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Internal assessment "The gift of fantasy has meant more to me than my talent for absorbing positive knowledge." "The secret to creativity is knowing how to hide your sources." 1 IA in Biology has 3 (5) components 1. 2. 3. 4. 5. Design (D) – marked twice Data Collection and processing (DCP) -marked twice Conclusion and Evaluation (CE) - marked twice (Manipulative Skills – assessed over the course of the year) Personal Skills – assessed in the Group 4 project) 2 Design has 3 ‘Aspects’ Aspect 1: Defining the problem and selecting variables Aspect 2: Controlling the Variables Aspect 3: Developing a method for collection of data 3 Look at Page 23 of your IB Introduction 4 Aspect 1: Defining the problem (research question) and selecting variables 5 Design involves 1. 2. 3. 4. 5. 6. A Research Question (RQ): focussed, with observations and limits Variables: relevant independent and dependent Recognition of uncertainties and sources of error Effective methods to control variables Effective methods to measure variables Sufficient measurements/ data for analysis 6 Important ‘Regulations’ imposed by IB The teacher is only allowed to provide an ‘openended problem’ to investigate The teacher is only allowed to suggest a ‘general’ research question’ 7 Students can NOT be Given a focused research question Told the likely outcome of the experiment Told which independent variable to select Told which variables to hold constant 8 We will work over the next 2 weeks on the following ‘General’ research questions: 1.How do different concentrations of a specific solution affect osmosis in plant or vegetable tissue 1.How do different concentrations of a specific solution diffuse across a semi-permeable membrane? 9 Simple Design ‘tips’ It is easier to adapt a ‘known and used method’, than to invent a whole new method! It is NOT appropriate to derive a known or existing law or constant Be original, but try to keep your method simple! 10 Your Design is YOUR design If a school sends in two identical design IA’s then the whole class will be marked down for IB grading All design projects MUST BE YOUR OWN WORK You must think of your own research question Your description of variables must be comprehensive 11 There are 6 sections to your design IA 1. 2. 3. 4. 5. 6. 7. Research question Background information Accurately defined variables (independent, dependent, controlled) Apparatus Risk assessment Assessment of potential errors and uncertainties Materials and Method used 12 …so how do you come up with an ‘original’ Research Question? 13 Remember your Scientific Method, and essential components of experimental Design Observation (Inference) Research Question/ Hypothesis/Null Hypothesis Data – quantitative and qualitative Variables Controlled experiment Control group Research Question Your research question must be PRECISE and it must be MEASURABLE The simplest methods: What is the effect of x (independent variable) on x (dependent variable)? Is x correlated with y? We must have a principle (hypothesis)/ question that we can objectively test using quantitative methods 16 Referenced background Information is essential The RQ must be based on background knowledge and observation The RQ can be presented as a hypothesis, based on biological reasoning A brief referenced introduction will provide background information and justification for your Research Question An example! You have been asked to design an experiment investigating factors that affect biological enzyme activity Some preliminary research (Google) has explained that lead may impede plant growth through acting on catalase: http://oas.uco.edu/03/paper/fenska.htm You decide to investigate this… What would an appropriate Research Question be? 19 Research Question Does lead nitrate inhibit the activity of catalase? Is this sufficiently precise? No! we don’t know how much lead nitrate is being used, nor how we will measure catalase activity… 20 Precise RQ: Do concentrations ranging from 0.01 M – 0.25 M lead nitrate in the reaction mixture inhibit the rate of production of oxygen from hydrogen peroxide by potato catalase at room temperature? Better, but we still don’t know how rate of oxygen production is being measured… 21 Precise RQ: Is the production of a head of bubbles of oxygen from hydrogen peroxide inhibited by concentrations of 0.01 M – 0.25 M lead nitrate in the reaction mixture? 22 ASPECT 2 OF DESIGN: CONTROLLING THE VARIABLES 23 Dependent Variable What you will measure Must be specified clearly Include units Will form the Y-axis of your graph Do NOT use ‘rate’ unless you have actually measured rate in xxs-1 Do not use ‘amount’ – use ‘volume’ or ‘mass’… 24 Independent Variable What you will manipulate Forms the X-axis of your graph The range must be chosen carefully..aim for several integers You may wish to include ‘negative controls’ 25 Controlled variables Variables which could potentially affect the dependent variable and thus affect the data You must detail these and keep as many as possible constant Examples include: temperature, total volume of reaction mixture, pH, concentration of solutions, surface area, time, light intensity, source of biological material used 26 Uncontrolled variables? Ideally, you should have none! If you cannot eliminate certain environmental conditions, you must monitor and record them 27 Identify the experimental VARIABLES Dependent – what you measure (Y-Axis) Independent – what you change (X- axis) Controlled – what remains constant (Uncontrolled – variables which may be hard to control) Ideally you should have all variables controlled, excepting one dependent and one independent variable Example: Effect of Lead Nitrate on catalase activity Independent variable: presence or absence of lead nitrate in the reaction mixture Dependent variable: rate of production of oxygen Does this delineate the variables adequately? Is ‘rate of oxygen production’ truly the dependent variable? 29 Example: Effect of Lead Nitrate on catalase activity Independent variable: presence of lead nitrate at concentrations of 0.01 M – 0.25 M in the reaction mixture Dependent variable: change in % oxygen in closed chamber, measured using Vernier® probe 30 ASPECT 3: DEVELOPING A METHOD FOR COLLECTION OF DATA 31 How much data is enough? How much data is enough? That all depends on the experiment! The IB love 5 X 5 experiments! The teacher cannot tell you: • HOW to collect the data • How much data to collect… 33 The classical five by five model 5 different test groups Repeat the procedure for each group 5 times Not all experimental procedures need a 5 X 5 model! Apparatus and materials The appropriate apparatus must be selected and stated Quantity (SI units, volume/mass), size of measurement equipment must be stated You must use the most precise means of measuring your specific materials Uncertainty of apparatus and materials must be discussed A picture or photo of your apparatus is always GREAT! 35 Risk Assessment! Scientific experiments shouldn’t be dangerous! You must point out potential dangers – sharp scalpels, Bunsen burners, boiling water, potentially caustic chemicals You must detail how you will avoid potential dangers 36 Materials and Methods 1. 2. Write in the passive voice, in the future tense Use paragraphs or bullet points 37 What are Standard International (SI) ? Measurement SI Units/accepted non-SI units Distance m, cm, mm Mass Kg, g, mg Volume dm3, cm3 Concentration Moles, millimoles Time Seconds, minutes 38 Design exemplar: Investigation into the effects of milk on growth rate of young rats A classic experiment on young laboratory rats, carried out 100 years ago Group A were fed a baked dry diet only Group B were fed with the same diet plus 5cm3 milk each day 39 Research Question: How will milk affect the rats’ growth? Milk No milk 40 Independent and Dependent variable Independent – the presence of milk in the diet Dependent – the growth rate Was this measured? Accurate? 41 Independent and Dependent variable I - The presence or absence of 5 cm3 of milk as a daily supplement to a dry diet D – the mass of each rat measured every 5 days 42 Controlled variables 1. 2. Gender Age Precise? Shows control? 43 Method to control variables 1. 2. The rats will be fed daily The cages will not be moved Controlled? Repeatable? Make a list of other controlled variables 44 Method to control variables 1. 2. Every day, each rat will be given 20g of dried food, the food remaining from the previous day will be removed and weighed to record the amount of food consumed each day The room will be air-conditioned at a steady temperature of 20 oC ± 1oC 45 Controlled variables 1. 2. 3. 30 male rats will be tested in each group The testing will begin when the rats are 20 days old, after weaning has finished The groups will be kept on the diet for 20 days and then the diets will be switched for a further 20 days (crossover study) Sufficient results 46