Transcript BacAbs kick-off meeting

ASLA Biotech Ltd.
SME partner for EU
www.asla-biotech.com
Established in 1999
8 permanent positions
10 collaborating research fellows
Anatolijs Sharipo, CEO
FP7 meeting 13 SEP 2007
ASLA Biotech Ltd.
currently participates in
two EU FP6 projects
as a partner:
1. COMPUVAC: Rational design and standartized
evaluation of novel genetiv vaccines Integrated
Project
2. BacAbs: Assesment of structural requirements in
complement-mediated bactericidal events
FP7 meeting 13 SEP 2007
our experience: STAGES OF SUCCESS
To find partner
To participate in
proposal
To get finansed
To do the job
FP7 meeting 13 SEP 2007
To find partner
How to find a partner:
1. Personal links: experience in international
research
2. CORDIS databases
- registration and waiting for searchers
- active search
3. National Point for FP grants
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
How to convice coordinator and
other partners in your participation
in the consortium?:
• Demonstrate expertize (you have to have it first)
• Find a nishe (there has to be one in the project)
• Show advantages by:
• your quality
• your budget
• Take initiative if necessary
• Do not take initiative if not necessary
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
Quality as a Partner
• Publications with our participation as authors
• References to our products and services
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Liepinsh E, Barbals R, Dahl E, Sharipo A, Staub E, Otting G. The death-domain fold of the ASC PYRIN domain,
presenting a basis for PYRIN/PYRIN recognition. J Mol Biol. 2003 Oct 3;332(5):1155-63. PMID: 14499617 [PubMed indexed for MEDLINE]
Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G. Solution structure of the R3H domain from human Smubp2. J Mol Biol. 2003 Feb 7;326(1):217-23. PMID: 12547203 [PubMed - indexed for MEDLINE]
Williams NK, Prosselkov P, Liepinsh E, Line I, Sharipo A, Littler DR, Curmi PM, Otting G, Dixon NE. In vivo protein
cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem. 2002 Mar
8;277(10):7790-8. Epub 2001 Dec 12. PMID: 11742000 [PubMed - indexed for MEDLINE]
Liepinsh E, Baryshev M, Sharipo A, Ingelman-Sundberg M, Otting G, Mkrtchian S. Thioredoxin fold as
homodimerization module in the putative chaperone ERp29: NMR structures of the domains and experimental model
of the 51 kDa dimer. Structure (Camb). 2001 Jun;9(6):457-71. PMID: 11435111 [PubMed - indexed for MEDLINE]
Papers referring ASLA Biotech as a labeled protein/aligning reagents
provider: 45 since 2001
Journals: Molecular Cell, Nat Struct Mol Biol., Structure, J Biomol NMR, Protein Sci.,
J. Biol. Chem, J. Mol. Biol., Nucleic Acids Research, Biopolymers, RNA, J. Am. Chem. Soc.,
BMC Cell Biology,
FP7 meeting 13 SEP 2007
www.asla-biotech.com/asla-ref.htm
To find partner
To participate in
proposal
Facilities & Equipment
• Our own facilities
• Access to complicated
equipment
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
Using big academical Institutions
ASLA
BMC
Facilities/Infrasructure
OSI
Scientific environment
Research collaboraton
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
How to win?:
• choose right consortium/coordinator
• trust them
• do the best for the consortium during proposal
• do not put all eggs in the same basket
If lost:
• do not complain
• analyse your negative experience
• start from the step 1
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
“To swim, you have to swim” Mao Dze Dung
“Impossible is nothing” Adidas
“Just do it” Nike
Our recovery of financed grants
versus submitted applications:
• 1 grant of 3 in 2005
• 2 grants of 13 in 2006
• ? grants of 8 in 2007
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
To do the job
EXPERTIZE we bring to EU consortia
Products
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NMR aligning reagents
NMR protein standards ready to use, sealed in NMR tubes
Antibodies
Custom Services
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labeling proteins by 13C, 15N and 2H
artificial gene synthesis
molecular biology services
custom immune reagents
Complex customer oriented studies
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artificial gene synthesis -> specific reagent production
subcellular localization for the molecule of interest
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
To do the job
Protein Expression and Purfication
• proteins from native sources
• bacterially expressed proteins:
•fusion proteins: GFP, GST, RSA, His-tag,
MBP, STREP
•non-tagged proteins
• proteins expressed in insect/mammalian cell
cultures
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
Protein Expression and Purfication
Optimization of expression:
temperature, medium, E.coli strain etc.
E.Coli growth scales:
Flask expression
4L 4ML fermentor
20L fermentor from subcontractors
Labeling: 15N, 13C, 2H in all combinations,
selective labeling available
Purification:
•FPLC/HPLC: ion exchange, size exclusion, reverse phase
•Affine chromatography
Analysis:
•PAAGE – coomassie/silver stain
•Mass spectrometry
•WB/IP/ICC validation
To do the job
To find partner
To participate in
proposal
To get finansed
To do the job
Protein Expression and Purfication: labeled ubiquitin NMR standards
Intens. [a.u.]
15N-1H HSQS 15N-labeled ubiquitin
8874.149
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To find partner
To participate in
proposal
To get finansed
To do the job
Custom Immune Reagents
Polyclonal Sera & mAbs
Antigens:
• proteins from native sources
• bacterially expressed proteins
• proteins expressed in mammalian cell cultures
• peptides coupled to different carriers
fusion proteins: GFP, GST, RSA, His-tag, MBP,
STREP
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
To do the job
NMR studies
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Concept: We provide contract studies
on protein structure determinations
and other experiments related to the
protein structure and interactions. We
express, label by stable heavy
isotopes and purify the protein of your
interest and finally, we are able to
determine its structure by NMR
spectroscopy methods. The service is
provided on commercial basis.
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Equipment: a permanent access to 4
chanel ( 1H, 13C, 15N, 2D) NMR
spectrometer Varian INOVA 600 MHz
equipped with cryoprobe located at the
Latvian Institute of Organic Synthesis
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NMR expertize: Dr Edvards Liepinsh,
OSI, NMR of proteins and nucleic
acids
FP7 meeting 13 SEP 2007
To find partner
To participate in
proposal
To get finansed
To do the job
BE FLEXIBLE
• VERY OFTEN THE TASK MIGHT BE
CHANGED DURING THE PROJECT
• ONE NEEDS TO BE READY FOR NEW
TASKS IN FRAMES OF THE
EXPERTIZE
• TO BE READY TO GET NEW
EXPERTIZE
FP7 meeting 13 SEP 2007
Marina Koulikovska, PhD
Anatoly Sharipo, PhD
Ainars Leonchiks, PhD
Gunda Grinberga
Marija Teveleva
Sabine Skipare
Zhanna Rudevica
Gunda Grinberga
Victoria Logina
Dima Zhulenkovs
Andris Zeltinsh, PhD
Latvian Biomedicine Research and Study Centre, Ratsupites 1 LV-1067, Riga, LATVIA
Thank you
FP7 meeting 13 SEP 2007