Transcript Slide 1

Kinesin-5 nano-motors in cell division
Leah Gheber
Departments of Clinical Biochemistry and Chemistry
Ilse Katz Institute for Nanoscale Science and Technology
Ben-Gurion University of the Negev, Beersheba, Israel
Mitotic spindle
human cells – specific kinesin-5 inhibitor monastrol
5 m
control
Leizerman et al., CMLS 2005 (Gheber lab)
monastrol
Kinesin-5 motor proteins
Kinesin-5
+
+
Spindle assembly
Anaphase spindle
elongation
-
Maintenance of bipolar
spindle structure
Our Goal
To understand the mechanisms and regulation
of the mitotic kinesin-5 nano motors
Our Strategy
To correlate between in vivo functions
and in vitro motor activity of specific
kinesin-5 mutants and variants
In vivo assays – protein expression and
quantitative live imaging
Cin8
cin8-5A
Cin8-3GFP
Cin8
26
37
26
37
Cin8-5A-3GFP
Cin8-3A
Microtubule plus-end tracking
Tubulin
Kinesin-5
In vitro - Single molecule fluorescence
motility assay - Kinesin-5-3GFP
People who do the work
Vladimir Fridman
Adina Gerson-Gurwitz
Natalia Movshovich
Collaborators
Maria Podolskaya
Christoph Schmidt – GAUG
Stefan Lakamper – GAUG
Mart Loog – Tartu, Estonia
Liam Holt – UC Berkeley, US
Andy Hoyt – JHU, US
Dina Raveh – BGU, Israel
Rachel Abunie-Masala
Ofer Shapira
Adina
Vladimir
Nurit Schkolnik
Alina Goldstein
Rachel
Nurit
Natalia
Alina
Ofer