
Examples of PFS in database
Artichoke based supplement
Bilberry product
Black cohosh product
Boswellia product
Cacao product
Calendula product
Cinnamon product
Dandelion product
Devil's-claw supplement
Echinacea products
Flaxseed/linseed product
Ginkgo product
Ginseng product
Green tea dietary supplement
Guarana product
Liquorice product
Medicinal fennel supplement
Milk-thistle product
Passionflower product
Pomegranate supplement
Red clover supplement
Rhodiola Rosea (Golden Root) Product
Rhubarb supplement
Saw Palmetto product
Senna supplement
Soy supplement
St. Johns wort products
Turmeric product
Valerian product
Yerba mate product
Categories of biomarkers covered to date
Examples of categories of biomarkers covered
Haemostatic function (e.g. platelet aggregation, P-selectin expression)
Endothelial function (e.g. increased release of prostaglandins, NOx)
Homocysteine metabolism (e.g. reduced levels of plasma folate)
Oxidative damage (e.g. measuring TBARS, superoxide dismutase)
Inflammation (e.g. C-reactive protein, interleukins 1-13)
Lipid profiles (e.g. triglycerides, HDL-cholesterol)
Glucose metabolism (e.g. plasma glucose, insulin, proinsulin)
Antioxidant status (e.g. ascorbic acid, beta-carotene, lycopene)
Liver function tests (e.g. bile salts, total bilirubin, AST, ALT)
NORWICH PROJECT MEETING – MAY 2013
ePlantLIBRA cf EFSA Compendium
ePlantLIBRA
EFSA Compendium
Aim
Database on PFS and plant
bioactive compounds composition
data, health effects , toxic effects,
contaminants, residues. Quality
evaluated
Compendium of botanicals that
have been reported to contain
toxic, addictive, psychotropic or
other substances of concern.
Design
Online searchable database
Table and guidance document
Botanical Info
Common and scientific names,
pictures,
Scientific names
Products
Yes
No
Composition
Data
Yes, detailed, quality evaluated
Some, brief
Beneficial Data
Yes, biomarkers, effective levels
No
Tox Data
Yes, detailed, quality evaluated
Yes, brief
Search Dates
Will be up to date
To March 2009
Compounds
Yes, searchable by compound
Not searchable
References
Yes, linked, downloadable to
endnote
Yes
Quality
Documented
Not documented
Adverse effects input forms
From Brasov May 2011 to Rome September 2012
EuroFIR = adverse effects studied in
Human, Animals, in vivo and in vitro
ePlantLIBRA = adverse effects reported in
Human Case reports or Human trial studies
NORWICH PROJECT MEETINGS – MAY 2013
Selection of Plants for data input - 2
Papers describing adverse effects due to the
ingestion of the PFS/Plants have been
collected and rated by WP4
Only papers for those of the plants where
the adverse effects have been classified with
the causality: certain, probable or possible
have been retrieved and uploaded in
ePlantLIBRA for data input
NORWICH PROJECT MEETING – MAY 2013
New features under
development – an example
Plant
Latin name
Exporter (source)
Camomile
Chamaemelum nobile
(L.) All.
Germany
Camomile
Chamaemelum nobile
(L.) All.
The Former Yugoslav
Republic Of Macedonia
Onion
Cantharellus cibarius Fr.
France
var. cibarius
Allium cepa L.
Morocco
Onion
Allium cepa L.
Ukraine
Onion
Allium cepa L.
Ukraine
Chanterelle
NORWICH PROJECT MEETINGS - MAY 2013
Reported
commodity
Issue (residue or contaminant)
Acetamiprid, Carbofuran, Chlormequat,
Chamomile
Dimethoate, Fonofos, Glyphosate, Heptachlor,
flowers
Methamidophos, Methiocarb, Oxydemetonmethyl, Triazophos
Acetamiprid, Chlorpyrifos, Dimethoate,
Chamomile
Methomyl, Monocrotophos, Omethoate,
flowers
Procymidone
High radioactive concentration (220 Bq/kg) in
Wild mushrooms
Chanterelles
from Onions
cadmium in fresh wild onions
lead (0.15 mg/kg - ppm) in fresh onions from
from Onions
Ukraine
lead (0.33 mg/kg - ppm) in fresh onions from
from Onions
Ukraine