Transcript STM Applications in Biomedicine

In The Name of Allah
STM Applications in Biomedicine
Po o r i a G i l l
PhD Of Nanobiotechnology
p o o r i a g i l l @ y a h o o. c o m
Nanoscopy of Nanostructured Biomolecules
 Structural Analyses
 Interactiomics
 Partial Sequencing
 Immobilization Characterization
 Peptide Characteristics
 Microbial Characteristics
 Viral Characteristics
…
Single strand of calf thymus DNA deposited along a surface step of HOPG.
(50 x 50 nm, constant current mode, current 0.1 nA, bias voltage 500
mV.)
Methods In Molecular Biology, Vol 22. Microscopy, Opt/cat Spectroscopy, and Macmscop/c Technrqoes Edlted
by: C Jones, I3 Mulloy, and A H. Thomas Copynght 01994 Humana Press Inc., Totowa, NJ.
STM of λ-DNA (GeneRuler DNA) on HOPG
P. Gill, B. Ranjbar, R. Saber. IET Nanobiotechnol., 2011,Vol. 5, Iss. 1, pp. 8–13.
3D image of a single antibody (IgG) molecule after the filtering and coloring process,
which shows orientation of this molecule after physical adsorption on the rigid
surface from the hinge region imaged by NAMA-STM
R. Saber, S. Sarkar, P. Gill, B. Nazari, F. Faridani. Scientia Iranica F (2011) 18 (6), 1643–1646.
3D image of a single antibody (IgM) molecule, imaged by NAMA-STM. (b) Standard
configuration of human immunoglobulin M with pentameric domains
R. Saber, S. Sarkar, P. Gill, B. Nazari, F. Faridani. Scientia Iranica F (2011) 18 (6), 1643–1646.
STM comparison of passive antibody adsorption and biotinylated
antibody linkage to streptavidin on microtiter wells
STM images of antiferritin antibodies
passively adsorbed to a microwell surface
STM images of biotinylated antiferritin
antibodies immobilized to a streptavidin
coated microwell surface
Davies et al., Journal of Immunological Methods, 167 (1994) 263-269.
Individual Peptide Structures Visible by STM
Reconstructed surface topography of coated T4 polybead capsomeres; (a) the TEM and (b) STM
representations. The slight variation in the representation may be due to the overlying carbon film,
which is observed by STM but not by TEM. Height range is 2.3 nm. (Reprinted with permission from
Stemmer et al., 1989.)
M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron,Vol. 26, No. 4, pp. 347-362, 1995.
Reconstructed surface topography of coated T4 polybead capsomeres; (a) the TEM and (b) STM
representations. The slight variation in the representation may be due to the overlying carbon film,
which is observed by STM but not by TEM. Height range is 2.3 nm. (Reprinted with permission from
Stemmer et al., 1989.)
M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron,Vol. 26, No. 4, pp. 347-362, 1995.
STM image of coated (a) sheath and (b) hoops from M. hungatei. Bars: x, y =
100 nm; z = 8 nm.
M. FIRTEL and T. J. BEVERIDGE. Scanning Probe Microscopy in Microbiology. Micron,Vol. 26, No. 4, pp. 347-362, 1995.
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