Transcript Slide 1

Development of BioProducts for
Animal and Human Health
J. J. Lin, Ph.D.
CEO/Chief Scientist
Imagilin Technology, LLC
Imagilin Technology, LLC
Who Are We:
• Biotechnology company, Frederick,
Maryland
• Technology platforms of probiotics
as biomedicines
• Innovative probiotic products
Emerging of Antibiotics Resistance Bacteria-Superbugs
January 16, 2006,
The Washington Post
Definition of Probiotics
The Food and Agricultural Organization of the United
Nations (FAO):
"live microorganisms administered in
adequate amounts which confer a beneficial
health effect on the host.“
Market Size of Animals
• Farm Animals
- USA, US $79.9 Billions Meat
production
• Pet Foods
-USA, 15.7 Billions
• Pet Nutrition Supplements
-US $1.00 billion, 17-22 % growth rate
Innovative Technology
• Novel strains selection systems:
-In vitro and in vivo selection of strains
with enhanced immune responses.
• Microencapsulation of probiotics:
- preservation of probiotics under
harsh environment
• Probiotics as biomedicine:
-against infectious diseases related to
digestive disorders
Effects of different pH on growth of P. acidilactici
and L. acidophilus in vitro
Pediococcus
acididlactici
pH 6.2
pH 2.0
pH 1.5
Lactobacillus
acidophilus
Poultry Coccidiosis
E. tenella
E. acervulina
E. maxima
E. tenella
No toxic effects of P. acidilactici on
broilers infected by Eimeria acervulii
Weight Gain(g) / 1-9 dpi
Group: EA-5,000
450
400
350
300
250
200
REG
0.01% P.a..
0.05% P.a..
0.1% P.a..
% P. acidilactici
0.2% P.a.
0.4% P.a..
Effects of P. acidilactici on broilers
infected by Eimeria acervulii
Effects of Pediococcus acidilactici on broiler chickens* infected with Eimeria acervulii.
USDA, ARS, Beltsville, MD
(X 108
Oocysts)
(gm)
Oocyst
Shedding
Wt. Gain 3
400
390
2.5
380
2
370
360
1.5
350
1
340
330
0.5
320
310
Control
No P. a.
0.1% P. a.
0
Control
No P. a.
0.1% P. a.
*: 20 chickens per group; chickens were orally infected with 5,000 sporulated oocytes and fed
with or without P. acidilactici (P.a.).
Effects of serological responses of P. acidilactici
on broilers inefected by Eimeria Spp.
Serum ELISA for α-Mic2pQE9 (1:16)
Absorbance (450nm)
St
an
da
r
P. d D
a iet
P. 0.1
a %
0.
2%
ET
ET St a
- n
ET P.a dar
-P .-0 d
.a .1%
.-0
.2
EA
%
EA St
-P and
EA .a a
-P .-0. rd
.a 1%
.-0
.2
%
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Treat
Product Lines
• Nutraceutical
Products:
- MITOMAXR : Premium Probiotics for Dogs,
Cats, Small animals
- MITOBIRDTM : Premium Probiotics for Birds
- MITOFISHTM: Premium Probiotics for Fish
- MITOBOOSTTM : Natural Antioxidant and
Immune Enhancers for Pets
• Farm
Animal Feeds Additives:
-MITOGROWTM: Premium probiotics for Poultry
-MITOSWINETM: Premium probiotics for Pigs
Summary
•Two scientific publications in peer review journals
•Lead to develop the probiotic product lines to
pet and farm animal business
•Develop international business partnerships in
Japan, China, Hong Kong, Taiwan, and Brazil
Effects of different temperature on growth of P.
acidilactici and E. faecium in vitro
P. acidilactici
E. faecium
37C
65C*
* Treated bacteria at 65C for 3 hrs, plated onto MRS plate ,and incubated at 37C overnight
Characteristics of P. acidilactici
• Natural existence in animals and humans
• Generally Recognized As Safe (GRAS) by FDA
• Enhanced immune responses
• Less sensitive to exposure of oxygen than
Lactobacillus and Bifidobacterium.
• More resistant to heat than
Lactobacillus and Bifidobacterium.
• More resistant to acidic environment
Trends
• Emerging Antibiotics resistant SuperBugs
-Abandon Antibiotics
-Alternative Medicines
• Demanding Nutracetutical products
-Healthy food
-Nutrition supplement
-Therapeutical effects
• Requiring Green (organic) foods
-Antibiotics free
-Growth hormone free
-Pesticide free
Performance of chicks(Cobb) fed or not with probiotic or
growth promoter during all the breeding period (1 to 42 days of age)
Brazil, Sao Paulo
Animal feed
Live weight (g)
Feed
DWG*
Feed
conversion
(g)
consumption
(g/g)
PEI
Mortality*
(%)
(g)
Probiotic full
2.781
1,595
66,22
4.329
385a
7,14a
No additive
2.752
1,614
65,52
4.267
352b
13,21b
With promoter
2.723
1,621
64,84
4.315
368b
7,85a
With enzyme
2.737
1,641
62,8
4.305
362b
10,35ab
Means followed by different letters in the column are different among themselves by the Tukey test (p<0,05), 40
birds per treatment
PEI: Viability X Weight gain/ convertion rate X 10.
Effect of Levofloxacin the growth of Pediococcus acidilactici
Control
50 ug/ml
100 ug/ml
200 ug/ml
4 hours
Mixed 300 mg P. acidilactici with 3 ml of MRS medium, added 50 ug/ml, 100 ug/ml,
200 ug/ml of levofloxacin, followed by incubating at 37 C for two nights, and plated
100 ul at 10,000 dilution onto MRS plate. The colony formation was obtained after
incubated overnight at 37C
Poultry Coccidiosis