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20/07/2015 OCR Gateway Additional Science Biology Unit 4 – It’s a Green World W Richards The Weald School 20/07/2015 B4a: Ecology in the local Environment Taking samples of an ecosystem 20/07/2015 Using different “sampling techniques” we can measure changes in an ecosystem. The two main measurements are: 1) The physical conditions of a habitat (temperature etc) 2) The populations of different species in that habitat Some common ways of measuring… Help! Measuring temp, pH etc Taking animal samples Taking samples using quadrats Population Size 20/07/2015 There are two ways we can easily use to estimate population size: Method 1 – Scaling up If a 1m2 sample contains 10 woodlice how many woodlice are in a field measuring 200m by 300m? 200m x 300m x 10 woodlice per square metre = 600,000 woodlice Method 2 – Capture/recapture A sample contains 10 stoats and a week later a second sample contains 15 stoats of which 2 were in the previous sample. How many stoats are in the population? Population Estimate = No. in 1st sample x no. in 2nd sample Number in 2nd sample previously marked No. of stoats = (10 x 15) / 2 = 75 stoats What assumptions are we making with this method? Ecosystems 20/07/2015 “Ecosystem” is a term used to describe all of the organisms living together as a community in a particular habitat and can often be self-supporting other than the need for an energy source. Ecosystems are constantly changing: Changes in population due to predation, disease etc Physical changes (e.g. temperature change) Changes in ecosystems Pollution Changes in soil conditions Human interference These changes can lead to “zonation” – a gradual change in species distribution over the habitat. Transect lines and zonation 20/07/2015 Here are some population samples taken along a transect line along a shoreline: Ecosystems 20/07/2015 An “Ecosystem” is a physical environment with a particular set of organisms living in it. They can be natural, e.g. a woodland, or artificial, e.g. a greenhouse. A natural ecosystem with high “biodiversity” An artifical ecosystem – biodiversity will be controlled and low B4b: Photosynthesis 20/07/2015 Photosynthesis 20/07/2015 Basically, photosynthesis is the process through which a plant makes its own food using carbon dioxide and water: That’s a nice plant. I’m going to put it in the sun and give it lots of water and air… CO2 H2O 20/07/2015 Photosynthesis – the 4 things you need SUNLIGHT Gives the plant energy CHLOROPHYLL WATER Travels up from the roots CARBON DIOXIDE Enters the leaf through small holes on the underneath The green stuff where the chemical reactions happen Photosynthesis equations Carbon dioxide + _____ Sunlight Chlorophyll 20/07/2015 glucose + _____ Sunlight 6CO2 + 6H20 Chlorophyll C6H12O6 + 6O2 The GLUCOSE produced by photosynthesis is used by the plant for _______ (through ____________). It is stored in the plant as ___________. Words – respiration, starch, water, oxygen, energy Photosynthesis over the ages 20/07/2015 Our understanding of how plants grow has changed over the ages: I’m a Greek scientist and we believed that plants gained mass through taking things in from the soil. I’m Jean Baptista Van Helmont and I showed that plant growth wasn’t possible through nutrient uptake alone. I’m Joseph Priestley and I proved that oxygen is produced by plants. How Photosynthesis works 20/07/2015 Basically, we now understand that the oxygen produced by photosynthesis comes from water: 1) Light is used to split water, releasing oxygen gas and hydrogen ions: HO H22+O C6H12O6 2) Carbon dioxide combines with the hydrogen ions to make glucose: CO2 What is the glucose used for? 20/07/2015 1) Glucose (sugar) can be used to make long chains of insoluble starch… Glucose molecules Starch molecule 2) Glucose can be used to make cellulose for cell walls… Glucose molecules Cellulose 3) Glucose can be combined with nitrates to make proteins (for growth)… Glucose molecules Proteins 4) Glucose can be converted into lipids (fats or oils) to store in seeds… Glucose molecules Lipid structure Limiting Photosynthesis What factors could limit the rate of photosynthesis? 1. Temperature – the best temperature is about 300C – anything above 400C will slow photosynthesis right down 2. CO2 – if there is more carbon dioxide photosynthesis will happen quicker 3. Light – if there is more light photosynthesis happens faster 20/07/2015 20/07/2015 Drawing graphs of these factors 1. Temperature Photosynthesis is controlled by enzymes – these are destroyed at temperatures above 400C 2. Carbon dioxide Photosynthesis increases at first but is then limited by a lack of increase in temp or light 3. Light Photosynthesis increases at first but is then limited by a lack of increase in temp or CO2 Encouraging Photosynthesis 20/07/2015 Using knowledge of limiting factors, explain how plant growth is encouraged in a greenhouse: Photosynthesis and Respiration 20/07/2015 In earlier work we looked at respiration: Glucose + oxygen water + carbon dioxide Photosynthesis is the reverse of respiration: Water + carbon dioxide glucose + oxygen 20/07/2015 Concentration of gas around plant Gas concentrations over 24 hours Oxygen Carbon dioxide Midnight Midday Midnight B4c: Leaves and Photosynthesis 20/07/2015 Structure of the Leaf 20/07/2015 How do each of the following features help photosynthesis? Lots of chlorophyll Large surface area Transparent Thin structure Packed with chloroplasts Network of veins Lots of air spaces Holes B4d: Diffusion and Osmosis 20/07/2015 Diffusion 20/07/2015 Diffusion is when something travels from an area of high concentration to an area of low concentration. For example, consider the scent from a hamburger… The “scent particles” from this hamburger are in high concentration here: Eventually they will “diffuse” out into this area of low concentration: Oxygen passes into cells by diffusion Diffusion Summary 20/07/2015 Diffusion is when particles spread from an area of high concentration to an area of ___ concentration. The particles move along a “concentration _____” and this process takes no _____. Diffusion can be accelerated by increasing the _______ of the particles, which makes them move _______, or by increasing the surface area of the membrane. Words – faster, low, gradient, temperature, energy Osmosis 20/07/2015 Osmosis is a “special kind of ___________”. It’s when water diffuses from a __________ area to a less concentrated area through a partially permeable _________ (i.e. one that allows water to move through but not anything else): Water Sugar solution In this example the water molecules will move from left to right (along the concentration ______) and gradually _____ the sugar solution. Words – membrane, concentrated, dilute, diffusion, gradient Potato cells 20/07/2015 Strong sugar solution Medium sugar solution Weak sugar solution Osmosis of water from leaves 20/07/2015 Low conc. of water Water leaves the plant by diffusing through osmosis into the air spaces and then evaporating. Low conc. of water High conc. of water Turgor pressure 20/07/2015 Consider a plant that’s been well watered: Because there is a _______ concentration of water in the soil than in the ____, water will enter the plant cells through _______ and the cells will become TURGID (“_________”): In this case the stomata will ______ to allow carbon dioxide in and water ___ and photosynthesis will carry on. The cell wall prevents the cell from bursting. Words: out, swollen, higher, soil, osmosis, open Turgor pressure 20/07/2015 Consider a plant that’s been badly watered: Because there is a lower concentration of water in the soil than in the plant water will leave the plant cells through osmosis and the cells will become FLACCID (“soft”): In this case the stomata will close to stop water from escaping through transpiration. Because they are closed carbon dioxide cannot get in and so photosynthesis stops. The cell looks like a deflated balloon – this is called “plasmolysis”. Turgid = “tense” Flaccid = “floppy” Osmosis in Animal Cells Animal cells don’t have a cell wall so if red blood cells are placed in a weaker solution they swell up, whereas if they are put in a more concentrated solution they will shrivel. If they absorb enough water they will eventually burst (“lysis”), whereas if they lose enough water they shrivel up and become “crenated”. These differences to plant cells are caused by the lack of cell wall. 20/07/2015 B4e: Transport in Plants 20/07/2015 Using water Plants need water to do the following: Enable transport of minerals Produce glucose Form fruits Keep the leaves cool Enable growth 20/07/2015 20/07/2015 Close up on the stem - Xylem and Phloem Xylem vessels are made from dead plant cells and are used by the plant to transport water and soluble mineral salts from the roots to the stem and the leaves. Phloem are tubes made from long columns of living cells and are used by the plant to transport dissolved food to the whole plant for respiration and storage. Transpiration 20/07/2015 1) Water evaporates through the stomata 2) Water passes back into the leaf through the XYLEM vessels by osmosis 3) Water is then pulled upwards through the xylem tissue 4) This is replaced by water entering from the root tissue 5) Water enters root hair cells by osmosis to eventually replace the water lost through respiration Factors affecting transpiration 20/07/2015 1) High light intensity This causes the stomata to open thereby increasing the rate of water loss. 2) High temperatures This increases the speed of molecule movement and so speeds up transpiration. 3) Increased wind This will blow the molecules away and so speeds up transpiration. 4) High humidity This decreases the concentration gradient and so slows transpiration down. Measuring Transpiration 20/07/2015 The rate of transpiration can be measured using a “potometer”. Bung to prevent evaporation As the plant takes up water the rate can be measured by determining the speed of the bubble. Water loss 20/07/2015 Water loss through the stomata is biggest on a hot, dry, windy day. Plants that live in these conditions often have a thicker waxy layer. Water loss through leaves 20/07/2015 Carbon dioxide enters a leaf through the ________. These cells are also responsible for controlling the ______ content of the ____… The ______ cells control how wide the stomata opens (if at all). If too much water is being lost through the stomata then the guard cells will _____ to prevent further loss. Water and carbon dioxide enter here No more water and carbon dioxide allowed in Words – water, close, stomata, guard, leaf B4f: Plants need Minerals 20/07/2015 Essential Minerals Nitrates: Used to make amino acids to form proteins Lack of it leads to stunted growth Magnesium: Used to make chlorophyll for photosynthesis Lack of it leads to yellow leaves Phosphates: Used to make DNA and cell membranes Lack of it leads to underdeveloped roots and discoloured leaves Potassium: Used to make enzymes for respiration and photosynthesis Lack of it leads to underdeveloped fruit and flowers 20/07/2015 Active Transport 20/07/2015 Here is a root hair cell: Now consider the concentration of minerals around the cell: Mineral More concentrated Less concentrated Plants need minerals and ____ and these are “taken up” by the root hair cell. The _________ of these minerals inside the cell is ______ than outside so _____ is needed for the process. This process is called _______ transport. Words – active, concentration, ions, higher, energy B4g: Decay 20/07/2015 Recycling ourselves 20/07/2015 Microbes are the key to this – they break down waste and dead bodies so that the products can be used by plants for growth. Microbes work best in warm, moist conditions where there is plenty of oxygen. More about Decay 20/07/2015 Decay is the process of breaking down complex structures into ______ ones. This is done be micro organisms. The rate of decay is affected by the _______ of these microorganisms: 1) Temperature – the micro organisms’ rates of growth and respiration are quickest at around 40OC. They become “_________” at temperatures higher than this. 2) Oxygen – more oxygen means _____ micro organisms. 3) Water – micro organisms grow quickest in ______ conditions. Words – more, moist, simpler, growth, denatured Decomposers 20/07/2015 Microorganisms are called “decomposers”. They are used by humans to help the decay process in compost heaps and sewage treatment works. They are helped by earthworms, woodlice and maggots. These are called “detritivores” – this means they feed on dead organisms and waste and break it down (i.e. increase the surface area) to speed up the decay process. Saprophytes A “saprophyte” is an organism like the shiitake mushroom that digests dead material like dead wood: This mushroom digests dead wood using “extra-cellular digestion”, i.e. the food is digested outside of the cell membrane, often by having enzymes excreted onto it. 20/07/2015 Preventing Food Decay Food can be preserved by limiting the growth of micro organisms. Some examples: 1) Using sterile tins/bottle/cans 2) Using a fridge or freezer 3) Pickling the food 4) Adding sugar or salt 5) Dehydrating the food 20/07/2015 B4h: Farming 20/07/2015 Pests 20/07/2015 Crops can be damaged by pests such as herbivorous insects, weeds and fungal infections. How can the problem be reduced? 1) Using ________ – these will kill _____ but they can build up in plants causing the death of other _______. 2) Introducing natural predators, such as ______ – not as easy as using pesticides and would take _______. Words – ladybirds, animals, insects, longer, pesticides Biological pest control 20/07/2015 Sometimes a food web can be controlled by the introduction of a pest, e.g. Ladybirds feed on aphids A wasp looking for caterpillar larvae What are the advantages and disadvantages of using pests? Accumulation of pesticides 20/07/2015 Insecticides can wash into a stream or lake where they are taken up by microscopic water plants. Consider the food chain: Although the level of insecticides in the plants is small, it will build up through the food chain due to the number of organisms in each stage. In this example, if each plant had “one bit” of pesticide, the bird will have eaten 9 bits. Intensive Farming 20/07/2015 Some examples of intensive farming: Battery farms Fish farms Hydroponics Glass houses Farming Methods 20/07/2015 There are basically two types of farming method; “intensive” and “organic”: Intensive Cost Produce Fertilisers Space Labour done by... Organic Intensive Farming 20/07/2015 Intensive farming can be used to improve the efficiency of energy transfer in a food chain by two ways: 1) Get rid of energy transfer to pests or competing weeds 2) Limit an animal’s movement or keep it warm: Hydroponics 20/07/2015 Hydroponics basically means “growing plants without soil”. Advantages Disadvantages Mineral levels can be controlled Plants have to be supported Reduced risk of disease Expensive fertilisers